Log In Sign up
The largest database of trusted experimental protocols

Luria bertani medium

Manufactured by HiMedia
Visit Supplier
Sourced in India

Luria Bertani (LB) medium is a commonly used growth medium for bacterial cultures. It provides the necessary nutrients and conditions for the cultivation of a wide range of bacterial species. The LB medium contains a complex blend of peptides, yeast extract, and salts that support bacterial growth and proliferation.

Automatically generated - may contain errors

Lab products found in correlation

Imidazole, by Merck Group (2 mentions) T4 dna ligase, by Thermo Fisher Scientific (2 mentions) Duroquinone, by Merck Group (1 mentions) N dodecyl β d maltoside, by Merck Group (1 mentions) Octyl β d glucopyranoside, by Merck Group (1 mentions) 50 bp dna ladder, by Thermo Fisher Scientific (1 mentions) Bicinchoninic acid bca protein estimation kit, by Thermo Fisher Scientific (1 mentions) Agar agar, by HiMedia (1 mentions) Sodium dithionite, by Merck Group (1 mentions) Coomassie brilliant blue r 250, by Merck Group (1 mentions) Verso cdna synthesis kit, by Thermo Fisher Scientific (1 mentions) Tris base, by Merck Group (1 mentions) Trizol reagent, by Merck Group (1 mentions) Sodium pyruvate, by Merck Group (1 mentions) Restriction endonuclease, by New England Biolabs (1 mentions) Kanamycin, by HiMedia (1 mentions) Amine coupling kit, by Cytiva (1 mentions) Cm5 sensor chip, by Cytiva (1 mentions) Surfactant p20, by Cytiva (1 mentions) Phosphate buffered saline (pbs), by Cytiva (1 mentions)

14 protocols using luria bertani medium

1

Purification and Analysis of a Recombinant Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sodium pyruvate, tris base, imidazole, duroquinone, diethyl ether, sodium dithionite, n-dodecyl β-D maltoside (DDM), octyl-β-D glucopyranoside (OG), isopropyl β-D-1-thiogalactopyranoside (IPTG), phenylmethylsulfonyl fluoride (PMSF), n-PG, SHAM, coomassie brilliant blue R-250, and TRIzol reagent were purchased from Sigma Aldrich, St Louis, Mo, USA. The protease inhibitor ‘cocktail' was purchased from Roche. CM5 sensor chips, PBS, surfactant P20, glycine-HCl pH 2.5, sodium acetate pH 5, and amine coupling kit were purchased from GE Healthcare Bio-Sciences Corp., USA. Verso cDNA synthesis kit, 50 bp DNA ladder, bicinchoninic acid (BCA) protein estimation kit, protein molecular mass standard, and 3 kDa cut-off snakeskin dialysis membrane were purchased from Thermo Fisher Scientific, USA. Talon cobalt metal affinity resin was purchased from Clontech, Takara Bio, Japan. His-Tag mouse monoclonal antibody (HRP conjugated) was purchased from Cell Signaling Technologies. ECL Western blotting reagents were purchased from GE Healthcare Bio-Sciences Corp., USA. Polymerase chain reaction (PCR) components and restriction endonucleases were purchased from New England Biolabs. Luria-Bertani medium, agar-agar, and kanamycin were purchased from Hi-Media, Mumbai, India. Ferrous sulfate and glycerol were purchased from SRL. All the compounds used were of the biochemical grade.
Sankar T.V., Saharay M., Santhosh D., Vishwakarma A, & Padmasree K. (2022). Structural and Biophysical Characterization of Purified Recombinant Arabidopsis thaliana's Alternative Oxidase 1A (rAtAOX1A): Interaction With Inhibitor(s) and Activator. Frontiers in Plant Science, 13, 871208.
+ Open protocol
+ Expand
2

