Mice (Ldlr -/-and littermates Ldlr -/-IAP Tg ) maintained on standard rodent chow diet were used for these studies. After an overnight fast and collection of a baseline (0 hour) blood sample, mice were given tyloxapol (500 mg/kg) intravenously to block lipoprotein lipase and clearance of plasma lipids. After 5 minutes, mice were gavaged with corn oil (200 µL), and tail blood samples were collected at 1, 2, and 3 hours. Total triglyceride (TG) levels in these plasma samples were determined using L-Type TG M kit from Wako Diagnostics. 24 (link) To determine the effects of LPS on intestinal lipid absorption or plasma lipids, C57BL/6 mice were given an intraperitoneal injection of LPS (0.25 µg/g) followed by intravenous injection of tyloxapol (500 mg/kg). After 5 minutes, mice were gavaged with corn oil (200 ul) and blood was collected by cardiac puncture at the time of necropsy after 3 hours. Level of plasma TGs (mg/dl) was determined using L-Type TG M kit from Wako Diagnostics.
L type tg m kit
Manufactured by Fujifilm
Sourced in Japan
The L-Type TG M kit is a laboratory equipment product offered by Fujifilm. It serves as a core function for its intended use, but a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
Lab products found in correlation
Nefa c test kit, by Fujifilm (2 mentions)
Cholesterol reagent, by Horiba (2 mentions)
Liquid glucose oxidase reagent set, by Horiba (2 mentions)
Liquid alt sgpt reagent set, by Horiba (2 mentions)
Hdl cholesterol precipitating reagent set, by Horiba (2 mentions)
Glycerol colorimetric assay kit, by Cayman Chemical (1 mentions)
Cholesterol reagent set, by Horiba (1 mentions)
Nefa hr 2 kit, by Fujifilm (1 mentions)
Freestyle blood glucose monitoring system, by Nipro (1 mentions)
9 protocols using l type tg m kit
1
Intestinal Lipid Absorption and Plasma Lipids
2
Lipid Quantification in Frozen Tissues
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Frozen liver and WAT were thawed, homogenized in PBS, and mixed with a solution of chloroform/methanol (2:1). The mixture was swirled on a rotating at 4 °C for 12 h, and centrifuged at 3,000 x g for 15 min. The lower layer was obtained and evaporated at 40 °C to dryness under a stream of nitrogen gas. The residue was dissolved in ethanol, and tissue contents of TC, TG, NEFA and glycerol were measured using Determiner L TC II kit (Kyowa Medex, Tokyo, Japan), L-Type TG M kit (Wako, Osaka, Japan), NEFA C-test kit (Wako, Osaka, Japan) and glycerol colorimetric assay kits (Cayman chemical, Michigan, USA), respectively, following the manufacturer’s instructions.
3
Liver Lipid Extraction and Serum Analysis
4
Enzymatic Measurement of Plasma Lipid Levels
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Circulating concentrations of TC and triglyceride (TG) were enzymatically measured using L-Type TG M kit (Wako, Richmond, VA, USA) and Cholesterol reagent set (Pointe Scientific, Canton, MI, USA), respectively, as we previously described [25 (link)]. Plasma concentrations of high-density lipoprotein cholesterol (HDL-C) were measured using the Cholesterol reagent set after precipitating apoB containing lipoproteins using HDL cholesterol precipitating reagent set (Pointe Scientific, Canton, MI, USA).
5
Liver Triglyceride Extraction and Measurement
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Liver triglycerides were extracted as previously described (2 (link)) and measured by Wako L-Type TG M kit.
6
Metabolic Biomarker Profiling in Animals
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Blood samples were collected from animals at the time of sacrifice (~24 weeks of age), and metabolic biomarker levels were measured using commercially available enzyme-linked immunosorbent assay (ELISA) kits for plasma insulin and adiponectin (EMD Millipore, Billerica, MA) and a fluorimetric assay for total serum cholesterol (Cayman Chemical, Ann Arbor, MI). The L-type TG M kit and NEFA-HR(2) kit (Wako Diagnostics, Richmond, VA), which utilize an enzymatic colorimetric method, were used to measure serum triglycerides and free fatty acids, respectively.
7
Blood Glucose and Lipid Levels
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Blood glucose levels were measured with a free style blood glucose monitoring system (NIPRO, Osaka, Japan). Plasma total cholesterol (TC) was measured using Determiner L TC II kit (Kyowa Medex, Tokyo, Japan). Plasma triglyceride (TG) and non-esterified fatty acid (NEFA) levels were measured using L-Type TG M kit and NEFA C-test kit (Wako, Osaka, Japan).
8
Liver Triglyceride Quantification Protocol
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Liver triglycerides were extracted and quantified as follows: liver tissue was homogenized in a 5% NP-40 solution. Samples were heated to 95°C for five minutes, cooled on ice, and then subsequently heated to 95°C for another five minute incubation. Samples were spun at 14,000 rpm in a room temperature centrifuge, and supernatants were used to quantify triglycerides with the L-Type TG M kit (Wako Diagnostics, Wako Life Sciences Inc., Mountain View, CA).
9
Liver and Serum Lipid Analysis
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Liver lipids were extracted into chloroform-methanol (2:1) and solubilised in Triton X-100 as previously described (24) . Both liver lipids and serum lipids were determined enzymatically using Cholesterol Reagent (Pointe Scientific) and an L-Type TG M Kit (Wako Chemicals) for total cholesterol (TC) and TAG, respectively. Serum alanine aminotransferase (ALT) activity was measured using liquid ALT (SGPT) Reagent Set (Pointe Scientific). Serum glucose was measured using Liquid Glucose (Oxidase) Reagent Set (Pointe Scientific).
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