Epirubicin was commercially available (Sigma, St. Louis, MO, USA). Gold nanoparticles were prepared with gold chloride and sodium citrate (Sinopharm Chemical Reagent Co., Ltd.). RPMI-1640 medium, L-glutamine, penicillin and streptomycin (Hyclone, USA) were purchased. M199 medium, fetal bovine serum, and trypsin (Gibco Le.) were commercially available. Human hepatocellular carcinoma cell line HepG2 was maintained in our own laboratory. Human umbilical vein endothelial cells (HUVECs) were obtained by primary isolation and culture[7 (link)]. Ultraviolet-visible (UV-Vis) spectrophotometer (Lambda 45, PerkinElmer, USA) and transmission electron microscope (TECNAI 10, Philips Ltd.), full wavelength multifunctional micro-plate reader (Safire II, Bio-Rad, Hercules, CA, USA), atomic force microscopy (American Thermo-microscope, Ltd.) and micro-titer plates (Costar) were used for the in vitro study.
Gold chloride
Manufactured by Sinopharm
Sourced in China
Gold chloride is a chemical compound with the formula AuCl3. It is a yellow crystalline solid that is used as a precursor in various chemical and industrial processes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using gold chloride
1
Epirubicin-Loaded Gold Nanoparticles
2
Fontana-Masson Staining for Melanin
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Fontana–Masson staining was carried out to visualize melanin content and distribution in irradiated dorsal skin as described previously [12 (link)]. Sections were deparaffinized and hydrated in water and placedinto a 2.5% silver nitrate solution (Sinopharm Chemical Reagent Co., Ltd, Beijing, China) for 4 h in a dark chamber. After rinsing in water, the slides were placed in 0.1% gold chloride (Sinopharm Chemical Reagent Co., Ltd, Beijing, China) for 1 min,rinsed again in water, and then in 5% sodium thiosulfate (Sinopharm Chemical Reagent Co., Ltd, Beijing, China) for an additional2 min. After a final rinse with water, the sections were counter-stained with eosin red.
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