Pez m02 vector

Manufactured by GeneCopoeia

Sourced in United States

The PEZ-M02 vector is a plasmid designed for the expression of recombinant proteins in mammalian cell lines. The vector features a cytomegalovirus (CMV) promoter for strong, constitutive expression of the gene of interest, as well as a multiple cloning site for convenient insertion of the target DNA sequence. The PEZ-M02 vector also includes a selectable marker for antibiotic resistance, allowing for the selection of transfected cells.

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Lab products found in correlation

Sds page kit, by Beyotime (1 mentions) Dapi staining kit, by Beyotime (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Ripa buffer, by Beyotime (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Ecl kit, by Beyotime (1 mentions) Bca protein quantitative kit, by Beyotime (1 mentions)

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PEZ-M02 (3 citations)

2 protocols using pez m02 vector

Bag-1 Protein Interactome Analysis

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Bag-1 expression plasmid was generated by Capital Biosciences (MD, USA). C-terminal TAP (tandem affinity purification) tag was added to human Bag-1 cDNA ORF after removing the stop codon, and the construct was cloned into pEZ-M02 vector (GeneCopoeia, MD, USA).
All antibodies were purchased from Cell Signaling Technology. The monoclonal primary antibodies were mouse α-Bag-1, rabbit α-β-actin, rabbit α-Bcl-2, rabbit α-Bax, rabbit α-Bad, rabbit α-Phospho-Bad (Ser136), rabbit α-Phospho-Bad (Ser112), rabbit α-C-Raf, rabbit α-Phospho-C-Raf (Ser338), rabbit α-B-Raf, rabbit α-Phospho-B-Raf (Ser445), rabbit α-A-Raf, rabbit α-14-3-3, rabbit α- Akt, rabbit α-Phospho-Akt (Ser473), rabbit α-Hsp70 and rabbit α-Vinculin.

Kizilboga T., Baskale E.A., Yildiz J., Akcay I.M., Zemheri E., Can N.D., Ozden C., Demir S., Ezberci F, & Dinler-Doganay G. (2019). Bag-1 stimulates Bad phosphorylation through activation of Akt and Raf kinases to mediate cell survival in breast cancer. BMC Cancer, 19, 1254.

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Investigating IL-37 Mediated Modulation of Liver Cancer

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Human liver cancer cell lines, HepG2 and MHCC97H were purchased from the Cell Bank of Typical Culture Preservation Committee of Chinese Academy of Sciences (Shanghai, China), where the cell lines were authenticated via STR profiling. IL-37 gene (NM_014439) was cloned into a pEZ-M02 vector (Genecopoeia, Inc.) by the manufacturer. Lipofectamine 3000 was purchased from Invitrogen; Thermo Fisher Scientific, Inc. Rabbit anti-human IL-37 (cat. no. ab153889), NF-κB (cat. no. D14E12) and GAPDH primary antibody (cat. no. AB-P-R 001) were obtained from Abcam, Cell Signaling Technology, Inc., and Goodhere Technology, respectively. HRP-labeled goat anti-mouse IgG (cat. no. A0216), HRP-labeled Goat Anti-Rabbit IgG (cat. no. A0208), ECL kit, BCA Protein Quantitative kit, RIPA buffer, SDS-PAGE kit and DAPI staining kit were obtained from Beyotime Biotechnology. PVDF membranes were purchased from EMD Millipore.

Li P., Guo H., Wu K., Su L., Huang K., Lai R., Deng Z., Li S., Ouyang P., Wang Y., Chen Z., Zhou G, & Wang S. (2020). Decreased IL-37 expression in hepatocellular carcinoma tissues and liver cancer cell lines. Oncology Letters, 19(4), 2639-2648.

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