HCC cell lines, HepG2, Huh7, PLC/PRF-5 and Hep3B, were purchased from the Korean Cell Line Bank (Seoul National University, Korea). Cells were cultured in Dulbecco’s modified Eagle medium (DMEM) with 10% fetal bovine serum (FBS), 5% penicillin–streptomycin, and 5% sodium pyruvate, and grown in an atmosphere containing 5% CO2 at 37 °C. Cells were incubated with or without quercetin (Sigma-Aldrich, MO) as indicated in each experiment. Resveratrol was also purchased from Sigma-Aldrich. Cell images were captured by microscopy (Leica, Germany). For functional experiments, HepG2 cells were transfected with siRNA against the p53 gene (sip53, forward; 5′-GACUCCAGUGGUAAUCUACTT-3′ and reverse; 5′-GUAGAUUACCACUGGAGUCTT-3′) using Lipofactor-2000 (Aptabio, Korea).
Hep3b
Manufactured by Korean Cell Line Bank
Sourced in United States, Cameroon
Hep3B is a human cell line derived from a liver cancer patient. It is commonly used in research related to hepatocellular carcinoma. The Hep3B cell line exhibits epithelial-like morphology and is adherent in culture.
Automatically generated - may contain errors
Lab products found in correlation
Hepg2, by Korean Cell Line Bank (25 mentions)
Plc prf 5, by Korean Cell Line Bank (13 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (12 mentions)
Snu449, by Korean Cell Line Bank (9 mentions)
Sk hep 1, by Korean Cell Line Bank (9 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (8 mentions)
Penicillin, by Thermo Fisher Scientific (6 mentions)
Streptomycin, by Thermo Fisher Scientific (6 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (6 mentions)
Hep3b, by Thermo Fisher Scientific (6 mentions)
Snu398, by Korean Cell Line Bank (5 mentions)
Snu475, by Korean Cell Line Bank (5 mentions)
Mcf 7, by Korean Cell Line Bank (4 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (4 mentions)
Snu 387, by Korean Cell Line Bank (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (3 mentions)
G418 sulfate, by Merck Group (3 mentions)
Turbofect transfection reagent, by Thermo Fisher Scientific (3 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (3 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (3 mentions)
53 protocols using hep3b
1
HCC Cell Lines Cultured with Quercetin
2
Sourcing of HCC Cell Lines
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3
Culturing and Treating Human Liver Cancer Cells
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The human HCC lines (Hep3B, HepG2, Huh7, PLC/PRF5, SNU387, SNU398, SNU449, SNU475, and SNU761) were purchased from the Korean Cell Line Bank. Huh-BAT cells were obtained from the Liver Research Institute, Seoul National University. Gefitinib was kindly provided by AstraZeneca Korea. Sorafenib and silibinin were purchased from Selleck Chemicals (Houston, TX, USA) and Sigma-Aldrich Co. (St. Louis, MO, USA), respectively.
4
Culturing and Maintaining Cell Lines
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Hep3B, HepG2, and Huh7 cells were purchased from the Korean Cell Line Bank (Seoul, Korea) and cultured in Dulbecco’s minimum essential medium (Corning, NY, USA), supplemented with 10% fetal bovine serum (FBS; Corning) and 1% penicillin–streptomycin (P/S; Corning). Human NK-92 cells were purchased from the American Type Culture Collection (Manassas, VA, USA) and cultured in α-minimum essential medium (Corning), which was composed of 12.5% FBS (Corning), 12.5% horse serum (HS; Sigma-Aldrich, St. Louis, MO, USA), 1% P/S (Corning), 0.2 mM Myo-inositol (Sigma-Aldrich), 0.1 mM 2-mercaptoethanol (Sigma-Aldrich), 0.02 mM folic acid (Sigma-Aldrich), 10 ng/mL Interleukin (IL)-2 (Miltenyi Biotec, Bergisch Gladbach, Germany), and 20 ng/mL IL-15 (Miltenyi Biotec) activating cytokines. Normal human liver THLE-2 cells were obtained from Dr. KP Kim (Asan Medical Center, Seoul, Korea) and were cultured in bronchial epithelial cell growth medium (BEGM Bullet Kit; Lonza, Basel, Switzerland) as per the manufacturer’s protocol. All cells were cultured at 37 °C with 5% CO2.
5
Culturing Human Cell Lines
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Human adipose tissue-derived MSCs and human bone marrow-derived MSCs were purchased from Cyagen Biosciences (China). MSCs were incubated at 37°C inside a 5% CO2 humidified incubator using human mesenchymal stem cell growth medium (Cyagen Biosciences, China). A549, MDA-MB-231, MIA PaCa-2, A375, SK-BR-3, HPAC, HCT116, BJ, and HeLa cells were purchased from ATCC. SNU-398, DU145, Hep3B, and SK-HEP-1 were purchased from Korean Cell Line Bank and 293A cell (a subclone of HEK293) was purchased from Invitrogen (R70507). Cells were grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (HyClone Laboratories, Logan, UT, United States) at 37°C under 5% CO2.
6
Hepatocellular Carcinoma Cell Culture
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Hepatocellular carcinoma cell lines HepG2, Huh7, and Hep3B were obtained from the Korean cell line bank (Seoul, South Korea) and were cultured in DMEM (Invitrogen, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 U/ml streptomycin (Lonza, Basel, Switzerland) at 37°C and 5% CO2. Wnt signaling inhibitor CWP232228 is designed by JW Pharmaceutical Corporation (Seoul, Korea).
