Vevo 2100 system

Manufactured by Fujifilm

Sourced in Canada, United States

The Vevo 2100 system is a high-frequency, high-resolution micro-ultrasound imaging platform designed for preclinical research applications. The system utilizes advanced transducer technology to capture real-time, high-quality images and data from small animal models.

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Lab products found in correlation

30 mhz transducer, by Fujifilm (8 mentions) Ms400 linear array transducer, by Fujifilm (7 mentions) Ms 400 transducer, by Fujifilm (6 mentions) Vevo 2100, by Fujifilm (6 mentions) Ms550d transducer, by Fujifilm (5 mentions) Chloral hydrate, by Thermo Fisher Scientific (4 mentions) 550 s probe, by Fujifilm (4 mentions) Ms250 13 to 24 mhz scanhead, by Fujifilm (4 mentions) Vevolab software, by Fujifilm (4 mentions) Isoflurane, by Baxter (4 mentions) Ms 400, by Fujifilm (4 mentions) Matlab, by MathWorks (4 mentions) 40 mhz linear probe, by Fujifilm (3 mentions) Ms 250 transducer, by Fujifilm (3 mentions) Ms250, by Fujifilm (3 mentions) 30 mhz probe, by Fujifilm (3 mentions) Isoflurane, by Abbott (2 mentions) Ms400 18 38 mhz transducer, by Fujifilm (2 mentions) Aerrane, by Baxter (2 mentions) Mk2206, by Merck Group (2 mentions)

398 protocols using vevo 2100 system

Echocardiographic Assessment of Cardiac Function in Mice

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Short axis M-mode and long-axis B-mode echocardiography was performed in
anesthetized mice using the Vevo 2100 system (VisualSonics, Toronto ON). The
following parameters were measured as indicators of function and ventricular
remodeling: left ventricular end-diastolic volume (LVEDV), ejection fraction,
end-diastolic left ventricular anterior wall thickness (LVAWTd), end-diastolic
left ventricular posterior wall thickness (LVPWTd), and left ventricular mass
(LV mass). Lean and diabetic mice underwent Doppler echocardiography and tissue
Doppler imaging at 6 months of age to assess diastolic function using the Vevo
2100 system (VisualSonics). Transmitral LV inflow velocities were measured from
apical 4-chamber view by pulsed-wave Doppler. Peak early E (E-wave) and late A
(A-wave) filling velocities and E/A ratio were measured according to the
guidelines of the American Society of Echocardiography (29 (link)). Tissue Doppler Imaging of the mitral annulus was
obtained from the apical 4-chamber view. A 1.0-mm sample volume was placed
sequentially at the medial mitral annulus. Analysis was performed for assessment
of early (E′) diastolic velocity, and the E/E′ ratio was
calculated. Views and data were exported for offline calculation using Vevo 2100
quantification software (Vevo 2100 v.1.6.0). The offline analysis was performed
by a sonographer blinded to study groups.

Hanif W., Alex L., Su Y., Shinde A.V., Russo I., Li N, & Frangogiannis N.G. (2017). Left atrial remodeling, hypertrophy and fibrosis in mouse models of heart failure. Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology, 30, 27-37.

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Echocardiographic Assessment of Cardiac Function in Mice

Cited 4 times

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Echocardiography was conducted in anaesthetized (2% isoflurane) mice using a Vevo 2100 system (VisualSonics, North America). The long and short axes were acquired in B-mode, and measurements were performed in M-mode.
STE analyses of the parasternal long-axis B-mode loops were performed using a VisualSonics Vevo 2100 system as previously described [[24] (link), [25] (link), [26] (link), [27] (link)]. Parasternal long-axis B-mode videos were recorded, and three-dimensional regional endocardial radial strain images were obtained over four consecutive cardiac cycles. Global left ventricular (LV) endocardial longitudinal and radial strain in the long axis and circumferential and radial strain in the short axis (peak strain %) with the maximum opposing wall delay were evaluated (calculated by Vevo Strain software).

An D., Zeng Q., Zhang P., Ma Z., Zhang H., Liu Z., Li J., Ren H, & Xu D. (2021). Alpha-ketoglutarate ameliorates pressure overload-induced chronic cardiac dysfunction in mice. Redox Biology, 46, 102088.

