Xylazil

Mice were anaesthetized with a mixture of ketamine (8.7 mg/ml; Mavlab) and xylazil (2 mg/ml; Troy Laboratories Pty Ltd) and placed in a stereotaxic apparatus (Kopf Instruments). Mice were then injected with 2 μl of 5 μg/μl of LPS, or vehicle control, at a rate of 0.5 μl/minute in the substantia nigra at the following coordinates relative to bregma: AP -3.0, ML +1.3, DV -4.7. All mice were sacrificed 15 days after lesioning.

Female human HER‐2 TG mice were anaesthetised with Ketamine (100 mg/kg) and Xylazil (10 mg/kg) (Troy Laboratories) and injected orthotopically with 2 × 10⁵ HER‐2‐E0771 cells resuspended in 20 μl D‐PBS into the fourth mammary fat pad. At day 6 post‐tumour cell injection, human HER‐2 TG mice were pre‐conditioned with total body irradiation (4 Gy) prior to the adoptive transfer of 6 × 10⁶ FACS‐purified CD8⁺CD44^hiCD62L^hi CAR T cells. Mice were treated with 50,000 IU IL‐2 on days 0–4 after T‐cell transfer.

After 16 weeks of the diets, rats were anaesthetised by intraperitoneal injection of Zoletil (tiletamine 15 mg/kg, zolazepam 15 mg/kg; Virbac, Peakhurst, NSW, Australia) and Xylazil (xylazine 10 mg/kg; Troy Laboratories, Smithfield, NSW, Australia) and partial medial meniscectomy of the right knee was performed [18 (link)]. The separate group of sham-operated rats was subjected to a similar surgical procedure in the right knee, except that the ligament and meniscus were not excised or manipulated. After the conclusion of the surgery, all rats received buprenorphine 0.05 mg/kg and gentamicin 5 mg/kg. Rats were allowed to recover for 1 week, during which time they were placed on a standard rat chow diet and given post-surgical medications. Once rats had recovered, they were placed on the same diet as before the surgery for a further period of 8 weeks.

Full-field scotopic electroretinography (ERG) was performed to assess the retinal function of dim-reared controls and animals after 5 days photo-oxidative damage, as well as injected mice as previously described^{14 (link)}. Mice were dark-adapted overnight before being anaesthetised with an intraperitoneal injection of Ketamine (100 mg/kg; Troy Laboratories, NSW, Australia) and Xylazil (10 mg/kg; Troy Laboratories, NSW, Australia). Both pupils were dilated with one drop each of 2.5% w/v Phenylephrine hydrochloride and 1% w/v Tropicamide (Bausch and Lomb, NY, USA). A single- or twin-flash paradigm was used to elicit a mixed response from rods and cones, and an isolated cone response, respectively. Flash stimuli for mixed responses were provided by an LED-based system (FS-250A Enhanced Ganzfeld, Photometric Solutions International, VIC, AUS), over a stimulus intensity range of 6.3 log cd s m⁻² (range −4.4–1.9 log cd s m⁻²). Amplitudes of the a-wave and b-wave were analysed using LabChart 8 software (AD Instruments, Dunedin, NZ) and data were expressed as the mean wave amplitude ± SEM (μV).

Mice were anaesthetized with a mixture of ketamine (100 mg/kg) and xylazil (10 mg/kg) (Troy Laboratories) administered intraperitoneally (10 mL/g body weight).
Refresh®Lacri-Lube® (Allergan) was applied to mouse eyes for the duration of anaesthesia. The flank of the mouse was shaved and depilated with Veet®(Reckitt Benckiser). A small (~2 mm²) area of surface skin on the flank was abraded using a MultiPro Dremel with a grindstone attachment on low speed. B16 melanoma cells (10⁵) were washed, resuspended in 10 μL of Matrigel™ (BD), applied to the abraded area and allowed to set. To contain the cells, the abraded site was covered with a piece of Op-site FlexigridTM (Smith and Nephew), then the torsos of the mice were wrapped, first with a soft hypoallergenic MicroporeTM tape (3 M Health Care) and then a second layer of Fixomull Stretch (BSN Healthcare). Mice recovered from anaesthesia on a heating pad and were monitored daily until bandages were removed 5 days post-grafting. Mice with tumours >1000 mm³ were euthanised. Tumour-free mice were defined as mice with no palpable masses.