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51 protocols using hexadecylamine

1

Synthesis of Iron Nanoparticles under Inert Atmosphere

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Synthesis of iron NPs (FeNP) were performed under argon atmosphere by using Fisher–Porter tubes, a glovebox and argon/vacuum lines. Mesitylene (+99%, Acros), toluene (99%, VWR Prolabo), methanol (96% STREM), acetic acid (98% Aldrich), and ethanol (99.9% Aldrich) were dried according to classical procedures. These solvents were distilled and degassed prior use. Iron bistrimethylsilylamide dimer, [Fe(N(SiMe3)2)2]2, was purchased from Nanomeps, hexadecylamine (HDA, Aldrich, 98%), oleic acid (OA, 99%, Alfa Aesar), cyclohexane (Aldrich, 99%), igepal CO-520 (Aldrich, 90%), tetraorthosilicate (TEOS, Aldrich, 98%), ammonia solution (28.0–30%, Sigma–Aldrich), poly(ethyleneglycol)monomethyl ether (PEG-OH) (5 kDa, Aldrich), and 3-(triethoxysilyl)propylisocyanate (TESPIC, Alfa Aesar) were used as received. Hexadecylammonium chloride (HDA.HCl) was prepared according to reference [27 (link)] from hexadecylamine (HDA, Aldrich, 90%). MilliQ water (18 MΩ) was used for all aqueous preparations.
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2

Colloidal Synthesis of Inorganic Nanomaterials

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Cadmium oxide (99.5%, powder), 2-ethylhexanoic acid (2-EHA, 99%), 1-octadecene (ODE, technical grade, 90%), oleylamine (OLA, technical grade, 70%), hexadecylamine (technical grade, 90%), trioctylamine (98%), trioctylphisphine (TOP, technical grade, 97%), selenium powder (powder, 100 mesh, 99.5%), zinc oxide (puriss, 99–100%), thiourea (TU, ACS Reagent,>99%), triethylene glycol dimethyl ether (TEGDME, 99%), hexadecylamine (technical grade, 90%), palmitic acid (BioXtra, ≥99%) zinc acetate dihydrate (ACS reagent, ≥99.0%), magnesium acetate tetrahydrate (ACS reagent, ≥98%), aluminium chloride hexahydrate (99%), gallium nitrate hydrate (99.9%), lithium chloride (anhydrous, ACS reagent, ≥99%), tetramethylammonium chloride (TMACl, reagent grade, ≥98%), potassium hydroxide (reagent grade, 90%), dimethyl sulfoxide (DMSO, anhydrous, ≥99.9%), and monoethanolamine (MEA, ACS reagent, ≥99.0%) were purchased from Sigma-Aldrich. n-Hexadecylphosphonic acid (97%) was from PlasmaChem GmbH. Methyl acetate (extra pure, 99%) was from Acros. Hexane, n-octane, toluene, acetone, methanol, ethanol, n-butanol, and isopropyl alcohol of spectroscopy grade were purchased from the local supplier Ekos-1. All reagents were used as received, without purification.
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3

Synthesis of Nanomaterials for Bioassays

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Phosphate saline buffer (PBS, pH 7.4), methanol, potassium hydroxide, polyoxyethyelene (20), sorbitan monolaurate (Tween 20), sulfuric acid, sodium citrate, tri-sodium citrate, hydrogen peroxide, chloroform and acetone were purchased from FUJIFILM Wako Pure Chemical Ind. Ltd. (Osaka, Japan). Tetramethylbenzidine (TMBZ) was purchased from Dojindo (Kumamoto, Japan). HAuCl4, N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC), bovine serum albumin (BSA), N-hydroxysuccinimide (NHS), 1-octadecene, tellurium (Te), l-cysteine, hexadecylamine (HDA), cadmium oxide (CdO), trioctylphosphine oxide (TOPO), trioctylphosphine (TOP), selenium (Se) and sulfur (S) were purchased from Sigma Aldrich (Saint Louis, MO, USA). Oleic acid was purchased from Nacalai Tesque (Kyoto, Japan).
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4

Fluorescent Probe Synthesis and Characterization

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Chlorotrimethylsilane, tetramethylammonium hydroxide pentahydrate (TMAH), cadmium oxide (CdO), octadecene (ODE), zinc oxide (ZnO), trioctylphosphine oxide (TOPO), selenium (Se), (3-aminopropyl)trimethoxysilane (3-APTMS), trioctylphosphine (TOP), hexadecylamine (HDA), sulfur, succinic anhydride, rhodamine 6G, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), HAuCl4·3H2O, tannic acid, TGA, MPA, l-cysteine, and GSH were purchased from Sigma Aldrich Co. LLC. (Saint Louis, MO, USA). Oleic acid was purchased from Nacalai Tesque Inc. (Kyoto, Japan). Methanol, tri-sodium citrate and potassium hydroxide (KOH), methanol, acetone, and chloroform were purchased from Wako Pure Chemical Ind. Ltd. (Osaka, Japan). An ultrapure Milli-Q Water System was used for sample preparation. The MB and synthetic DNA targets were purchased from FASMAC Co. Ltd. (Atsugi, Kanagawa, Japan). Black hole quencher-2 (BHQ-2) was used as the fluorescence quencher.
MB sequence: 5′-/NH2/GCGACTTTCAGTTATTATGCCGTTGTATTTGTCGC/BHQ-2/-3′
Full complementary DNA: AAATACAACGGCATAATAACTGAAA
Single nucleotide DNA: AAATACAACGTCATAATAACTGAAA
Noncomplementary DNA: TGAAGCTAACCGGTAAGCGCTATAG
The bold sequence is the stem sequence of the MB.
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5

