Synchronized parasites at a parasitaemia level of 2–3% were collected and lysed with 0.15% saponin. After washing twice with 1% PBS, the cells were resuspended in 1× SDS loading buffer (Bio-Rad, Hercules, CA, USA). Since the molecular weight of PfArp4 and PfArp6 is approximately 62 kDa and 121 kDa, respectively, an 8% SDS-PAGE gel was used to isolate the proteins. The antibodies used in the experiment were rabbit anti-HA (1:2000; Abcam, Cambridge, UK), mouse anti-Ty1 (1:1000; Sigma-Aldrich), and rabbit anti-PfAldolase (1:1000; Abcam, Cambridge, UK). An ECL western blot kit (GE Healthcare, Atlanta, GA, USA) was used to develop the blots.
Rabbit anti pfaldolase
Manufactured by Abcam
Sourced in United Kingdom
Rabbit anti-PfAldolase is a primary antibody used for the detection of PfAldolase, an enzyme involved in the glycolytic pathway of the malaria parasite Plasmodium falciparum. This antibody can be used in various immunoassays to identify and quantify the presence of PfAldolase.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti ty1, by Merck Group (2 mentions)
Sds loading buffer, by Bio-Rad (2 mentions)
Rabbit anti ha, by Abcam (1 mentions)
Ecl western blot kit, by GE Healthcare (1 mentions)
Ecl western blotting kit, by GE Healthcare (1 mentions)
Rabbit anti histone3, by Abcam (1 mentions)
Mouse anti ha, by Roche (1 mentions)
2 protocols using rabbit anti pfaldolase
1
Isolating PfArp4 and PfArp6 proteins
2
Western Blot Analysis of Parasite Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total parasite extracts were prepared by 0.15% saponin treatment and resuspended in 1x SDS-loading buffer (Bio-Rad), then separated on 8 ~ 12% SDS-PAGE gel according to the molecular weight of targeted proteins, and subjected to Western blot analysis. The commercial antibodies used in this study were mouse anti-Ty1 (1:500, Sigma), mouse anti-HA (1:2000, Roche), rabbit anti-PfAldolase (1:1000, Abcam), and rabbit anti-Histone 3 (1:1000, Abcam). ECL western blotting kit (GE healthcare) was used to develop blots.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!