Optical rotations were determined using a Jasco P-1020 polarimeter. Infrared (IR) spectra were obtained using a Jasco FT/IR-4600 Fourier-transform infrared spectrometer. Proton and carbon nuclear magnetic resonance (1H and 13C NMR) spectra were recorded with chloroform (in CDCl3) on the following instrument: a JEOL JNM-AL500 (1H at 500 MHz and 13C at 125 MHz, JEOL, Tokyo, Japan). Mass spectra were determined by a Bruker Daltonics micrOTOF focus (ESI-TOF) (Bruker, Billerica, MA, USA) mass spectrometer. Thin layer chromatography was performed on a Wakogel B5F. HPLC was performed with a Hitachi LaChrom Elite system composed of an Organizer, L-2400 UV Detector, and L-2130 Pump. All reactions were carried out under an argon atmosphere in dried glassware unless otherwise noted. CH2Cl2 was distilled from diphosphorus pentoxide and then calcium hydride and dried over MS4A; toluene was distilled from diphosphorus pentoxide and dried over MS4A; and THF and diethyl ether were distilled from sodium/benzophenone immediately prior to use. All reagents were purchased from Tokyo Kasei Kogyo Co., Ltd.; Kanto Chemical Co., Inc.; or Aldrich Chemical Co., Inc.; and were used without further purification unless otherwise noted. MNBA was purchased from Tokyo Kasei Kogyo Co., Ltd. (TCI M1439).
L 2130 pump
Manufactured by Hitachi
Sourced in Japan, United States
The Hitachi L-2130 pump is a precision liquid handling device designed for laboratory applications. It is capable of delivering a wide range of flow rates with high accuracy and repeatability. The pump's core function is to provide controlled and reliable fluid movement for various experimental and analytical processes.
Automatically generated - may contain errors
Lab products found in correlation
L 2400 uv detector, by Hitachi (14 mentions)
L 2200 autosampler, by Hitachi (11 mentions)
Hplc system, by Hitachi (6 mentions)
P 1020 polarimeter, by Jasco (5 mentions)
L 2200, by Hitachi (5 mentions)
Lachrom elite system, by Hitachi (5 mentions)
Uv vis l 2420 detector, by Hitachi (5 mentions)
L 2455, by Hitachi (4 mentions)
Lachrom elite, by Hitachi (4 mentions)
Ft ir 4600 fourier transform infrared spectrometer, by Jasco (3 mentions)
Jnm al500, by JEOL (3 mentions)
L 2420, by Hitachi (3 mentions)
Av 500, by Bruker (2 mentions)
Av 400, by Bruker (2 mentions)
Nicolet is5 ft ir spectrophotometer, by Thermo Fisher Scientific (2 mentions)
Kaleidagraph software, by Synergy Software (2 mentions)
Hypersil ods c18 column, by Thermo Fisher Scientific (2 mentions)
Dithiothreitol (dtt), by Merck Group (2 mentions)
L 2455 diode array detector, by Hitachi (2 mentions)
Lachrom elite hplc system, by Hitachi (2 mentions)
53 protocols using l 2130 pump
1
Spectroscopic Characterization of Novel Compounds
2
HPLC Analysis of SN38 Anticancer Drug
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SN38 was analyzed by high-performance liquid chromatography (HPLC). The HPLC system consisted of an L-2130 pump, L-2200 autosampler, L-7420 ultraviolet-visible detector at 265 nm (Hitachi, Tokyo, Japan), and a Purospher Star RP-18 column (250×4.6 mm, internal diameters 5 μm; Merck). The mobile phase was a mixture of 25 mM sodium dihydrogen phosphate (NaH2PO4, adjusted to pH 3.1 by 85% phosphoric acid) and acetonitrile (50:50 v:v), and the flow rate was 1 mL/min. Limits of detection and quantitation of SN38 were determined by dissolving SN38 at decreasing concentrations in distilled deionized water until the signal:noise ratios were 3 and 10, respectively. The linearity of the standard curves and intraday and interday precision and accuracy were established.
3
Comprehensive Analytical Characterization
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Example 2
General.