7
Evaluating Sorafenib's Effects on Liver Cells
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A total of 293 cells and human liver cells (Huh7, Hep3B and HepG2) were purchased from the Korean Cell Line Bank (KCLB, Seoul, Korea). LX-2 cells were kindly provided by Dr Park (Yonsei University, Seoul, Korea). L-O2 cells (an immortalized normal liver cell line) were provided by Dr Shin (Sung-Kyun-Kwan University, Gyeonggi, Korea). The cell lines were cultured in a growth medium consisting of Dulbecco's modified Eagle's medium (DMEM; HyClone; Cytiva) and MEM supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), and 1% antibiotics (Gibco; Thermo Fisher Scientific, Inc.) in a 5% CO2-humidified incubator. The cell groups were treated with various concentrations of sorafenib (0, 0.1, 0.5, 1, 5, and 10 µM) in 10% FBS-supplemented growth media for 4 days. Sorafenib concentration referred to Liang et al reports (16 (link)).
8
Cytotoxicity Evaluation of Cancer Cell Lines
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The cancer cell lines AGS, HeLa, HEP3B, and HT-29 were obtained from the Korea Cell Line Bank, Seoul, Korea. The AGS and HT-29 cells were cultured in Roswell Park MEMorial Institute (RPMI)-1640 (Hyclone Laboratories, Utah, USA) supplemented with 10% fetal bovine serum. HeLa cells were cultured in MEM (Hyclone Laboratories, Inc., Utah, USA) supplemented with 10% fetal bovine serum, while HEP3B cells were cultured in DMEM (Hyclone Laboratories, Utah, USA) supplemented with 10% fetal bovine serum in a 5% CO2 atmosphere at 37℃. Cell viability was analyzed using an MTT assay kit (Roche Molecular Biochemicals, Mannheim, Germany) according to the manufacturer's instruction. Briefly, the treated cells were incubated with the MTT reagent for 4h and lysed with a lysis buffer. The absorbance was measured from 550 nm to 650 nm after an overnight incubation. The 50% inhibitory concentrations (IC50s) were calculated from four concentrations of the extracts (100, 250, 500, 750 µg/mL).
9
Transfection of Hepatocellular Carcinoma Cells
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The hepatocellular carcinoma cell lines HepG2 and Hep3B cells were purchased
from the Korean Cell Line Bank (KCLB, Seoul, Korea). These cells were then
cultured in Dulbecco's modified Eagle's medium (WelGENE, Seoul,
Korea) supplemented with 10% fetal bovine serum,
100 U ml−1 penicillin and
100 μg ml−1 streptomycin (Gibco, Grand
Island, NY, USA) in a humidified 5% CO2/95% air
atmosphere at 37 °C.
For transfection, cells were plated in a 6-well plate (2 ×
105 cells per well) or 100 mm dish (2 ×
106 cells per dish) and then transfected with the appropriate
plasmid(s) in combination with Lipofectamine 2000 reagent (Invitrogen,
Carlsbad, CA, USA). pSilencer 3.1-H1 puro ANT2 or scrambled small
interfering RNA vectors were transfected into the cells. Transfected cells
were then cultured for 4 h, and the culture media were replaced with
fresh media supplemented with 10% fetal bovine serum. The cells were
harvested 24–48 h after transfection. Negative mimics, miR-19a
and miR-96 mimics, negative inhibitors and miR-19a and miR-96 inhibitors
were transfected into the cells using the same method.
from the Korean Cell Line Bank (KCLB, Seoul, Korea). These cells were then
cultured in Dulbecco's modified Eagle's medium (WelGENE, Seoul,
Korea) supplemented with 10% fetal bovine serum,
100 U ml−1 penicillin and
100 μg ml−1 streptomycin (Gibco, Grand
Island, NY, USA) in a humidified 5% CO2/95% air
atmosphere at 37 °C.
For transfection, cells were plated in a 6-well plate (2 ×
105 cells per well) or 100 mm dish (2 ×
106 cells per dish) and then transfected with the appropriate
plasmid(s) in combination with Lipofectamine 2000 reagent (Invitrogen,
Carlsbad, CA, USA). pSilencer 3.1-H1 puro ANT2 or scrambled small
interfering RNA vectors were transfected into the cells. Transfected cells
were then cultured for 4 h, and the culture media were replaced with
fresh media supplemented with 10% fetal bovine serum. The cells were
harvested 24–48 h after transfection. Negative mimics, miR-19a
and miR-96 mimics, negative inhibitors and miR-19a and miR-96 inhibitors
were transfected into the cells using the same method.
10
Profiling Biotin Receptor Expression
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Twelve biotin receptor-positive cell lines; human lung carcinoma cells (A549), human cervical cancer cells (HeLa), human breast cancer cells (MCF7, MDA-M231), human liver cancer cells (HepG2, Huh7, Hep3B), human prostate cancer cells (Du145, PC3), human gastric cancer cells (NCI-N87, AGS), human pancreatic cancer cell (Panc-1), and a biotin receptor-negative cell: human normal embryonal kidney epithelial cell (293T) were purchased from the Korean Cell Line Bank (Seoul, Republic of Korea). Two more biotin receptor-negative normal cell lines, human normal fibroblast cells obtained from fetal lung (WI-38 cells) or neonatal foreskin (BJ) cells were purchased from the Korean Cell Line Bank (Seoul, Republic of Korea) or Modern Cell & Tissue Technologies (MCTT, Seoul, Republic of Korea). The cells were cultured in either Roswell Park Memorial Institute medium (RPMI-1640, GIBCO BRL, Grand Island, NY, USA) or Dulbecco’s Modified Eagle’s Medium (DMEM, GIBCO BRL) supplemented with 10% fetal bovine serum (FBS, GIBCO), and 1% penicillin and streptomycin (GIBCO), at 37 °C in a humidified atmosphere containing 5% of CO2. When the cell density reached 70–80% of confluence, subculturing was considered complete. The medium was changed approximately every 3 to 4 days.
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