Sod elisa kit

Blood of three fish from each tank was extracted from the caudal fin after the feeding trial. Serum from the blood samples was separated by centrifugation at 2,716 × g at 4°C for 10 min and kept at –70°C. The turbidimetric assay for lysozyme activity was performed based on Lange et al.'s [36 (link)] study. Superoxide dismutase (SOD) activity was measured using a SOD ELISA kit (MyBioSource, mbs705758) to evaluate the ability of the test solution to inhibit the reaction of superoxide. The same methods and procedures for measuring lysozyme and SOD activities of the fish were used in Jeong et al.'s [32 (link)] study.

Blood of three fish from each tank was extracted from the caudal fin after the feeding trial. Serum from the blood samples was separated by centrifugation at 2,716 × g at 4°C for 10 min and kept at –70°C. The turbidimetric assay for lysozyme activity was performed based on Lange et al.'s [36 (link)] study. Superoxide dismutase (SOD) activity was measured using a SOD ELISA kit (MyBioSource, mbs705758) to evaluate the ability of the test solution to inhibit the reaction of superoxide. The same methods and procedures for measuring lysozyme and SOD activities of the fish were used in Jeong et al.'s [32 (link)] study.

All kits used for this part were rat-specific. MDA ELISA kit for rats was used to measure levels of malondialdehyde (MDA) in the liver homogenates (#MBS268427, MyBioSource, CA, USA). GSH ELISA kit (#MBS265966; MyBioSource, CA, USA), SOD ELISA kit (#MBS036924, MyBioSource, CA, USA), CAT ELISA kit (#MBS726781, MyBioSource, CA, USA), IL-6 ELISA kit (#MBS269892, MyBioSource, CA, USA), HO-1 ELISA kit (MBS764989, MyBioSource, CA, USA) and TNF-αELISA kits (#MBS2507393, MyBioSource, CA, USA) were used to measure the levels of glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the liver homogenates. G6PC ELISA kit (#MBS097902 MyBioSource, CA, USA), FBP1 ELISA kit (#MBS931493, MyBioSource, CA, USA), and GLY ELISA kit (#MBS1600418; MyBioSource, CA, USA) were used to measure the levels of glucose-6 phosphatase (G-6-Pase), fructose-1,6-bisphosphatase 1 (PBP1), and glycogen in the liver homogenates. The TransAM Nrf2, NF-E2-related factor 2 (Nrf2 DNA binding ELISA) kit (#50296, ActiveMotif, Tokyo, Japan) and TransAM NF-κB p65 ELISA kits (#40096, ActiveMotif, Tokyo, Japan) were used to measure the total and cytoplasmic levels of Nrf2 and NF-κB p65 in the homogenates. All procedures were conducted according to each manufacturer’s instructions for 8 samples/groups.