1H NMR spectrum was obtained on a Bruker AV400 or AV500 spectrometer using standard pulse programs. Melting point was recorded on a Fisher-Johns apparatus (uncorrected). MS data were measured on a JEOL JMX-HX110 mass spectrometer (HREIMS and HRFABMS), a JMS-SX102A mass spectrometer (EIMS and FABMS), or a Finnigan Mat 95S mass spectrometer (HRESIMS and ESIMS). TLC analyses were carried out on silica gel plates (KG60-F254, Merck). The microplate spectrophotometer Victor 2× (PerkinElmer, Fremont, Calif., USA) was used for fluorometric analysis. Unless otherwise mentioned, all chemicals and materials were used as received from commercial suppliers without further purification. CH2Cl2 was distilled from CaH under N2. THF was distilled from sodium and benzophenone under N2. All test compounds were estimated to be at least 98% pure as judged by HPLC analysis, which was performed on an Ascentis C18 column (150×4.6 mm) using an L-2130 pump (Hitachi) and a UV/Vis L-2420 detector (Hitachi) with UV detection at 250 nm.
4
HPLC Analysis of 734THIF Nanoparticles
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The HPLC analysis system (LaChrom Elite L-2000, Hitachi) consisted of an L-2130 pump, L-2200 autosampler, and L-2420 ultraviolet–visible (UV–vis) detector. The analysis was carried out using the Mightysil RP-18 GP column (250 × 4.6 mm id, 5 μm). The mobile phase consisted of acetonitrile and 10 mM KH2PO4 (35:65, v/v). The pH value was adjusted to 2.8 using phosphoric acid. The flow rate was set at 1.0 mL/min. The wavelength of the UV detector was set at 262 nm. The chromatogram of 734THIF is shown in Figure 2 , showing that the retention time for 734THIF appeared at 4.8 minutes. The 734THIF concentration of all nanoparticle samples was determined using a linear calibration curve (r=0.999) of 734THIF within the range of 0.01–50 μg/mL.
5
Multi-Angle Light Scattering Analysis
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All MALLS runs were performed using a S200 increase SEC column (10/300 GL, GE Healthcare). Sample injection and buffer flow was controlled by a Hitachi L2130 pump, following the SEC column was a L-2400 UV detector (Hitachi), Optilab T-rEX refractometer (Wyatt technologies) and a DAWN HELEOS-II multi angle light scattering detector (Wyatt technologies). Prior to injection, columns and systems were equilibrated in 5 to 10 column volumes of running buffer. 50 μL injections were performed using protein samples concentrated at a minimum of 2 mg.mL−1, constant flow rate of 0.5 mL.min−1 was used. Accurate MALLS mass prediction was performed with the Astra software (Wyatt Technologies). Curves were represented with Graphpad (Prism).
6
Quantitative Determination of Flavonoids and Limonoids
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The flavonoid standards used included: naringin, hesperidin, diosmin, quercetin, and hesperetin, which were prepared of methanol. The sample was conducted by reflux extraction with methanol for 2 h. The quantitative determination method of flavonoid composition was described previously [13 (link)]. The analysis method of limonoid quantitative determination was also described previously, as limonoid standards were dissolved in acetonitrile [14 (link)]. A 20 μL aliquots of filtrate were injected into a injection port and separated by an HPLC system (L-2130 pump and L-2400 UV detector, Hitachi, Tokyo, Japan) attached to RP-18GP250 column Mightysil (l = 250 mm; i.d. = 4.6 mm; thickness = 0.32 μm; Kanto Chemical Co., Inc., Tokyo, Japan). The calibration curves of each standard were established by plotting the peak area vs. corresponding concentration, respectively.
7
HPLC Analysis of Tocopherol in Oil Samples
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Each oil sample (0.1 g) was diluted with 2-propanol to a volume of 10 mL and filtered through an MS nylon syringe filter with a 0.45 μm pore size directly to vials and then immediately analyzed using an HPLC system. Aliquots of 10 µL of the filtrate were injected into the injection port and analyzed with HPLC (Hitachi L-2130 pump, Hitachi, Tokyo, Japan). The remaining procedures were carried out as previously reported [34 (link)] using an HPLC attached to a detector L-2400 UV and a Hitachi L-2130 pump. An RP-18GP250 Mightysil column (l = 250 mm; i.d. = 4.6 mm; thickness = 0.32 µm; Kanto Chemical Co., Inc., Tokyo, Japan) was used for separation. The same mobile phase and elution conditions were adopted. The calibration curves were, respectively, established for tocopherol by plotting the peak area vs. each corresponding concentration, from which quantitations of the standards were achieved.
