The SOD levels in rat testicular tissue were measured using a colorimetric SOD activity assay kit (Sigma-Aldrich, Darmstadt, Germany) at a wavelength of 550 nm [18 (link)]. Blood samples were collected from the aortic arch to obtain serum for measuring the MDA and testosterone levels. The MDA levels in serum were measured using the thiobarbituric acid-reactive substance (Sigma-Aldrich) method with an ultraviolet-1601 spectrophotometer at a maximum wavelength of 535 nm [19 ]. Serum testosterone was measured using a solid-phase competitive chemiluminescence enzyme immunoassay with a testosterone kit (IMMULITE® 1000 total testosterone, Siemens AG, Munich, Germany) [20 (link)].
Colorimetric sod activity assay kit
Manufactured by Merck Group
Sourced in Germany, United States
The Colorimetric SOD Activity Assay Kit is a laboratory equipment product that measures the activity of superoxide dismutase (SOD) enzymes. It uses a colorimetric method to quantify SOD activity in various sample types.
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Lab products found in correlation
3 protocols using colorimetric sod activity assay kit
1
Oxidative Stress and Testosterone Levels
2
Antioxidant Biomarker Analysis
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The antioxidant biomarkers SOD and GSH were investigated using a colorimetric SOD activity assay kit (Sigma-Aldrich, USA) and a GSH + GSSG/GSH assay kit (Abcam, USA) according to the manufacturer's instructions, respectively.
3
Superoxide Generation and SOD Activity
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Cells in 96-well plates were refed before treatment with 100 μL of a 70% Hank's Buffered Solution/30% RPMI mix to limit potential antioxidant effects of complete medium. A superoxide generating hypoxanthine (X) and xanthine oxidase (XO) (1.0 mU/mL) combination was added to cells and L3000/SOD3 complexes were added after 1 h. Supernatant was collected after 48 h and SOD activity assessed using a colorimetric SOD activity assay kit (Sigma-Aldrich) (n = 3).
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