Iraffinity 1s fourier transform ir ftir spectrometer

NMR spectra were acquired on a Bruker Avance III NMR spectrometer (Bruker AMX-400 NMR spectrometer) with the magnetic field of 11.74 Tesla. HR-ESI-MS were acquired on a Bruker Q-TOF mass spectrometer. The IR spectra of the compounds were recorded using an IRAffinity-1S Fourier transform IR (FTIR) spectrometer (Shimadzu, Tokyo, Japan). Chemical purities of the compounds were checked by classical TLC applications performed on silica gel 60 F254 (Merck KGaA, Darmstadt, Germany). The deuterated chloroform (CDCl₃, deuterated ratio, 99.8%) with TMS as the internal referent were produced by Cambridge Isotope Labo-ratories, Inc. (Andover, MA, USA). All other chemicals were obtained either from Sigma-Aldrich (Sigma-Aldrich Corp., St. Louis, MO, USA) or Merck (Merck KGaA, Darmstadt, Germany) and used without further purification.

All chemicals in this study were obtained either from Sigma-Aldrich (Sigma-Aldrich Corp., St. Louis, MO, USA) or Merck (Merck KGaA, Darmstadt, Germany), and used without further chemical purifications. Melting points of the compounds were measured by using an automatic melting point determination instrument (MP90, Mettler-Toledo, Columbus, OH, USA) and were presented as uncorrected. ¹H- and ¹³C-NMR spectra were recorded in DMSO-d₆ by a Bruker digital FT-NMR spectrometer (Bruker Bioscience, Billerica, MA, USA) at 300 MHz and 75 MHz, respectively. The IR spectra of the compounds were recorded using an IRAffinity-1S Fourier transform IR (FTIR) spectrometer (Shimadzu, Tokyo, Japan). HRMS studies were performed on an LCMS-IT-TOF system (Shimadzu, Tokyo, Japan). Chemical purities of the compounds were checked by classical TLC applications performed on silica gel 60 F₂₅₄ (Merck KGaA, Darmstadt, Germany).

All chemicals were obtained either from Sigma-Aldrich Corp. (St. Louis, MO, USA) or Merck KGaA (Darmstadt, Germany), and used without further chemical purification. Melting points of the compounds were measured by using an automatic melting point determination instrument (MP90, Mettler-Toledo, Columbus, OH, USA) and were presented as uncorrected. ¹H- and ¹³C-NMR spectra were recorded in DMSO-d₆ by a Bruker digital FT-NMR spectrometer (Bruker Bioscience, Billerica, MA, USA) at 300 MHz and 75 MHz, respectively. The IR spectra of the compounds were recorded using an IRAffinity-1S Fourier transform IR (FTIR) spectrometer (Shimadzu, Tokyo, Japan). MS studies were performed on an LCMS-8040 tandem mass system (Shimadzu, Tokyo, Japan). Chemical purities of the compounds were checked by classical TLC applications performed on silica gel 60 F254 (Merck KGaA).

All chemicals used in the synthesis studies were obtained from Merck Chemicals (Merck KGaA, Darmstadt, Germany) or Sigma-Aldrich Chemicals (Sigma-Aldrich Corp., St. Louis, Mo., USA). The MP90 digital melting point apparatus (Mettler Toledo, OH, USA) was used to determine the melting points (MPs) of the resulting compounds, which were presented uncorrected. Bruker 300-MHz and 75-MHz digital Fourier transform (FT) NMR spectrometers (Bruker Bioscience, Billerica, MA, USA) in dimethyl sulfoxide (DMSO-d₆) were used to record the ¹H NMR and ¹³C NMR spectra, respectively. In the NMR spectra, splitting patterns were determined as follows: s, singlet; d, doublet; t, triplet; dd, double doublet; m, multiplet. Coupling constants (J) are reported in units of Hertz (Hz). An IRAffinity-1S Fourier transform IR (FTIR) spectrometer (Shimadzu, Tokyo, Japan) was used to record the IR spectra of the compounds. Mass spectra were recorded on an LCMS-8040 tandem mass spectrometer (Shimadzu, Kyoto, Japan) by means of electrospray ionization (ESI). Silica gel 60 F254 thin-layer chromatography (TLC; Merck KGaA, Darmstadt, Germany) was used to control the purity of the obtained compounds.

All chemicals were obtained either from Sigma-Aldrich (Sigma-Aldrich Corp., St. Louis, MO, USA) or Merck (Merck KGaA, Darmstadt, Germany), and used without further chemical purification. Melting points of the compounds were measured by using an automatic melting point determination instrument (MP90, Mettler-Toledo, OH, USA) and were presented as uncorrected. ¹H and ¹³C-NMR spectra were recorded in DMSO-d₆ by a Bruker digital FT-NMR spectrometer (Bruker Bioscience, MA, USA) at 300 MHz and 75 MHz, respectively. The IR spectra of the compounds were recorded using an IRAffinity-1S Fourier transform IR (FTIR) spectrometer (Shimadzu, Tokyo, Japan). The HRMS studies were performed on an LCMS-IT-TOF system (Shimadzu, Tokyo, Japan). Chemical purities of the compounds were checked by classical TLC applications, which was performed on a silica gel 60 F254 (Merck KGaA, Darmstadt, Germany).