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Magnetic Targeting of Stem Cells

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All animal studies were performed in accordance with the Institutional Animal Care and Use Committee (IACUC) at the University of California, San Diego (UCSD). Mice were anesthetized with 1–2% isoflurane. SIO in 50% Matrigel (40 μL of 10 mg mL⁻¹) were injected into the left ventricle myocardium close to the apex with a 31 gauge BD insulin syringe. The injection was guided by ultrasound imaging with a 40-MHz-centered linear transducer (MS550) on a VEVO 2100 system (VisualSonics, Fujifilm) in real time and assisted by an injection arm that came with the VEVO 2100 system. Immediately after the injection, the mouse was cleaned and a magnetic harness was put onto the mouse with the magnet close to the heart apex. The harness was adjusted not to hinder normal movement of the animal, and the mouse was monitored twice daily. For the SIO control group, no magnetic harness was used on the animals. For the magnet control group, animals were put on magnetic harness but with no injections. After 7 days, the iron content in the hearts was determined with inductively coupled plasma mass spectrometry (iCAP RQ-ICP-MS, Thermo-Fisher). ICP-MS was used here because the concentration of the elements could be low and ICP-MS is more sensitive than ICP-OES.

Chen F., Zhao E.R., Hableel G., Hu T., Kim T., Li J., Gonzalez-Pech N.I., Cheng D.J., Lemaster J.E., Xie Y., Grassian V.H., Sen G.L, & Jokerst J.V. (2019). Increasing the Efficacy of Stem Cell Therapy via Triple-Function Inorganic Nanoparticles. ACS nano, 13(6), 6605-6617.

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Cardiac Function Evaluation Post-MI and Infusion

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Echocardiography was performed using a Vevo 2100 system (VisualSonics) to analyze cardiac function after MI and AngII/PE infusion. Systolic function was analyzed in conscious mice. Briefly, hair removal cream was applied to the chest to remove all fur. A layer of ultrasound gel was applied to the animal’s chest, and the probe was lowered at the parasternal line, 90° between the probe and the heart. B- and M-modes were performed to record 2-dimensional and 1-dimensional transverse cardiac measurements and used to analyze LV function. Independent echocardiography on mice 7 months post-induction at The Ohio State University also found no differences in cardiac measurements between control and fibroblast-ablated hearts.
Diastolic function was measured in mice anesthetized with 4% isoflurane in 100% oxygen. Anesthesia and body temperature were maintained at 1% isoflurane and 37°C, respectively. M-mode in the short axis at the level of papillary muscles was used to measure systolic function. The 4-chamber apical view with color and tissue Doppler mode was used to measure E/A and E/E’ ratios. All measurements were performed by an experienced operator blinded to the mouse genotypes.

Kuwabara J.T., Hara A., Bhutada S., Gojanovich G.S., Chen J., Hokutan K., Shettigar V., Lee A.Y., DeAngelo L.P., Heckl J.R., Jahansooz J.R., Tacdol D.K., Ziolo M.T., Apte S.S, & Tallquist M.D. (2022). Consequences of PDGFRα+ fibroblast reduction in adult murine hearts. eLife, 11, e69854.

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Echocardiography Analysis of Cardiac Function

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Echocardiography was used to evaluate cardiac hypertrophy, systolic and diastolic
function after virus injection for 3 weeks. A Visual sonics high-resolution Vevo 2100
system (VisualSonics Inc., Toronto, Canada) was used. In brief, mice were anesthetized
with 3.0% isoflurane (Airflow velocity: 1 l/min). After the pain reflex disappears and the
heart rate stabilized at 400 to 500 beats per min. Parasternal long-axis images were
acquired in B-mode with appropriate positioning of the scan head and the maximum LV length
identified. The M-mode cursor was positioned perpendicular to the maximum LV dimension in
end-diastole and systole, and M-mode images were obtained for measuring wall thickness and
chamber dimensions. Apical four-chamber view was acquired and the peak flow velocities
during early diastole (E wave) were measured across the mitral valve. Early-diastolic peak
velocity (E’ wave) of mitral valve ring was also measured in this view, then E/E’ which
reflected the left ventricle diastolic function were calculated.

Liu T., Yang F., Liu J., Zhang M., Sun J., Xiao Y., Xiao Z., Niu H., Ma R., Wang Y., Liu X, & Dong Y. (2019). Astragaloside IV reduces cardiomyocyte apoptosis in a murine model of coxsackievirus B3-induced viral myocarditis. Experimental Animals, 68(4), 549-558.

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Transverse Aortic Constriction Protocol

Cited 2 times

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Ten- to twelve-weeks-old male mice were subjected to TAC as described previously (67 (link)). Male mice were used for TAC because female C57Bl6/J mice have differential responses to TAC, that is beyond the scope of this limited study that focuses on biochemical contributions of myofilament proteins to baseline cardiac function (60 (link), 68 (link)). An intraperitoneal injection of ketamine (100 mg/kg) and xylazine (5 mg/kg) was administered to anesthetize mice. The thoracic aorta was visualized through an incision in the left chest at the second intercostal space. A 27-gauge needle was laid across the thoracic aorta, an overlying ligature was imposed, and the needle was then withdrawn, resulting in vascular stenosis.
Nonanesthetized, lightly restrained mice were subjected to echocardiography using a Vevo 2100 system (VisualSonics) equipped with an MS400C scanhead, as previously described (69 (link)). M-mode recordings were acquired from a short-axis view at the level of the papillary muscles. When the largest and smallest left ventricular cavity areas were present, the left ventricular internal diameter (LVID) was measured at both the end of diastole (LVIDd) and the end of systole (LVIDs). The formula for calculating fractional shortening was (LVIDd-LVIDs)/LVIDd x 100%. Averages for each parameter, recorded at least three times, are provided.

