Ready-SET-go! ELISA kits for IFN-γ, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17a and TGF-β1 (all from eBioscience) were used according to manufacturer’s instructions. IgE ELISA reagents (kind gift of Nicola Harris, EPFL), including IgE standard grown from TIB-141 (ATCC, Manassas, VA, USA), IgE unlabeled coating antibody grown from 6HD5, IgE biotin (Biolegend) and streptavidin-AP (Southern Biotech, Birmingham, AL, USA) were used as previously described34 (link).
Elisa kits for ifn γ
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ELISA kits for IFN-γ are laboratory tools designed to detect and quantify the presence of interferon-gamma (IFN-γ) in various sample types. These kits utilize the enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of IFN-γ in the tested samples.
Automatically generated - may contain errors
Lab products found in correlation
Ige biotin, by BioLegend (1 mentions)
Streptavidin ap, by Southern Biotech (1 mentions)
Anti cd28, by Thermo Fisher Scientific (1 mentions)
Il 17, by R&D Systems (1 mentions)
Anti cd3, by Thermo Fisher Scientific (1 mentions)
Gentamicin gm, by Merck Group (1 mentions)
Caspase 1 activity assay kit, by Beyotime (1 mentions)
5 protocols using elisa kits for ifn γ
1
Cytokine and IgE ELISA Protocols
2
Quantifying Lung Viral Titers and Cytokines
3
Measuring Th17 and CD4+ T Cell Cytokines
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To detect IL-17 produced by Th17 cells, cells were stimulated with 1 μg/mL anti-CD3 (eBioscience) and 1 μg/mL anti-CD28 (eBioscience) for 24 h. To detect other cytokines produced by the CD4+ T cells, cells were cultured with 1 μg/mL anti-CD3 and 1 μg/mL anti-CD28 for 8 h or 24 h. Culture supernatants were collected at the relevant times. To measure the concentration of cytokines in culture supernatants we used ELISA kits for IFN-γ (eBioscience) and IL-17 (R&D Systems) according to the manufacturers’ instructions. We analyzed the cytokine concentrations using microplate reader software (Thermo Fisher Scientific).
4
Cytokine Quantification in Cell Culture and Serum
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Cytokine levels in cell culture supernatants and in mouse serum were measured by enzyme-linked immunosorbent assay (ELISA). The ELISA kits for IFN-γ, IL-6, TNF-α, and IL-12p40 were purchased from eBioscience.
5
Immune Response Quantification Protocol
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GL was purchased from Sigma-Aldrich (purity ≥95.0%, St. Louis, MO, USA). Gentamicin (GM) was purchased from Sigma-Aldrich (St. Louis, MO, USA). ELISA kits for IFN-γ, IL-12p70, TNF-α, IL-6, and IL-10 were obtained from eBioscience (San Diego, CA, USA). Caspase-1 activity assay kit was purchased from Beyotime Institute of Biotechnology (Shanghai, China).
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