Synthesis and Characterization of Lipid Structures

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Hexadecylamine, 5-chloro-2-nitrobenzotrifluoride, and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were purchased from Sigma-Aldrich, and silica gel (100–200 mesh) was purchased from S.D Fine Chemicals Private Limited. Other chemicals were purchased from Loba Chemie or Merck Limited unless mentioned otherwise. Solvents used were analytically pure and used without further purification.
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6

Formulation and Characterization of Hyaluronate Nanocarriers

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Low molecular weight Na hyaluronate was used (Bioiberica, Barcelona, Spain), which was measured by capillary viscosimetry as about 50 kDa. Dodecyl amine (DDA), hexadecyl amine (HDA), and cholesterol (CHOL) were from Sigma-Aldrich (Milan, Italy). Pyrene was from Fluka (Milan, Italy). Clotrimazole (CLO) was obtained from Sifavitor (Casaletto Lodigiano, Lodi, Italy). Ketoconazole was used as an internal standard and obtained from Erregierre (San Paolo D’Argon, Bergamo, Italy). All the other reagents were from Carlo Erba (Milan, Italy).
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7

Synthesis of Colloidal Nanocrystals

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Cadmium oxide (CdO, ≥99.99%), selenium (≥99.99%), trioctylphosphine oxide (TOPO, 90%), trioctylphosphine (TOP, 97%), hexadecylamine (HDA, 90%), oleic acid (OLA, 90%), 1-octadecene (ODE, 90%), 1-octanethiol (≥98%) and 3-mercaptopropionic acid (MPA, ≥99%) were purchased from Sigma-Aldrich. PEG5000k-methoxy (PEG-OMe, PLS-604), and PEG5000k-hydroxyl (PEG-OH, PBL-8083) were purchased from Creative PEGWorks. Hexanes (≥98.5%), toluene (≥99.5%), acetone (≥99.5%), methanol (≥99.8%) and citric acid (≥99.5%) were purchased from Fischer Scientific. All reagents were used as received, without further purification.
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8

Synthesis of Colloidal Nanocrystals

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Cadmium oxide (CdO, ≥99.99%), selenium (≥99.99%), trioctylphosphine oxide (TOPO, 90%), trioctylphosphine (TOP, 97%), hexadecylamine (HDA, 90%), oleic acid (OLA, 90%), 1-octadecene (ODE, 90%), 1-octanethiol (≥98%) and 3-mercaptopropionic acid (MPA, ≥99%) were purchased from Sigma-Aldrich. PEG5000k-methoxy (PEG-OMe, PLS-604), and PEG5000k-hydroxyl (PEG-OH, PBL-8083) were purchased from Creative PEGWorks. Hexanes (≥98.5%), toluene (≥99.5%), acetone (≥99.5%), methanol (≥99.8%) and citric acid (≥99.5%) were purchased from Fischer Scientific. All reagents were used as received, without further purification.
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9

Synthesis of Monodisperse Iron Oxide Nanoparticles

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All reactants used were commercially available: hexadecylamine (HDA) 98% purity, oleic acid (OA) 99% purity, iron pentacarbonyl, dibenzyl ether 98% purity, ethyl acetate (99.5% purity), tetramethylammonium hydroxide (TMAH) solution in water (25% wt), and benzyl ether (98% purity) were purchased from Sigma-Aldrich Saint Louis, MO, USA and were used as received. Absolute ethanol and n-hexane 99% reagent grade were supplied by Scharlau, Cham Germany.
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10

Synthesis of Semiconductor Nanocrystals

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Cadmium oxide (CdO, 99.5%), trioctylphosphine
oxide (99%), trioctylphosphine (TOP, 97%), selenium powder (Se, 99.99%),
and lead(II) acetate trihydrate (Pb(CH3CO2)2·3H2O, 99.999%) were purchased from Strem
Chemicals. Octadecylphosphonic acid (99%) was purchased from Polycarbon
Industries. Copper(II) acetylacetonate (Cu(acac)2, 97%),
hexadecylamine (98%), tetrakis(acetonitrile)copper(I) hexafluorophosphate
(99.99%), octadecene (technical grade, 90%), 1-dodecanethiol (≥98%),
oleylamine (70%), tetrachloroethylene (≥99%), anhydrous chloroform,
methanol, toluene, and tetrahydrofuran were purchased from Sigma-Aldrich.
All chemicals were used without any further purification.
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