Overexpression and Purification of MalZ Enzyme

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasmid PCS19 containing the malZ gene was a generous gift from Prof. Winfried Boos, (University of Konstanz, Germany). The plasmid contains malZ gene under control of a strong IPTG inducible T5 promoter and it contains ampicillin resistance for selection and imparts a C-terminal 6x His tag to the protein. E.coli BL-21 cells were transformed with the plasmid and grown at 37°C in Luria Bertani medium (HiMedia, Mumbai, India) containing 80µg/ml ampicillin (HiMedia, Mumbai, India). Induction for protein overexpression was done with 1mM IPTG (SRL, Mumbai, India) when O.D. at 600nm reached to a value of 0.6. The culture was kept for 8 hours post induction and protein expression was confirmed on a SDS PAGE gel. MalZ purification was carried out by Nickel affinity chromatography on a 5ml His-Trap column and Akta Purifier (GE Healthcare, Little Chalfont, U.K.) was used for the purification process. 20mM sodium phosphate buffer (Merck, Darmstadt, Germany) containing 500mM NaCl (Merck, Darmstadt, Germany) were used for lysis and column equilibration. MalZ was eluted near 200mM imidazole (Sigma Aldrich, Missouri, U.S.A.) through a linear gradient of imidazole from 20mM to 500mM.
Pastor A., Singh A.K., Fisher M.T, & Chaudhuri T.K. (2016). Protein folding on Biosensor tips: Folding of Maltodextrin glucosidase monitored by its interactions with GroEL. The FEBS journal, 283(16), 3103-3114.
+ Open protocol
+ Expand
3

Cultivation and Characterization of B. methylotrophicus

Check if the same lab product or an alternative is used in the 5 most similar protocols
Luria-Bertani medium (HiMedia®, Mumbai, India) supplemented with glucose (LBG broth) was used for cultivation of B. methylotrophicus BM47. LBG agar medium (Laboratorios Conda S.A., Madrid, Spain) was used for cultivation of the test bacteria and yeasts, as well as for implementation of agar well diffusion assay. Malt agar (HiMedia®) was used for cultivation/maintenance of the test fungi. The culture media were prepared according to the typical formula (8 , 9 ).
Tumbarski Y., Deseva I., Mihaylova D., Stoyanova M., Krastev L., Nikolova R., Yanakieva V, & Ivanov I. (2018). Isolation, Characterization and Amino Acid Composition of a Bacteriocin Produced by Bacillus methylotrophicus Strain BM47. Food Technology and Biotechnology, 56(4), 546-552.
+ Open protocol
+ Expand
4

Microbial Culture Preparation Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Difco sporulation medium (DSM) and Luria-Bertani medium (LB) were purchased from Himedia, India. Methanol 99.5%, phosphate-buffered saline (pH 7.4), and chloroform 99.5% were purchased from Merck, Germany. F-primer f27, R-primer r1492, DNA master mix, and PCR water were purchased from Phusa biochem LTD. Company.
Hong L.B., Nguyen N.H., Thanh D.N.L., & Nguyen M.C. (2021). Isolation and screening of carotenoid-producing Bacillus spp. from seashore saline soil and seawater at Hon Son islet. Can Tho University Journal of Science, 13(1).
+ Open protocol
+ Expand
5

Calcium Hydroxide and Phosphoric Acid Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Calcium hydroxide [Ca(OH)2], phosphoric acid (H3PO4), and Luria–Bertani (LB) medium were purchased from Himedia Co. (Mumbai, India), and the reagents used in this study (99.9% purity) were purchased from Sigma Aldrich (St. Louis, MO, USA).
MubarakAli D., Arunachalam K., Lakshmanan M., Badar B., Kim J.W, & Lee S.Y. (2023). Unveiling the Anti-Biofilm Property of Hydroxyapatite on Pseudomonas aeruginosa: Synthesis and Strategy. Pharmaceutics, 15(2), 463.
+ Open protocol
+ Expand
6

Probiotic E. coli Nissle 1917 in NASH Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
The probiotic Escherichia coli Nissle 1917 strain (Mutaflor®, Ardeypharm GmbH, Herdecke, Germany) was grown on Luria Bertani (LB) medium (HiMedia, Mumbai, India). The number of viable bacterial cells in one mL was determined using the plate counting method and calculated as Colony-forming units per mL (CFU/ mL) = no. of colonies × dilution factor [35 ]. A dose of 1 × 108 CFU/mL was administered daily by gavage to treated animals [36 (link)–39 (link)]. Cholesterol and bile salts were purchased from Ralin BV (Lijinbaan, Netherlands) (Catalogue number 81254). LB broth was purchased from HiMedia (Mumbai, India, Catalogue No. M1245).
NASH was induced by feeding rats HFHSD consisting of 68.75% standard chow, 20% lard, 10% sucrose, 1% cholesterol, and 0.25% bile salts [40 (link)]. Components were mixed using water, made into pellets and left to dry. The diet was prepared every five days and stored in the refrigerator.
Hany N.M., Eissa S., Basyouni M., Hasanin A.H., Aboul-Ela Y.M., Elmagd N.M., Montasser I.F., Ali M.A., Skipp P.J, & Matboli M. (2022). Modulation of hepatic stellate cells by Mutaflor® probiotic in non-alcoholic fatty liver disease management. Journal of Translational Medicine, 20, 342.
+ Open protocol
+ Expand
7

