Paraffin or frozen 5 µm sections of skin tissue samples were stained with the following antibodies: CD31 antibody (1:100, ab28364 Abcam) for detection of endothelial cells; Calponin (1:100, EP798Y, Abcam) and α-SMA (1:200, ab5694, Abcam) for detection of perivascular cells, and F4/80 (1:100, ab6640, Abcam) for assessing macrophages in vivo. Collagen type I (1:200, ab21286, Abcam), collagen type IV (1:100, AB756, Chemicon), collagen type XVIII (1:100, sc-32720, Santa Cruz), collagen type VI (1:50, sc-167530, Santa Cruz), and fibronectin (1:500, sc-9068, Santa Cruz) were used for extracellular matrix and basement membrane evaluation. Incubation with primary antibody was followed by FITC or Texas Red labeled secondary antibody (1:500, Vector Laboratories). Nuclei were stained with Hoechst 33342 fluorescent dye (Thermo Scientific).
Sc 167530
Manufactured by Santa Cruz Biotechnology
Sc-167530 is a laboratory instrument designed for performing cell culture and tissue analysis. The core function of this equipment is to provide a controlled and sterile environment for culturing and maintaining cells or tissue samples. This product is intended to facilitate various cell-based assays and experiments in a research or clinical setting.
Automatically generated - may contain errors
Lab products found in correlation
Ep798y, by Abcam (2 mentions)
Ab756, by Merck Group (2 mentions)
Sc 32720, by Santa Cruz Biotechnology (2 mentions)
Sc 9068, by Santa Cruz Biotechnology (2 mentions)
Fitc or texas red labeled secondary antibody, by Vector Laboratories (2 mentions)
Ab28364, by Abcam (2 mentions)
Ab6640, by Abcam (2 mentions)
Hoechst 33342 fluorescent dye, by Thermo Fisher Scientific (2 mentions)
Ab21286, by Abcam (2 mentions)
Ab5694, by Abcam (2 mentions)
2 protocols using sc 167530
1
Comprehensive Immunohistochemistry Protocol for Skin Tissue
2
Comprehensive Immunohistochemistry Protocol for Skin Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Paraffin or frozen 5 µm sections of skin tissue samples were stained with the following antibodies: CD31 antibody (1:100, ab28364 Abcam) for detection of endothelial cells; Calponin (1:100, EP798Y, Abcam) and α-SMA (1:200, ab5694, Abcam) for detection of perivascular cells, and F4/80 (1:100, ab6640, Abcam) for assessing macrophages in vivo. Collagen type I (1:200, ab21286, Abcam), collagen type IV (1:100, AB756, Chemicon), collagen type XVIII (1:100, sc-32720, Santa Cruz), collagen type VI (1:50, sc-167530, Santa Cruz), and fibronectin (1:500, sc-9068, Santa Cruz) were used for extracellular matrix and basement membrane evaluation. Incubation with primary antibody was followed by FITC or Texas Red labeled secondary antibody (1:500, Vector Laboratories). Nuclei were stained with Hoechst 33342 fluorescent dye (Thermo Scientific).
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