Gastrodin (pure: ≥ 98%) was presented by Kunming Pharmaceutical Group Co.; aspirin was purchased from Ogina Pharmaceutical Co. (Shen Yang, China); rat COX-1 and COX-2 ELISA Kits, rat CK-MB and cTnT ELISA Kits, rat TNF-α and IL-6 ELISA Kits were provided by Cusabio Biotech Co. (Wu Han, China); BCA Protein Assay Kit, Hematoxylin & Eosin (H&E) Staining Kit and TTC solution were provided by Beyotime Biotechnology Co. (Shang Hai, China; Cat. No.: P0010, C0105M); O.C.T. Tissue-Tek was provided by Kang cheng Biological Co. (Shang Hai, China; Cat. No.: 4583); COX-1, COX-2, NLRP3, ASC, caspase-1 and GSDMD-N monoclonal Antibodies were obtained from Affinity Biosciences Co. (Jiang Su, China; Cat. No.: AF7002 and AF7003); GAPDH and β-actin monoclonal Antibodies were obtained from Santa Cruz Co. (Santa Cruz, America; Cat. No.: sc-47724); Fluorescent monoclonal Antibodies from Mouse were purchased from Proteintech Group Co. (Wu Han, China; Cat. No.: CL488-66061); DAPI and polyvinylidene fluoride membrane were obtained from Beyotime Biotechnology Co. (Shang Hai, China). All other experimental supplies were purchased from Sigma.
Hematoxylin eosin staining kit
Manufactured by Beyotime
Sourced in China, United States
The Hematoxylin-eosin staining kit is a laboratory product used for the staining and visualization of cellular structures in histological samples. The kit contains the necessary dyes and reagents to perform this standard staining technique, which is commonly used in pathology and histology applications.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Beyotime (3 mentions)
Anti bax, by Abcam (2 mentions)
Anti il 10, by Abcam (2 mentions)
Anti bcl 2, by Abcam (2 mentions)
Anti il 1β, by Abcam (2 mentions)
Anti β tubulin, by Abcam (2 mentions)
Anti inos, by Abcam (2 mentions)
β actin, by Beyotime (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Immunohistochemistry kit, by ZSGB-BIO (2 mentions)
Polyvinylidene fluoride membrane, by Beyotime (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Rat tnf α and il 6 elisa kits, by Cusabio (1 mentions)
Ttc solution, by Beyotime (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
Fluorescein isothiocyanate (fitc), by Beyotime (1 mentions)
Anti caspase 3, by Abcam (1 mentions)
Nissl staining solution, by Beyotime (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Lipopolysaccharides (lps), by Thermo Fisher Scientific (1 mentions)
31 protocols using hematoxylin eosin staining kit
1
Gastrodin Alleviates Inflammatory Injury
2
Neuroinflammation and Apoptosis Assay
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Naloxone was purchased from Taizhou Hongyao Chemical Co., Ltd. Naltrexone and phloroglucinol were supplied by Aladdin. The dialysis bags, FITC, fura-2AM fluorescent probe, nissl staining solution, hematoxylin eosin (HE) staining kit and BCA protein concentration determination kit were ordred from Beyotime Biotechnology . LPS, Fetal bovine serum (FBS), DMEM, penicillin-streptomycin (PS) and trypsin-EDTA (0.25%) were purchased from Gibco Company. Protein lysate (RIPA), phenylmethanesulfonyl fluoride (PMSF) and 4′,6-diamidino-2-phenylindole (DAPI) were bought from Beijing Solarbio Science & Technology Co., Ltd. Anti-CD11b, anti-F4/80, anti-iNOS, anti-TNF-α, anti-IL-1β, anti-IL-6, anti-IL-10, anti-Bax, anti-caspase 3, anti-bcl-2, anti-β-tubulin, anti-GAPDH, anti-β-actin were supplied by Abcam.
3
Physiological and Biochemical Assays for Immunomodulation
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LP (≥98%, 20 mg) was purchased from Solarbio (Bejing, China), and CYC (1 g) from Supelco (Sigma-Aldrich, St. Louis, MO, USA). The kits of total superoxide dismutase (SOD) activity, lipid oxidation (MDA), nitric oxide (NO), BCA protein concentration, and glutathione (GSH) were purchased from Beyotime (Shanghai, China). The sIgA ELISA kit was purchased from Elabscience Biotechnology (Wuhan, China). The interleukin (IL)-4, IL-12, interferon (IFN)-γ, IL-1β, IL-6, and tumor necrosis factor (TNF)-α ELISA kits were purchased from Beyotime (Shanghai, China). TLR4, MyD88, TRAF6, TRIF, p-P38 MAPK (P38), P38, p-NF-κB p65, NF-κB p65, β-actin, and horseradish peroxidase-conjugated secondary antibodies were purchased from Beyotime (Shanghai, China). The hematoxylin-eosin (HE) staining kit was purchased from Beyotime (Shanghai, China). All other chemicals were of the analytically purest grade available.
