Elisa reader

Manufactured by Thermo Fisher Scientific

Sourced in United States, Finland, China, Germany

The ELISA reader is a laboratory instrument used to measure the absorbance of light in ELISA (Enzyme-Linked Immunosorbent Assay) plates. It is designed to quantify the amount of a specific substance, such as a protein or an antibody, present in a sample by detecting the color change produced by an enzyme-catalyzed reaction.

Automatically generated - may contain errors

Lab products found in correlation

Methyl thiazolyl tetrazolium (mtt), by Merck Group (5 mentions) Dimethyl sulfoxide (dmso), by Merck Group (4 mentions) Methyl thiazolyl tetrazolium (mtt), by Thermo Fisher Scientific (3 mentions) Mtt solution, by Merck Group (2 mentions) Maxisorp, by Thermo Fisher Scientific (2 mentions) Goat anti mouse secondary antibody, by Abcam (2 mentions) Mouse anti his mab, by Tiangen Biotech (2 mentions) Hrp conjugated goat anti mouse igg, by BD (2 mentions) Crystal violet staining solution, by Merck Group (2 mentions) Cell counting kit 8 (cck8), by Dojindo Laboratories (2 mentions) Alizarin red, by Merck Group (2 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions) Alpha tubulin, by Merck Group (1 mentions) Western lightning ecl, by PerkinElmer (1 mentions) Sigmafast protease inhibitor cocktail, by Merck Group (1 mentions) Poinceau, by Merck Group (1 mentions) Human fgf basic quantikine elisa kit, by R&D Systems (1 mentions) Chemostar imager, by Intas (1 mentions) Apo one homogeneous caspase 3 7 assay, by Promega (1 mentions) Etoposide, by Merck Group (1 mentions)

Close spelling variants of this product

ELISAs (35 citations) Multiskan FC ELISA reader (11 citations) Automated ELISA reader (9 citations) Multiskan Ascent ELISA reader (7 citations) Multiskan MK3 ELISA reader (7 citations) MK3 ELISA reader (5 citations) Elisa Reader spectrophotometer (4 citations) Multiscan EX ELISA plate reader (4 citations) Multiscan-Ascent ELISA reader (3 citations) Multiscan ELISA reader (3 citations)

167 protocols using elisa reader

CASP Activity Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

CASP 3 activity in living cells was tested with the GreenNuc™ CASP -3 Activity Kit (Beyotime, China) according to the manufacturer’s instructions. H292 or EA.hy926 (2 × 10⁴ cells per well) cultured in 96-well plates were transfected with 0.5 μg of empty plasmid or plasmids expressing S-flag, N-flag, M-flag or N-flag, respectively, for 48 h. Then, cells were incubated with 200 μl PBS containing 10 μM active CASP 3 detection reagents for 30 min at room temperature. The fluorescent intensity of CASP 3 activity was quantified using the ELISA reader (Thermo Fisher Scientific, USA) at the absorption of 485 nm (excitation wavelength).
CASP 8 (Beyotime, China), CASP 9 (Beyotime, China), and CASP 12 (SLF Biotech, China) activities were measured using the CASP Activity Kit according to the manufacturer’s instructions. Briefly, transfected (2 μg of mock or M-flag) of H292 (1 × 10⁶ well) were scraped and lysates were left on ice for 5 min. The buffer containing the substrate peptides coupling p-nitroanilide (pNA) was added to the supernatant. The signal of pNA or CASP activity was detected at the wavelength of 405 nm by an ELISA reader (Thermo Fisher Scientific, USA).

Yang Y., Wu Y., Meng X., Wang Z., Younis M., Liu Y., Wang P, & Huang X. (2022). SARS-CoV-2 membrane protein causes the mitochondrial apoptosis and pulmonary edema via targeting BOK. Cell Death and Differentiation, 29(7), 1395-1408.

