5975 c insert 143 xl ei ci msd detector

Five milliliters of medium (the mixture of dodecane and medium) was sampled and centrifuged at 12,000g for 10 min, and then 50 μL of the dodecane layer was transferred to another tube at − 20 °C for further analysis. α-Terpineol was identified by GC–MS (Agilent Technologies 7890 A GC system equipped with a 5975 C insert 143 XL EI/CI MSD Detector) with a DB-WAX column (30 m × 0.32 mm × 0.25 μm). The amount of α-Terpineol was determined using linear calibration curves. For GC analysis, 1 μL of dodecane sample was injected with a split ratio of 20:1 and nitrogen was used as the carrier gas with a flow rate of 1 mL/min. The injector and detector temperature were maintained at 250 °C. The oven temperature was as follows: 80°C for 1 min and sequentially increased at the rate of 10 °C/min to 180 °C and 30 °C/min to 250 °C.

LP was identified and quantified by GC or GC–MS. Dodecane extracts (1 μL) separated from the yeast bi-phase culture, were filtrated and analyzed by GC–MS using an Agilent Technologies 7890A GC system equipped with a 5975C insert 143 XL EI/CI MSD Detector with an HP-5 ms chromatographic column. Helium was used as the carrier gas at a flow rate of 1 mL/min. The oven temperature was kept at 150 °C for 5 min, increased to 250 °C at the rate of 5 °C/min, and finally held at 250 °C for 5 min. The injector and detector temperatures were 250 and 260 °C, respectively. LP amounts were determined using the internal standard 1-eicosene [33 (link)–35 (link)].
Ethyl acetate extracts were analyzed by LC–MS or LC in order to identify and quantify LA. LC–MS was performed on a Thermo Fisher LCQ Advantage MAX instrument equipped with an electrospray ionization detector with a C18 column (4.6 mm × 250 mm). Samples were injected at a column flow of 1 mL/min in the mobile phase (methanol: water: formic acid = 87:13:0.02). LA amounts were determined using standard curves.

A measure of 2 mL of the fermented liquor was taken, and the same amount of n-hexane was added. The organic solvent and medium mixture were incubated at 200 rpm and 25°C for 1 h before being centrifuged at 4,000 g for 5 min. Following the extraction process, a measure of 500 μL from the n-hexane layer was carefully transferred into a vial made of darkened glass in order to facilitate subsequent analysis. α-Terpineol was identified by GC-MS (Agilent Technologies 7890A GC system equipped with a 5975C insert 143 XL EI/CI MSD detector) with a DB-WAX column (30 m × 0.32 mm × 0.25 μm). The amount of α-terpineol was determined from linear calibration curves (Supplementary Figure S1). The standard α-terpineol was purchased from Solarbio (China, GC ≥ 99.0%). For GC analysis, 1 μL of the n-hexane sample was injected with a split ratio of 10:1, and nitrogen was used as the carrier gas with a flow rate of 1 mL/min. The injector temperature and detector temperature were maintained at 250°C and 260°C, respectively. The oven temperature was maintained as follows: 80°C for 1 min and sequentially increased at the rate of 10°C/min to 180°C and 10°C/min to 250°C.