Expression and Characterization of Cellulase from E. coli

Check if the same lab product or an alternative is used in the 5 most similar protocols
E. coli DH5α and E. coli BL21 (DE3) were used for plasmid replication and expression in host cells, respectively. Both these strains were procured from Invitrogen (CA, USA). The Luria–Bertani (LB) medium purchased from Hi-Media (Mumbai, India) was used for maintaining the strains. The pET-28a vector (Invitrogen, USA) was used as a cloning and expression vector. Restriction enzymes and the DNA ligation kit were procured from New England Biolabs (MA, USA). For determining the enzyme activity, all the substrates were bought from Sigma (USA). Cellic CTec2 was supplied by Novozymes (Bagsværd, Denmark). All other reagents used were of analytical grade and purchased from Sigma (St. Louis, USA), Merck (Darmstadt, Germany), Fisher Scientific (Massachusetts, USA), CDH (India) and SRL (India) and Hi-Media (Mumbai, India). Sugarcane bagasse used in the study was collected from a local juice shop.
Dadwal A., Sharma S, & Satyanarayana T. (2021). Recombinant cellobiohydrolase of Myceliophthora thermophila: characterization and applicability in cellulose saccharification. AMB Express, 11, 148.
+ Open protocol
+ Expand
8

Characterization of Pseudomonas Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols
All the chemicals and reagents used throughout the study were of analytical grade. Luria Bertani (LB) medium, Pseudomonas broth, TTC (2,3,5-TTC - Triphenyltetrazolium chloride), crystal violet dye, and glutaraldehyde solution was procured from Hi-media, Mumbai. Azocasein and Elastin Congo red was procured from Sigma-Aldrich Pvt Ltd. All the solvents Methanol, Chloroform, n-hexane, Ethyl acetate, Ethanol, and HCL used in the study were of 99.9% purity obtained from Thermo Fisher Scientific India Pvt Ltd., Mumbai, India.
S.a.m.r.e.e.n., Qais F.A, & Ahmad I. (2022). Anti-quorum sensing and biofilm inhibitory effect of some medicinal plants against gram-negative bacterial pathogens: in vitro and in silico investigations. Heliyon, 8(10), e11113.
+ Open protocol
+ Expand
9

Cultivation of S. equisimilis and E. coli

Check if the same lab product or an alternative is used in the 5 most similar protocols
S. equisimilis was cultivated in Trypton soya broth (TSB) medium (HiMedia, Mumbai, India). E. coli Top10F’ strain was cultivated in Luria-Bertani (LB) medium (HiMedia, Mumbai, India) with an appropriate concentration of antibiotic (Apramycin) 50 mg/L. The two clones obtained have similar genome editing, and one particular clone is taken into the study.
Chaudhari A.M., Vyas S., Singh V., Patel A., Joshi C, & Joshi M.N. (2022). CRISPR-Cas9 Mediated Knockout of SagD Gene for Overexpression of Streptokinase in Streptococcus equisimilis. Microorganisms, 10(3), 635.
+ Open protocol
+ Expand
10

Synthesis and Characterization of Zinc Phthalocyanine

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hydrochloric acid (HCl) (37%), hydrogen peroxide (H2O2) (50%), sodium hydroxide (NaOH), sodium chloride (NaCl), potassium permanganate (KMnO4), sulfuric acid (H2SO4) (98%), 125 mm filter paper (grade-1), acetone (CH3COCH3) and ortho-phosphoric acid (H3PO4) (84%) were obtained from Merck (India). Phosphorous pentoxide (P2O5), sodium dodecyl sulfate (SDS) and hydrazine monohydrate (N2H4·H2O) (98%) were obtained from Sigma-Aldrich (India). Zinc phthalocyanine (ZnPc) (MW = 577.91 g mol−1; dye content 97%) was purchased from Sigma-Aldrich (Germany). Graphite flakes (99.99%) were obtained from Alfa Aesar (India). Indium tin oxide (ITO)-coated glass substrates were procured from Macwin (India). The ITO-coated glass substrates were cut into 1 × 1 cm2 pieces and served as the substrate for the ZnPc deposits. Luria–Bertani (LB) medium was purchased from Himedia (India). Parafilm wax paper was obtained from Tarsons (India). The aforementioned chemicals were of analytical grade and were used without further purification. Milli-Q grade water was used in the experiments.
Das N.M., Singh A.K., Ghosh D, & Bandyopadhyay D. (2019). Graphene oxide nanohybrids for electron transfer-mediated antimicrobial activity. Nanoscale Advances, 1(9), 3727-3740.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!