Total RNA from leaves of Shuchazao was isolated using RNAiso-matefor Plant Tissue (Takara, Dalian, China) and RNAiso Plus (Takara, Dalian, China). The cDNA was synthesized by reverse transcription from total RNA using PrimeScriptRT Master Mix (Takara, Dalian, China).
Rnaiso mate for plant tissue
Manufactured by Takara Bio
Sourced in China
RNAiso-mate for Plant Tissue is a laboratory product designed for the extraction and isolation of high-quality RNA from plant tissues. It is a reagent system specifically formulated to facilitate the efficient isolation of total RNA from various plant sources.
Automatically generated - may contain errors
Lab products found in correlation
Rnaiso plus, by Takara Bio (9 mentions)
Primescript rt master mix, by Takara Bio (5 mentions)
Phusion high fidelity dna polymerase, by New England Biolabs (4 mentions)
Minibest agarose gel extraction kit, by Takara Bio (2 mentions)
Smarter race cdna amplification kit, by Takara Bio (1 mentions)
Cfx96 platform, by Bio-Rad (1 mentions)
Hieff qpcr sybr green master mix, by Yeasen (1 mentions)
Gel extraction kit, by CWBIO (1 mentions)
Hiscript 3 rt supermix for rt qpcr, by Vazyme (1 mentions)
Rnase free dnase, by Promega (1 mentions)
9 protocols using rnaiso mate for plant tissue
1
Leaf Total RNA Extraction and cDNA Synthesis
2
Isolation and Characterization of CsUGT75L12
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was isolated and extracted from different samples using RNAiso-mate for Plant Tissue (Takara, DaLian, China; Code: D325A) and RNAiso Plus (Takara, DaLian, China; Code: D9108B) according to the manufacturer’s instructions. cDNA was synthesized by reverse transcription from the total RNA using PrimeScript RT Master Mix (Takara, DaLian, China;Cat: RR036A). The 3′-cDNA and 5′-cDNA fragments of candidate genes were synthesized using a SMARTer™ RACE cDNA Amplification Kit (Clontech, USA; Cat. Nos. 634923 and 634924). A sequence for the full length open reading frame (ORF) was obtained by assembling the obtained 5′ and 3′ sequences. Subsequently, the ORF sequence was amplified using Phusion® High-Fidelity DNA Polymerase (New England Biolabs, USA). The amplified product was designated as ‘CsUGT75L12’, using the naming regulations set forth by the UGT Nomenclature Committee, and submitted to NCBI, (GenBank TM accession number KP682364). All of the primers sequences that were used in the study are listed in Suppl Table S1 .
Sequence alignments were conducted using Dnaman 7 software (Lynnon, Canada). The phylogenetic tree was generated by the neighbor-joining method within the MEGA 5.0 program using 1,000 bootstrap replications.
Sequence alignments were conducted using Dnaman 7 software (Lynnon, Canada). The phylogenetic tree was generated by the neighbor-joining method within the MEGA 5.0 program using 1,000 bootstrap replications.
3
Isolating RNA from Camellia sinensis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was isolated from leaves of Camellia sinensis var. sinensis cv. Shuchazao using RNAiso Mate for Plant Tissue (Takara, Dalian, China) and RNAiso Plus (Takara, Dalian, China) according to the manufacturer’s instructions. Next, cDNA was synthesized from total RNA by reverse transcription using PrimeScript RT Master Mix (Takara, Dalian, China).
4
Quantitative Gene Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA from six dissociated leaf tissues was isolated using RNAiso‐mate for Plant Tissue (Takara, Dalian, China) and RNAiso Plus (Takara) according to the manufacturer's instructions. The cDNA was synthesized by reverse transcription from total RNA using PrimeScriptRT Master Mix (Takara). Quantitative real‐time PCR (q‐RT‐PCR) assays were performed on a CFX96 platform (Bio‐Rad, CA), using the Hieff® qPCR SYBR Green Master Mix (YEASEN, Shanghai, China) according to the manufacturer's instructions and calculated by the comparative CT method (Livak and Schmittgen, 2001 (link)). Transcript abundances of CsUBI (CSS0007748, ubiquitin‐conjugating enzyme) and CsACTIN (CSS0008920, actin 7 isoform 1) were determined as references. Table S15 gives all primer sequences.
