Sch B and Rhodamine 123 (Rho 123) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sch B was dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich) to create a 100 mM stock solution. The stock solution was further diluted with culture media to acquire the desired concentrations. The control groups were treated with equal volumes of DMSO. Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Tokyo, Japan). The Annexin V/PI apoptosis kit was obtained from BD Biosciences (San Diego, CA, USA). Primary antibodies against cleaved PARP, cleaved caspase-3, CDK4, Bcl-2, Bax, cleaved caspase-9 and cyclin D1, and secondary antibodies (goat anti-rabbit) were all purchased from Cell Signaling Technology (Danvers, MA, USA). β-actin was purchased from Abways Technology (Shanghai, China).
Rhodamine 123 rho 123
Manufactured by Merck Group
Sourced in United States, Australia
Rhodamine 123 (Rho 123) is a fluorescent dye commonly used in laboratory applications. It is a cationic dye that selectively accumulates in the mitochondria of living cells, making it a useful tool for studying mitochondrial function and membrane potential.
Automatically generated - may contain errors
Lab products found in correlation
Doxorubicin, by Merck Group (5 mentions)
Paclitaxel, by Merck Group (4 mentions)
Verapamil, by Merck Group (3 mentions)
Hoechst 33342, by Merck Group (3 mentions)
Topotecan, by Merck Group (3 mentions)
Mitoxantrone, by Merck Group (2 mentions)
Sybr green qpcr master mix, by ExCell Bio (2 mentions)
Vincristine, by Merck Group (2 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (2 mentions)
Fumitremorgin c ftc, by Merck Group (2 mentions)
Cisplatin, by Merck Group (2 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Facscalibur, by BD (2 mentions)
β actin, by Abways (1 mentions)
Annexin 5 pi apoptosis kit, by BD (1 mentions)
Primary antibodies against cleaved parp, by Cell Signaling Technology (1 mentions)
Secondary antibodies goat anti rabbit, by Cell Signaling Technology (1 mentions)
29 protocols using rhodamine 123 rho 123
1
Sch B Induces Apoptosis in Cancer Cells
2
Acquisition of Analytical-Grade Compounds
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The following compounds were obtained from Sigma-Aldrich (Stockholm, Sweden): atenolol, carbamazepine (Cbz), loperamide, propranolol, rhodamine 123 (Rho123), quinidine, and verapamil. Cyclosporin A (CsA; Sandimmun, 50 mg/mL, Novartis, Tärby, Sweden) was purchased from the drug store, Apoteket AB (Lund, Sweden). All other chemicals were analytical reagent grade and were purchased from Sigma-Aldrich.
3
Salmonella-Mediated Cancer Therapy
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Salmonella enterica serovar choleraesuis (S. Choleraesuis; S.C.) (ATCC 15480) was obtained from Bioresources Collection and Research Center (Hsinchu, Taiwan) 10 (link). B16F10 (mouse melanoma) 11 (link) and 4T1 (mouse breast cancer) 12 (link) cells were maintained in culture dish with Dulbecco's Modified Eagle Medium containing 1% antibiotics (100 units/mL penicillin and 100 μg/mL streptomycin), 2mM l-glutamine and 10% fetal bovine serum. Rhodamine 123 (Rho-123) and 5-Fluorouracil (5-FU) were purchased from Sigma Aldrich. Constitutively active AKT plasmid was kindly provided by Dr. Chiau-Yuang Tsai (Department of molecular immunology, Osaka University) 13 (link). Cells were treated with plasmids for 16 h in serum free medium prior to adding Salmonella (multiplicity of infection (MOI) = 10) into cells for 90 min. C57BL/6 (B16F10 mouse tumor model) and BALB/c (4T1 mouse tumor model) female mice were purchased from the National Laboratory Animal Center of Taiwan. The animals were maintained in a pathogen-free animal care facility in isothermal conditions with regular photoperiods. The experimental protocol adhered to the rules of the Animal Protection Act of Taiwan and was approved by the Laboratory Animal Care and Use Committee of the National Sun Yat-sen University.
4
Rhodamine-123 Uptake Assay for BMDMs
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BMDMs or Abcb1b–/– clones were seeded into 96-well plates at a concentration of 1 × 105 cells per well. Where indicated, BMDMs were treated with elacridar overnight (2–10 μM). Rhodamine-123 (Rho123; 2 μM, Sigma) was added, and cells were incubated at 37° C for 30 min. Cells were then washed with PBS three times to remove any extracellular Rho123, and DMEM were replaced, after which cells were incubated for a further 30 min at 37° C. Cells were then washed again with PBS and lysed in cell lysis buffer before being read at excitation and emission wavelengths of 485 and 535 nm, respectively, in a fluorescent plate reader.