4
Histopathological Analysis of Liver
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Liver tissues were fixed in 4% paraformaldehyde and embedded in paraffin. Paraffin sections (5 μm) were then dewaxed and hydrated. Subsequently, the sections were sequentially stained with hematoxylin and eosin using a hematoxylin-eosin (HE) staining kit (Beyotime). Masson’s trichrome staining was performed to assess liver fibrosis, and sections were stained with Masson’s trichrome stain kit (Solarbio) according to the manufacturer’s instructions. For TUNEL staining, the sections were incubated with Biotin-dUTP, followed by staining with Streptavidin-HRP using the Colorimetric TUNEL Apoptosis Assay Kit (Beyotime). DAB was used to stain cell nuclei. Finally, the sections were examined under an optical microscope.
5
Histological and Apoptotic Analysis of Myocardial Tissue
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Paraffin sections (5 μm) of myocardial tissues were subjected to dewaxing and hydration. Then, the sections were stained with hematoxylin and eosin sequentially using Hematoxylin-Eosin (HE) Staining Kit (Beyotime, Shanghai, China). For detection of apoptotic cells in myocardial tissues, the paraffin sections were stained with TUNEL Apoptosis Assay Kit (Beyotime, Shanghai, China). The nucleus was stained with DAPI (Beyotime, Shanghai, China). Finally, the pathological changes and apoptotic cells in myocardial tissues were observed under optical or fluorescence microscope.
6
Histological Analysis of Spinal Cord
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Spinal cords centered on T10 (n = 6) were taken out (1 cm tissue segments) and fixed for 24 h in paraformaldehyde solution (Sinopharm Chemical Reagent Co., Ltd, Shanghai, China) (4% in 0.1 M PBS (Solarbio, Cat# P1010, Beijing, China) at 4°C). Then, tissues were dehydrated by graded ethanol. Finally, the tissues were embedded in OTC (Sakura Finetek, USA), and cut into 15-um-thick sections by slicer (Leica, Wetzlar, Germany). Tissue sections were stained with Hematoxylin-Eosin (HE) staining Kit (Beyotime, Cat# C0105S, Shanghai, China) and the Nissl Staining Solution (Meilunbio, Dalian, Cat# MA0129, Liaoning, China). The images were captured on Nikon confocal microscope (Nikon Instruments, Inc., Japan).
7
Monkfish Roe Alleviates Cyclophosphamide-Induced Toxicity
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Monkfish roe was purchased from Zhoushan International Aquatic Center (Zhejiang, China). Cyclophosphamide (CTX) was purchased from Aladdin (Shanghai, China). The hematoxylin-eosin (H&E) staining kit was purchased from Beyotime (Shanghai, China). Tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β ELISA kits were purchased from Boster (Wuhan, China). Immunoglobulin (Ig)A, IgM, and IgG ELISA kits were purchased from Yilai Ruite Biotechnology (Wuhan, China). Commercial kits for malondialdehyde (MDA), total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) were purchased from Jiancheng (Nanjing, China). Antibodies were purchased from Beyotime (Shanghai, China) or Cell Signaling Technology (Boston, MA, USA).
8
Hematoxylin-Eosin Tissue Staining
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Tissue sections were stained with hematoxylin–eosin (HE) staining Kit (Beyotime, China). The images were captured on an inverted fluorescence microscope (Leica, Germany).
9
Neuroprotective Mechanisms of Novel Compounds
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Tetraethyl orthosilicate (TEOS), potassium permanganate (KMnO4), sodium carbonate (Na2CO3), ethanol, methanol, acetone, and paraformaldehyde were purchased from Sinopharm Holding Chemical Reagent Company, Ltd. (Shanghai, China). Dimethyl sulfoxide (DMSO), phloretin, carboxymethylcellulose sodium, and 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT) were obtained from Aladdin Chemical Reagent Co. ROS assay kit, malondialdehyde (MDA) content assay kit, superoxide dismutase (SOD) content assay kit, total glutathione (GSH-Px) content assay kit, and Hematoxylin-Eosin/HE staining kit were bought from Beyotime Biotechnology Co. Anti-HIF-1α, Anti-iNOS, anti-cox-2, anti-IL-1β, anti-IL-10, anti-bax, anti-bcl-2, anti-Cl caspase-3, anti-β-Tubulin, and anti-actin were purchased from Abcam. PC12 cells and BV2 cells were obtained from the cell bank of Chinese Academy of Sciences.
10
Rat Cell Viability Assessment
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4% paraformaldehyde, Triton X-10, and 10% EDTA solution were provided by Biosharp (Anhui, China). The Hematoxylin–Eosin (HE) Staining Kit and DAPI were purchased from Beyotime (Chongqing, China). A Calcein/PI Live/Dead Cell Viability Kit was supplied by Sigma Chemical Co. (St. Louis, MO, United States). Rhodamine phalloidin, Alexa Fluor 488, and anti-paxillin antibody were supplied by Abcam (Shanghai). Sprague–Dawley (SD) rats aged 12 weeks (400 ± 40 g) were provided by Hunan SJA Laboratory Animal Co.. LTD. (Hunan, China).
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