Optical rotations were measured using a 341 Polarimeter (Perkin-kinelmer, Inc., Norwalk, CT, USA). The CD spectra were measured on a Chirascan circular dichroism spectrometer (Applied Photophysics, Ltd., Surrey, UK). UV spectra were measured on a U-2900 spectrophotometer (Hitachi, Tokyo, Japan). IR spectra were recorded on an Affinity-1 FT-IR spectrometer (Shimadzu, Tokyo, Japan). The 1D and 2D NMR spectra were recorded on a Bruker AV-700 MHz NMR spectrometer (Bruker Biospin GmbH, Rheinstetten, Germany) with tetramethylsilane (TMS) as the internal standard. Mass spectrometric data were obtained on a quadrupole-time-of-flight mass spectrometry (Bruker Maxis 4G) for HRESIMS. Column chromatography was performed while using silica gel (100–200 mesh, 300–400 mesh; Jiangyou Silica gel development, Inc., Yantai, China), Sephadex LH-20 (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). HPLC was carried out while using a reversed-phase column (Phenomenex Gemini C18, 250 mm × 4.6 mm, 5 μm; Phenomenex, Torrance, CA, USA) with UV detection at 270 nm and 320 nm. Semi-preparative HPLC was performed on a Hitachi HPLC station (Hitachi-L2130) with a Diode Array Detector (Hitachi L-2455) using a Phenomenex ODS column (250 mm × 10.0 mm, 5 mm; Phenomenex, Torrance, CA, USA) with UV detection at 320 nm.
Affinity 1 ft ir spectrometer
Manufactured by Shimadzu
Sourced in Japan
The Affinity-1 FT-IR spectrometer is a laboratory instrument designed for the analysis of molecular structures. It uses Fourier transform infrared spectroscopy to measure the absorption of infrared light by a sample, providing information about the chemical composition and functional groups present. The Affinity-1 is capable of analyzing a wide range of sample types, including solids, liquids, and gases.
Automatically generated - may contain errors
Lab products found in correlation
Maxis 4g, by Bruker (3 mentions)
Gemini c18, by Phenomenex (2 mentions)
L 2455, by Phenomenex (2 mentions)
Ods column, by Phenomenex (2 mentions)
Chirascan circular dichroism spectrometer, by Applied Photophysics (2 mentions)
U 2900 spectrophotometer, by Hitachi (2 mentions)
Av 700 mhz nmr spectrometer, by Bruker (2 mentions)
341 polarimeter, by PerkinElmer (2 mentions)
Sephadex lh 20, by Cytiva (1 mentions)
Uv 2600 spectrophotometer, by Shimadzu (1 mentions)
1260 infinity hplc, by Agilent Technologies (1 mentions)
Avance 2 400 spectrometer, by Bruker (1 mentions)
Accutof jms t100lc, by JEOL (1 mentions)
Gcms qp2010, by Shimadzu (1 mentions)
4 protocols using affinity 1 ft ir spectrometer
1
Comprehensive Analytical Characterization
2
Comprehensive Characterization of Compounds
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Optical rotations were measured using a 341 Polarimeter (PerkinElmer, Inc., Norwalk, CT, USA). The CD spectra were measured on a Chirascan circular dichroism spectrometer (Applied Photophysics, Ltd., Surrey, UK). UV spectra were recorded on UV-2600 spectrophotometer (Shimadzu). IR spectra were recorded on Affinity-1 FT-IR spectrometer (Shimadzu). NMR spectra were recorded on a AMC instrument (Bruker Biosciences Corporation, USA) operating at 700 MHz (1H-NMR) or 175 MHz (13C-NMR), respectively. Mass spectrometric data were obtained on a time-of-flight mass spectrometry (Bruker maxis 4G) for HRESIMS. Materials for column chromatography (CC) were silica gel (100–200 mesh; 300–400 mesh; Jiangyou Silica gel development Inc.), Sephadex LH-20 (40–70 μm; Amersham Pharmacia Biotech), and YMC*GEL ODS-A (12 nm S-50 μm; YMC Company Ltd.). Thin-Layer-Chromatography (TLC, 0.1–0.2 mm or 0.3–0.4 mm) was conducted with precoated glass plates (silica gel GF254, 10–40 nm, Jiangyou Silica gel development, Inc.). Medium pressure liquid chromatography (MPLC) was performed on CHEETAH flash system (Bonna-Agela technologies Inc.). Compounds were detected with a 1260 Infinity HPLC (Agilent Inc.). Semi-preparative HPLC was carried out on Gemini C18 column (5 μm; Phenomenex Company Ltd.) except for specific indication.
3
Synthesis and Characterization of Benzimidazole and Imine
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All chemical reagents used for the synthesis of benzimidazole and subsequent imine were used as received and without further purification. Elemental analyses were performed using Flash EA 1112 Series CHN Analyzer. A Shimadzu Affinity 1 FT-IR spectrometer was used to obtain the infrared spectra. IR data are reported using the following abbreviations: vs = very strong; s = strong; m = medium; w = weak; sh = shoulder; br = broad. 1H and 13C{1H} NMR spectra were obtained on a Bruker Avance II 400 spectrometer using DMSO-d6 as a solvent at 25 °C. The following abbreviations were used: s = singlet; d = doublet; t = triplet; m = multiplet. The mass spectrum of the benzimidazole was recorded on a Shimadzu-GCMS-QP2010 at 70 eV by electronic impact (EI) ionization, while the mass spectrum of the derived imine was obtained by direct analysis in real time (DART) ionization system on a JEOL AccuTOF JMS-T100LC.
4
Spectroscopic Characterization of Compounds
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Optical rotations were measured using a 341 Polarimeter (Perkin-Elmer, Inc., Norwalk, CT, USA). UV spectra were measured on a U-2900 spectrophotometer (Hitachi, Tokyo, Japan). IR spectra were recorded on an Affinity-1 FT-IR spectrometer (Shimadzu, Tokyo, Japan). Melting points were measured using an SGW®X-5 micro melting point meter (Shanghai INESA Physico optiacal Instrument Co., Ltd., Shanghai, China). 1D and 2D NMR spectra were recorded on a Bruker AV-700 MHz NMR spectrometer (Bruker Biospin GmbH, Rheinstetten, Germany) with tetramethylsilane (TMS) as the internal standard. Mass spectrometric data were obtained on a quadrupole-time-of-flight mass spectrometer (Bruker Maxis 4G) for HRESIMS. Column chromatography was performed using silica gel (100–200 mesh, 300–400 mesh; Jiangyou Silica gel development, Inc., Yantai, China), Sephadex LH-20 (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). HPLC was carried out while a reversed-phase column (Phenomenex Gemini C18, 250 mm × 4.6 mm, 5 μm; Phenomenex, Torrance, CA, USA) with UV detection at 254 nm and 360 nm. Semi-preparative HPLC was performed on a Hitachi HPLC station (Hitachi-L2130) with a Diode Array Detector (Hitachi L-2455) using a Phenomenex ODS column (250 mm × 10.0 mm, 5 μm; Phenomenex, Torrance, CA, USA) with UV detection at 360 nm.
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