5
Pharmacokinetics of LZD in Rats
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LZD injection solution (Zyvox Injection, 600 mg) used for intravenous administration and tablets (Zyvox Tablets, 600 mg) used for oral administration in rats were purchased from Pfizer Japan, Inc. (Tokyo, Japan). RFP, Lucifer yellow (LY) and rhodamine 123 (Rho123) were purchased from Sigma-Aldrich (Tokyo, Japan). Pentobarbital and diethyl ether were purchased from Nakalai Tesque, Inc. (Kyoto, Japan). All other chemicals used were of analytical or high performance liquid chromatography (HPLC) grade.
6
Isolation and Characterization of Rat Notochordal Progenitor Cells
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24 healthy male Sprague-Dawley (SD) rats with an average body weight (BW) of 362±35 g were selected as NP cell donors (obtained from the Center of Experimental Animals of Wuhan University, Wuhan, China). Dulbecco’s modified Eagle’s medium/Ham’s F-12 (DMEM/F-12) was obtained from Gibco Life Technologies (Carlsbad, CA, USA) and fetal bovine serum (FBS) was obtained from HyClone (Logan, UT, USA). Carboxymethylated chitosan (CMCS, purity >99%) was supplied by the Institute of Chemistry and Environmental Science of Wuhan University. A cell counting kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). Primers were provided by Invitrogen Life Technologies (Carlsbad, CA, USA). Rabbit polyclonal anti-Bcl-2 (#2876) and rabbit monoclonal anti-β-actin (13E5; #4970) antibodies were obtained from Cell Signaling Technology, Inc. (Beverly, MA, USA). The anti-inducible nitric oxide synthase rabbit polyclonal (iNOS; sc-651) antibody was from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Rhodamine 123 (Rho123) and Hoechst 33342 were obtained from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline (PBS, ×10, ST476) and SDS-PAGE Gel Kit (P0012A), were obtained from the Beyotime Institute of Biotechnology (Haimen, China) and were of the highest purity commercially available.
7
Investigating Multidrug Resistance Mechanisms
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Lazertinib was purchased from MedChemExpress (Princeton, NJ, USA). Vincristine (VCR), paclitaxel, DOX, mitoxantrone (MX), topotecan, G418, fumitremorgin C (FTC), verapamil (VRP), cisplatin, rhodamine 123 (Rho 123), and 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan (MTT) were acquired from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against ABCB1, ABCG2, ERK, and p-ERK, Akt, p-Akt, and flow cytometry antibodies against ABCG2 were obtained from Santa Cruz Biotechnology Inc. (Paso Robles, CA, USA). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was obtained from Kangchen Co. (Shanghai, China). ABCB1 and mouse IgG2b/κ antibodies used in flow cytometry were obtained from BD Biosciences (San Jose, CA, USA). RPMI 1640 and DMEM media were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). SYBR Green qPCR Master Mix was obtained from ExCell Bio (Shanghai, China).
8
Cytotoxicity Evaluation of Sesquiterpenes
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McCoy’s modified medium, RPMI-1640, N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES) buffer, sodium pyruvate, d -glutamine, resazurin, rhodamine 123 (RHO 123) and neutral red were supplied by Sigma-Aldrich (Prague, Czech Republic). Fetal bovine serum and gentamicin sulfate were purchased from Invitrogen (Carlsbad, CA, USA) and bovine serum albumin (BSA) from Fluka (Prague, Czech Republic). Doxorubicin and pure sesquiterpenes (α-humulene, β-caryophyllene oxide, trans-nerolidol, valencene) were supplied by Sigma-Aldrich. Stock solutions of sesquiterpenes were prepared in dimethyl sulfoxide (DMSO) and stored in the dark at 4 °C. All other chemicals used were of HPLC or analytical grade and provided by Sigma-Aldrich.
9
Cytotoxicity Evaluation of Doxorubicin and Lapatinib
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Doxorubicin (Dox), paclitaxel, verapamil, 3-(4,5-dimethylthiazol-yl)-2,5-diphenylLapatinibrazolium bromide (MTT) and rhodamine 123 (Rho 123) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Lapatinib was purchased from LC laboratories (Woburn, MA, USA). Dulbecco's modified Eagle's medium (DMEM) were products of Gibco BRL (Grand Island, NY, USA). Monoclonal antibodies against ABCB1 were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, CA, USA). Other routine laboratory reagents were of analytical grade obtained from various commercial sources (Guangzhou, China).
10
Anlotinib Enhances Multidrug Resistance Inhibition
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Anlotinib was obtained from Chia Tai Tianqing Pharmaceutical Group Co., Ltd. (Nanjing, China). All cell culture reagents were purchased from Invitrogen Life Technologies (Carlsbad, CA, United States). Antibodies specific for P-gp/ABCB1 and GAPDH were purchased from Cell Signaling Technology, Inc. (Danvers, MA, United States). Dimethyl sulfoxide (DMSO), DOX, paclitaxel, vincristine, rhodamine 123 (Rho-123), and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, United States).
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