Human aortic smooth muscle cells were purchased from Lonza (Allendale, NJ) and maintained in smooth muscle basal medium supplemented with 5% fetal bovine serum (FBS), hEGF, insulin, hFGF-B, and gentamicin/amphotericin-B (SmGM-2 SingleQuots; Lonza), as per the manufacturer's instructions, and rat aortic smooth muscle cells were purchased from Lonza and maintained in Dulbecco's modified Eagle medium supplemented with 10% FBS. Cells were grown to 60%–70% confluence, serum-deprived for at least 12 h, pretreated with 0.1–10 nM Ex-4 or saline for 12 h, and stimulated with FBS at a final concentration of 10%. Cells were used between passages three and six for the experiments, and individual experiments were repeated at least three times with different preparations of cells.
Rat aortic smooth muscle cells
Manufactured by Lonza
Sourced in Switzerland
Rat aortic smooth muscle cells are primary cell cultures derived from the aortic smooth muscle tissue of rats. They are used for in vitro studies of vascular biology and function.
Automatically generated - may contain errors
2 protocols using rat aortic smooth muscle cells
1
Exendin-4 Modulates Aortic Smooth Muscle
2
Co-culture of Aortic Smooth Muscle and Endothelial Cells
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