Lee E., May H., Kazmierczak K., Liang J., Nguyen N., Hill J.A., Gillette T.G., Szczesna-Cordary D, & Chang A.N. (2024). The MYPT2-regulated striated muscle-specific myosin light chain phosphatase limits cardiac myosin phosphorylation in vivo. The Journal of Biological Chemistry, 300(2), 105652.

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Echocardiography for Small Animal Imaging

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Transthoracic echocardiography was performed using a Vevo 2100 system (VisualSonics, Toronto, Canada). Animals were anesthetized with isoflurane (1.5–2%) and placed on a warming platform in a supine position. Two-dimensional short axis views were recorded at the mid-papillary level. Parasternal long axis views were recorded at the plane of the aortic valve with a concurrent visualization of the left-ventricular apex. Conventional measurements were obtained from B-mode recordings using a MS 400 transducer (center frequency 30 MHz) with a frame rate of 230–400 frames/s. Anterior and posterior wall thickness, left-ventricular diameter and the area of the left-ventricular cavity were recorded according to standard procedures. All images were recorded digitally, and off-line analysis was performed using the Vevo 2100 software.

Pecha S., Yorgan K., Röhl M., Geertz B., Hansen A., Weinberger F., Sehner S., Ehmke H., Reichenspurner H., Eschenhagen T, & Schwoerer A.P. (2019). Human iPS cell-derived engineered heart tissue does not affect ventricular arrhythmias in a guinea pig cryo-injury model. Scientific Reports, 9, 9831.

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Echocardiography Evaluation of Cardiac Function

Cited 1 time

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We used echocardiography with a VisualSonics Vevo 2100 system to evaluate heart function in WT, PHB2^Tg, and Pgam5^Cko mice. First, anesthetization was performed through 3.0% isoflurane. Then, parasternal long-axis images, M-mode images were used to measure myocardial structure and function based on previous studies. Echocardiographic images of the ascending and abdominal aorta were also obtained to quantify their maximum diameters 42 (link). All measurement data are the average of at least three consecutive cardiac cycles.

Cai C., Li Z., Zheng Z., Guo Z., Li Q., Deng S., Shi N., Ou Q., Zhou H., Guo Z., Chen Z, & Zhu H. (2023). Pgam5-mediated PHB2 dephosphorylation contributes to endotoxemia-induced myocardial dysfunction by inhibiting mitophagy and the mitochondrial unfolded protein response. International Journal of Biological Sciences, 19(14), 4657-4671.

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Ultrasound Analysis of Aortic Diameter

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Upon completion of Ang II infusion, the mice were anesthetized, and ultrasound analysis of the abdominal aorta was performed using a Vevo 2100 System (Visual Sonics, Canada) equipped with a linear array ultrasound transducer. The maximum aortic diameters of the mice were measured three times by an investigator who was blinded to the group information.

Wang L., Hu C., Dong Y., Dai F., Xu Y., Dai Y., Shao L, & Zhu D. (2021). Silencing IL12p35 Promotes Angiotensin II-Mediated Abdominal Aortic Aneurysm through Activating the STAT4 Pathway. Mediators of Inflammation, 2021, 9450843.

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Echocardiographic Evaluation of Mice

Cited 1 time

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Trans-thoracic echocardiography was performed on all mice by using a Vevo 2100 system with a MS400 linear array transducer (VisualSonics, ON, Canada) as previously reported (Zhang et al., 2018 (link)). Briefly, mice were anesthetized with 2% isoflurane and kept warm on a heated platform (37°C). The chest hairs were removed by using depilatory cream, and a layer of acoustic coupling gel was applied to the thorax. An average of 10 cardiac cycles of standard 2-D and m-mode short axis at mid-papillary muscle level were analyzed. Left ventricular ejection fraction and dimensions were calculated by using a modified Quinone method.

Bao Q., Zhang B., Suo Y., Liu C., Yang Q., Zhang K., Yuan M., Yuan M., Zhang Y, & Li G. (2020). Intermittent hypoxia mediated by TSP1 dependent on STAT3 induces cardiac fibroblast activation and cardiac fibrosis. eLife, 9, e49923.

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