Mtt powder

A549 and A549/DDP cell lines were purchased from the ATCC Cell Bank (USA). DMEM medium, fetal bovine serum (FBS), EDTA, and penicillin-streptomycin were bought from Hyclone (USA). Dimethyl sulfoxide and MTT powder were from Gibco. Tyresin-EDTA was from Sigma (USA). PVDF membrane was from Pall Life Sciences. Western blot-related chemical reagents were obtained from Beyotime. ECL reagent was from Amersham Biosciences. ERCC1, BRCA1, PI3K, AKT, phosphorylated PI3K, and phosphorylated AKT monoclonal antibodies, and HRP-tagged IgG secondary antibody were from Cell Signaling. Caspase 3 activity kits were purchased from Pall Life Sciences. RNA extraction kits and reverse transcription kits were from Axygen (USA). Other common reagents were purchased from Sangon. The Labsystem version 1.3.1 microplate reader was purchased from Bio-Rad (USA).

CML cell line K562 was purchased from the ATCC cell bank (USA). RPMI 1640 culture medium and penicillin/streptomycin were purchased from Hyclone (USA). As₄S₄ was extracted from red orpiment crystals and was dissolved in RPMI 1640 medium (160 mM stock). DMSO and MTT powder were purchased from Gibco (USA). Trypsin-EDTA buffer was obtained from Sigma (USA). PVDF membrane and caspase 3 activity kit were purchased from Pall Life Sciences (USA). Reagents for Western blotting were provided by Beyotime (China). ECL kits were purchased from Amersham Biosciences (USA). Rabbit anti-human Bcl2 monoclonal antibody and goat anti-rabbit IgG conjugated with horseradish peroxidase (HRP) were obtained from Cell Signaling (USA). Annexin V-FITC apoptotic assay kit, RNA extraction kit, reverse transcription kit, and microplate reader were obtained from BD (USA). MiR-181 mimic was purchased from Shanghai Genechem Co., Ltd. (Shanghai, China). DNA amplifier was purchased from PE (Gene Amp PCR System 2400). Other common reagents were purchased from Sangon (China).