+ Open protocol

+ Expand

Cytotoxicity and Membrane Integrity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells (1 × 104 cells per well) were plated in 96‐well plates, and MTT was added to cell cultures at a final concentration of 5 mg/mL 4 hours at 37°C. DMEM was removed, and DMSO was added into cell for 20 minutes at 37°C. Absorbance was measured at 570 nm using an ELISA reader (Thermo Labsystems).
After transfection of 48 hours, cell was used to measure the LDH activity levels using LDH activity kits. Absorbance was measured at 450 nm using an ELISA reader (Thermo Labsystems).

Qiu K., Xie Q., Jiang S, & Lin T. (2019). miR‐98‐5p promotes apoptosis and inhibits migration and cell growth in papillary thyroid carcinoma through Bax/Caspase‐3 by HMGA2. Journal of Clinical Laboratory Analysis, 34(2), e23044.

+ Open protocol

+ Expand

Determining Curcumin Non-Cytotoxic Concentration

Check if the same lab product or an alternative is used in the 5 most similar protocols

To determine the non-cytotoxic concentration of curcumin, PC12 cell viability was assessed by MTT assay. A quantity of 10 × 10³ PC12 cells was cultured in 96-well plates coated with poly-L-lysine and exposed to different concentrations of SPIONs, ranging from 10 to 100 µg/mL and 10–100 μM for curcumin for one, two, and three days. At the mentioned times cells were incubated with MTT solution (0.5 mg/mL) (Sigma Aldrich Co., St. Louis, MO, USA) for 4 h, and absorbance was measured by the ELISA reader at 560 nm (Thermo LabSystems Inc, Beverley, MA, USA). The MTT assay was performed according to the method of Mosmann, and absorbance was measured by the ELISA reader at 560 nm (Thermo LabSystems Inc, Beverley, MA, USA).

Zarei M., Esmaeili A., Zarrabi A, & Zarepour A. (2022). Superparamagnetic Iron Oxide Nanoparticles and Curcumin Equally Promote Neuronal Branching Morphogenesis in the Absence of Nerve Growth Factor in PC12 Cells. Pharmaceutics, 14(12), 2692.

+ Open protocol

+ Expand

Antioxidant and Oxidative Stress Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

With protease inhibitor cocktail, liver and heart tissue homogenates were prepared from all groups of animals for assessment of enzymatic and nonenzymatic antioxidant related to oxidative stress. [3] (link)[4] [5] Hepatocytes and cardiomyocytes were also isolated using a two-step collagenase type II digestion perfusion technique. 7, 8 Estimation of lipid peroxidation and nitric oxide (NO)
TBA test was used to assess the formation of thiobarbituric acid reactive substance (TBARS) as a result of lipid peroxidation, and the absorbance was measured at 530 nm using an ELISA Reader (Thermo Scientific). 9 Using the molar extinction coefficient (1.56×10 5 cm 2 /mM), the results were represented as nmoles of TBARS per milligram of protein. In aerated fluids, NO decomposes quickly to generate stable nitrite/nitrate compounds. Previously reported method used the Griess reaction to estimate nitrite buildup, and the absorbance was measured at 550 nm using an ELISA Reader (Thermo Scientific). 10 NO was measured as µmole/mg of protein in the sample.

Banerjee A., Mukherjee S, & Maji B.K. (2021). Monosodium glutamate causes hepato-cardiac derangement in male rats. Human & experimental toxicology, 40(12_suppl).

+ Open protocol

+ Expand

Evaluation of A431 Cell Viability

Check if the same lab product or an alternative is used in the 5 most similar protocols

A431 cells were cultured in EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids + 10% Fetal Bovine Serum at 37 °C under 5% CO₂ in air. The cells were collected by trypsinization and placed onto a 10 cm tissue culture Petri dish, then allowed to grow for 2–4 days. For MTT assay, cells (5 × 10³ per well in a 96-well culture plate) were incubated overnight and then treated with material-Ab_EGFR for 24 h incubation in an incubator (37 °C). Cell viability was evaluated using MTT assay. A431 cells were seeded into the 96-well culture dish plates contain 150 μL of the culture medium for 24 h. Before the assay, the materials were mixed into the culture medium at concentrations ranging from 0 to 50 μg mL⁻¹. After 24 h, 150 μL of MTT solution (1 mg 150 mg mL⁻¹) was added for 4 h reaction with the cells at 37 °C. After removal of the medium and MTT solution, 150 μL of DMSO was added to each well, and the assay plate was read at optical density 595 nm with an ELISA reader (Thermo Electron, Waltham, MA, USA). The absorbance of the untreated cells in the control group was considered 100%.

