Bnp elisa kit

BNP (natriuretic peptide type B) is generally accepted as assessment marker for heart failure. In this study, we collected blood from ventricle of mice that were killed by cervical dislocation. A total of 0.5 ml serum was gained from mice blood and equivalent buffer was added. The specific procedures were carried out following the protocol of BNP ELISA kit (RAB0386, minimum detectable concentration is 1.66 pg/mL, detection range, is 0.1–1,000 pg/mL; Sigma-Aldrich, St. Louis, MO, USA) and absorbance value at 450 nm were detected by microplate reader.

Brain natriuretic peptide (BNP), a biomarker of congestive heart failure, is clinically used to assess the severity of heart dysfunction. Cardiac troponin I (cTNI), myoglobin and creatine kinase-MB (CK-MB) are biomarkers of myocardial injury. At the end of the follow-up period, the rats were anesthetized using chloral hydrate (300 mg/kg, i.p.), and their body weights were measured. They were subsequently placed on a warm platform. Blood samples were collected via the tail vein and centrifuged to obtain serum. The latter was stored at -80°C until needed. The serum concentrations of BNP, cTNI, myoglobin and CK-MB were detected using ELISA kits in accordance with the manufacturers’ instructions (BNP Elisa Kit: Sigma, Saint Louis, USA; cTNI Elisa Kit: Guduo, Shanghai, China; Myoglobin Elisa Kit: Xinyu, Shanghai, China; CK-MB Elisa Kit: Uscnk, Wuhan, China).

The brain natriuretic peptide (BNP) is a biomarker of CHF used to determine the severity of the condition. The serum concentration of BNP was detected using ELISA kits in accordance with the manufacturers’ instructions (BNP Elisa Kit: Sigma, Saint Louis, United States).