Cell count kit 8

We scrutinized the resilience of Hs 578T cellular configurations following siRNA exposure over a series of time intervals - namely, 0, 12, 24, 36, 48, and 72 hours. Simultaneously, an evaluation of the Hs 578T cells’ survival was performed using the Cell Count Kit-8 (Solarbio) method. This procedure entailed adding 10 μl of the CCK8 solution to each well, which was then subjected to a two-hour incubation at a stable environment of 37°C and a 5% CO2 atmosphere. Subsequent to the incubation, absorbance measurements at a 450 nm wavelength were precisely taken and recorded for further analysis.

Ginkgolide B (purity: 98% by HPLC) was supplied by the National Institutes for the Control of Pharmaceutical and Biological Products (Beijing, China). Pirfenidone (Cat number S2907) was obtained from Selleck (Shanghai, China). The drug of Yinxingneizhi Zhusheye (including Ginkgolide B) was obtained from Chengdu Baiyu Pharmaceutical Company. The drug of Pirfenidone capsules was purchased from Beijing Continent Pharmaceuticals Company. Tgfβ1 recombinant protein (Cat number H00007040-P01) was bought from Amyjet Scientific (Wuhan, China). The cell count kit-8 (Cat number CA1210) was bought from Solarbio Life Sciences (Shanghai, China). Rabbit monoclonal to Tgfβ1 (Cat number ab179695) was obtained from Abcam (CA, USA). Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb (Cat number #8828) was applied by Cell Signaling Technology. GAPDH monoclonal antibody (Cat number 60004-1-Ig) was bought from Proteintech (Rosemont, USA).