Kuo W.S., Chang C.Y., Huang K.S., Liu J.C., Shao Y.T., Yang C.H, & Wu P.C. (2020). Amino-Functionalized Nitrogen-Doped Graphene-Quantum-Dot-Based Nanomaterials with Nitrogen and Amino-Functionalized Group Content Dependence for Highly Efficient Two-Photon Bioimaging. International Journal of Molecular Sciences, 21(8), 2939.

+ Open protocol

+ Expand

Western Blot Analysis and FGF2 ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blots were generated by the semi-dry method. Proteins obtained from cell line lysates using SIGMAFast protease inhibitor cocktail (Sigma) were transferred onto nitrocellulose membranes (Bio-Rad, München, Germany) and blocked with 5% dry milk powder dissolved in phosphate-buffered-saline buffer (PBS). The following antibodies were used: alpha-Tubulin (Sigma), OTX1 (Santa Cruz Biotechnology, Heidelberg, Germany), OTX2 (Abcam, Cambridge, UK), and ZHX1 (Santa Cruz Biotechnology). For loading control the blots were stained with Poinceau (Sigma) and then detection of alpha-Tubulin (TUBA) was performed. Secondary antibodies were linked to peroxidase for detection by Western-Lightning-ECL (Perkin Elmer, Waltham, MA, USA). Documentation was performed using the digital system ChemoStar Imager (INTAS, Göttingen, Germany).
Quantification of FGF2 protein in cell culture supernatants was performed as follows: log-phase cells were washed twice in PBS and cultivated in fresh medium at 1x10⁶ cells per ml and supernatants harvested after 24 h. ELISA was performed using the Human FGF basic Quantikine ELISA Kit (R&D Systems) and an ELISA reader (Thermo Electron, Vantaa, Finland).

Nagel S., Ehrentraut S., Meyer C., Kaufmann M., Drexler H.G, & MacLeod R.A. (2015). Aberrantly Expressed OTX Homeobox Genes Deregulate B-Cell Differentiation in Hodgkin Lymphoma. PLoS ONE, 10(9), e0138416.

+ Open protocol

+ Expand

Evaluating miR-130a Inhibitor's Impact on SKNO-1 Cell Viability and Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell line SKNO-1 was plated at a concentration of 10,000 cells per well in triplicate for each condition in a 96-well plate 24 h after electroporation with the miR-130a inhibitor or the control miRNA inhibitor (Qiagen). Etoposide (5 μM, Sigma, München, Germany) or the same volume of DMSO (Sigma) (mock treatment) was added to each well. After 24 h, MTT (5 μg/mL, Sigma) was added to each well. After a 3-h incubation in 37 °C, reactions were stopped by adding DMSO/isopropanol (2:1). Absorbance was determined at 570 nm by an ELISA reader (Thermo Electron, Vantaa, Finland). For apoptosis, caspase 3/7 activity was detected using the ApoONE Homogeneous Caspase 3/7 Assay (Promega) following the manufacturer’s instruction. Fluorescence was measured by a spectrofluorometer instrument (Perkin Elmer, Waltham, MA, USA). The cell viability and apoptosis assays were performed in triplicate.

Ding C., Chen S.N., Macleod R.A., Drexler H.G., Nagel S., Wu D.P., Sun A.N, & Dai H.P. (2018). MiR-130a is aberrantly overexpressed in adult acute myeloid leukemia with t(8;21) and its suppression induces AML cell death. Upsala Journal of Medical Sciences, 123(1), 19-27.