5
Isolation and Cloning of Plant Transcripts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was isolated from leaves of the Shuchazao cultivar using RNAiso‐mate for Plant Tissue (Takara, Dalian, China) and RNAiso Plus (Takara) according to the manufacturer’s instructions. cDNA was synthesized from total RNA by reverse transcription using HiScript III RT SuperMix for RT-qPCR (Vazyme Biotech Co. Ltd, Nanjing, China). Open-reading frame sequences were amplified using Phusion High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA). The PCR products were purified with a Gel Extraction Kit (CWBIO, Jiangsu, China), ligated into the pGEX-4T1 vector, and subsequently transformed into Trans1T1 competent cells.
6
Maize and Tobacco Plant Growth Conditions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tobacco plants (Nicotiana tabacum cv. xanthi) were grown on MS medium (Murashige and Skoog, 1962 (link)) with 2% (w/v) sucrose and 0.8% (w/v) agar with cool white fluorescent light (~200 μmol/m2/s) under a 12 h light/12 h dark photoperiod at 21–25°C/15–18°C (day/night). Plants in soil were grown in a greenhouse under a 14 h photoperiod, comprised of natural daylight supplemented with lamps (120–150 µEm2/s) at 21–25°C/15–18°C (day/night). The sequenced maize (Zea mays L.) cultivar B73 was grown in greenhouse as described previously (Li et al., 2013 (link)).
For expression pattern analyses of ZmmiR156 in maize plants, juvenile (1-month-old) and old (4-month-old) maize plants or roots of 2-week-old seedlings treated with 15% PEG, 100 mM NaCl or 50 μM ABA were used. Total RNA was extracted from different tissues with RNAiso Plus and RNAiso-mate for Plant Tissue (TaKaRa, Dalian, China), and treated with RNase-free DNase (Promega, Shanghai, China).
For expression pattern analyses of ZmmiR156 in maize plants, juvenile (1-month-old) and old (4-month-old) maize plants or roots of 2-week-old seedlings treated with 15% PEG, 100 mM NaCl or 50 μM ABA were used. Total RNA was extracted from different tissues with RNAiso Plus and RNAiso-mate for Plant Tissue (TaKaRa, Dalian, China), and treated with RNase-free DNase (Promega, Shanghai, China).
7
Camellia sinensis RNA Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For analysis of gene expression, the leaves of Camellia sinensis var. sinensis. “Shuchazao” were collected for RNA extraction. The total RNA was isolated by RNAiso-mate for plant tissue (Takara, Dalian, China) and RNAiso Plus (Takara, Dalian, China). The cDNAs were amplified using Phusion® High-Fidelity DNA Polymerase (New England Biolabs, MA, USA), and the PCR products were purified via a MiniBEST agarose gel extraction kit (Takara, Dalian, China). The amplified PCR product was ligated into the pGEX-4T1 vector and transformed into TransT1-competent cells for sequencing.
8
Isolation and Cloning of Tea Plant Transcripts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
According to the manufacturer's instructions, total RNA was isolated using RNAisomate for Plant Tissue (Takara, Dalian, China) and RNAiso Plus (Takara, Dalian, China). The cDNA was synthesized by reverse transcription from the total RNA using PrimeScript RTMaster Mix (Takara, DaLian, China). The open reading frame sequence was amplified using Phusion High‐Fidelity DNA Polymerase (NewEngland Biolabs, MA, USA), and the PCR products were purified with a MiniBEST Agarose Gel Extraction Kit (Takara, Dalian, China) and ligated into the PGEX‐4T1 simple vector and subsequently transformed into TransT1 competent cells. Wild type tea plant tissues of different developmental stages (first leaf, second leaf, and third leaf), were harvested, and nucleic acid was isolated by the same method.
9
Extraction of Total RNA from Ipomoea asprella Root
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Two-year-old, potted I. asprella was collected from the Planting Base in Meizhou, Guangdong province, China. The I. asprella root was flushed under running tap water to remove soil and other attachments. After quick drying with bibulous papers, the root tissue was cut into approximately 1-mm-thick segments, snap frozen in liquid nitrogen and stored at −80 °C until further processing. Total RNA of the root was isolated using RNAiso Plus and RNAiso-mate for Plant Tissue (Takara, Dalian, China) following the product manual. The integrity, purity and concentration of the total RNA were analysed using agarose gel electrophoresis and ultraviolet spectroscopy.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!