8
Spectroscopic Characterization of Compounds
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The specific rotation values and IR spectra were measured using a JASCO P-2000 digital polarimeter and a THERMO Scientific Nicolet iS5 FT-IR spectrophotometer, respectively. ESIMS and HRESIMS were recorded using a BRUKER 7 Tesla solariX FTMS system. NMR spectra were obtained from a JEOL Resonance ECZ 400S or an ECZ 600R NMR spectrometer, with the residual signals of CHCl3 (δH 7.26 ppm) and CDCl3 (δC 77.0 ppm) used as the internal standards for 1H and 13C NMR, respectively. Coupling constants (J) are provided in Hz. Column chromatography was carried out with a silica gel (230~400 mesh, MERCK) column. Thin-layer chromatography was performed on plates precoated with silica gel 60 F254 (0.25-mm-thick, MERCK); the plates then sprayed with 10% (v/v) H2SO4 in methanol, followed by heating to visualize the spots. A normal-phase (NP) HPLC was performed using a system comprised of a HITACHI 5110 pump, a RHEODYNE 7725i injection port and a NP column (YMC pack SIL, 5 μm, 12 nm, 250 × 20 mm, YMC group). Reverse-phase (RP) HPLC was performed using a system comprised of a HITACHI L-2130 pump, a HITACHI L-2455 photodiode array detector, a RHEODYNE 7725i injection port and a RP column (Luna 5 µm C18(2) 100 Å, 250 × 21.2 mm, Phenomenex).
9
Solubility Screening for Microemulsion Formulation
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Solubility studies were conducted to select the appropriate components of ME formulation with high drug loading capacity. In the process, an excess amount of acacetin was separately added into 0.1 g of solvents such as ethanol, PEG300, PEG400; oils such as Lipoid MCT, mineral oil, IMP; surfactants like CrEL, Tween 80, Labra; co-surfactants like Trans. Then samples were vortexed for 5 min thoroughly mixed and followed by constantly shaken at 37 °C for 24 h to achieve the dissolution equilibrium. After that, the samples were centrifuged at 14 000 rpm for 30 min to precipitate the un-dissolved particles. The concentration of acacetin in the supernatants was qualified by HPLC analysis. All experiments were performed in triplicate.
The HPLC system was equipped with a C-18 column (Grace, 4.6 × 150 mm, 5 μm) and mobile phase was composed of a mixture of water and methanol (v/v, 3:7, with 0.2% phosphate acid) using an isocratic elution (Hitachi L2130 pump, L2200 Autosampler and L2400 Detector, Hitachi, Japan). The flow rate was 1.0 mL/min. The UV absorbance detector was set at 330 nm.
The HPLC system was equipped with a C-18 column (Grace, 4.6 × 150 mm, 5 μm) and mobile phase was composed of a mixture of water and methanol (v/v, 3:7, with 0.2% phosphate acid) using an isocratic elution (Hitachi L2130 pump, L2200 Autosampler and L2400 Detector, Hitachi, Japan). The flow rate was 1.0 mL/min. The UV absorbance detector was set at 330 nm.
10
Spectroscopic Analysis of Natural Compounds
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Optical rotation values were determined using a JASCO P-1020 polarimeter. UV spectra were recorded using a Hitachi U-2900 spectrometer. ECD spectra were acquired with a JASCO J-820 spectropolarimeter and IR spectra were recorded using a Shimadzu FTIR-8400S spectrophotometer. NMR spectra were acquired with a JEOL JNM-ECZ 400S spectrometer with tetramethylsilane as an internal standard. ESI–MS data were obtained using an Agilent 6230 LC/TOF mass spectrometer. HPLC was performed on a Hitachi HPLC system equipped with an L-2130 pump, an L-2200 autosampler, an L-2300 column oven, and an L-2455 diode array detector. Silica gel AP-300 (Toyota Kako), Sephadex LH-20 (GE Healthcare), and Cosmosil 75C18-OPN (Nacalai Tesque) were used for column chromatography (CC). Silica gel 60 F254 and RP-18 F254S (Merck) were used for TLC analysis.
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