+ Open protocol

+ Expand

Cell Viability Assay with Cigarette Smoke Extract

Check if the same lab product or an alternative is used in the 5 most similar protocols

MTT assays were carried out on different cell passaged to assess the cell viability under the stress due to CSE treatment at various concentrations (0.5, 1, 2, and 4%). One hundred percent CSE solution prepared in previous step was diluted in treating samples to get the final concentrations. Cells were grown in 96 wells plate with the density of 3×10³ cell/well and exposed to CSE solution within 24 hours. Three replicates were performed for each concentration. The absorbance of colored solution was quantified by measuring at 560 nm wavelength using Elisa reader (Thermo Electron Co., Shanghai, China) [9 (link)].

Le V., Kim Y.H, & Min J. (2014). The dependence of nitric oxide synthase inhibition caused by cigarette smoking extract on the cellular aging of bovine aortic endothelial cells. Environmental Health and Toxicology, 29, e2014010.

+ Open protocol

+ Expand

Radioresistance of Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The tumor cell lines, HepG₂, EC-9706 and MCF-7, purchased from the Cell Culture Centre of the Chinese Academy of Medical Science nad Peking Union Medical College (Beijing, China) and were conserved by the Radiation Hazard Evaluation Laboratory of the Chinese Academy of Medical Science and Peking Union Medical College (Tianjin, China). Cells were maintained in RPMI-1640 as suspensions at densities of 1×10⁵/ml and then irradiated by a ¹³⁷Cs γ-ray with a dose rate of 1.23 Gy/min and dose points of 0, 1, 2, 4 and 8 Gy. Following inoculation in 96-well plates with 200 μl of each irradiation dose (six copies) per well, cells of the irradiated group and a non-irradiated group as control were cultured at 37°C in humidified conditions at 5% CO₂ for three days. Then, 20 μl MTT at a concentration of 5 mg/ml was added to each well and co-incubated for 4 h. Following the removal of raffinate, 150 μl dimethyl sulfoxide was added to every well and the absorption value (A) was evaluated by an ELISA reader (Thermo Electron Corporation, Waltham, MA, USA) at a wavelength of 492 nm. The surviving fraction (SF) was calculated using the following formula:

SF = A_{irradiated group} / A_{non - irradiated group}

LI J., WANG Y., DU L., XU C., CAO J., WANG Q., LIU Q, & FAN F. (2014). Nested PCR for mtDNA-4977-bp deletion and comet assay for DNA damage - a combined method for radiosensitivity evaluation of tumor cells. Oncology Letters, 7(4), 1083-1087.

+ Open protocol

+ Expand

Quantifying Immune Markers by ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols

For quantification of Ym1 levels, a commercially available ELISA kit was used according to the manufacturer’s protocol (R&D Systems, Minneapolis, MN, USA). The serum levels of OVA-specific IgG1 and IgE were also measured by ELISA. Briefly, the 96-well plates were coated for overnight reaction at 4°C with OVA (5 μg/ml) in PBS. After 1% bovine serum albumin (BSA) blocking and washing, diluted serum samples were added to the plates with 2-hour incubation at room temperature. Then, rat anti-mouse IgE-biotin (SouthernBiotech) or rat anti-mouse IgG1-biotin (Bio-Rad) antibodies were incubated, followed by streptavidin–horseradish peroxidase (HRP) incubation. Last, ABTS (Roche) was added as a substrate, and reactions were read at 405 nm using an ELISA reader (Thermo Electron Corporation). IL-6, IL-12, and IL-10 levels from the BMM culture supernatant were determined by ELISA using their capture antibodies and biotin-labeled detection antibodies (IL-6 and IL-12, eBioscience; IL-10, R&D Systems). Binding of antibodies was detected by europium-labeled streptavidin using a dissociation-enhanced lanthanide fluoroimmunoassay system (Wallac).

Zhu W., Lönnblom E., Förster M., Johannesson M., Tao P., Meng L., Lu S, & Holmdahl R. (2020). Natural polymorphism of Ym1 regulates pneumonitis through alternative activation of macrophages. Science Advances, 6(43), eaba9337.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!