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Penicillin streptomycinwere

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Penicillin/streptomycin is a sterile solution containing the antibiotics penicillin and streptomycin. It is commonly used as a supplement in cell culture media to prevent bacterial contamination.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions) Acetic acid, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Sodium pyruvate, by Merck Group (1 mentions) Dulbecco s modified eagle s medium dmem, by Merck Group (1 mentions) Pei cellulose f plate, by Merck Group (1 mentions) Antibody against beta actin, by Santa Cruz Biotechnology (1 mentions) Optiphase hisafe 3, by PerkinElmer (1 mentions) Bj cells, by American Type Culture Collection (1 mentions) Escherichia coli atcc 25922, by American Type Culture Collection (1 mentions) Crl 2522, by American Type Culture Collection (1 mentions) Hyperbranched pei, by Merck Group (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Avantor (1 mentions) N acetyl cysteine (nac), by Merck Group (1 mentions) Staphylococcus aureus atcc 6538, by American Type Culture Collection (1 mentions) 1 6 hexanediol, by Thermo Fisher Scientific (1 mentions) Gwiz luc, by Aldevron (1 mentions) L proline, by Thermo Fisher Scientific (1 mentions)

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Penicillin (24825 citations) Streptomycinwere (3 citations)

14 protocols using penicillin streptomycinwere

1

Detailed TMPK Protein Immunoblotting Protocol

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Dulbecco’s modified Eagle’s
medium (DMEM) and sodium pyruvate were obtained from Sigma-Aldrich;
heat-inactivated fetal bovine serum (FBS) and penicillin–streptomycin
were obtained from Thermo Fisher; trypsin–EDTA was obtained
from the Swedish National Veterinary Institute; Bradford protein determination
solution was obtained from AppliChem; DEAE filter paper (DEAE filtermat)
and liquid scintillation fluid (Optiphase HiSafe 3) were purchased
from PerkinElmer; and a PEI cellulose F plate was obtained from Merck
group. The polyclonal antibody against human TMPK was produced by
using the C-terminal peptide sequence as antigen (GenScript Inc).9 (link) The antibody against beta-actin was from Santa
Cruz Biotechnology. Secondary fluorescence antibodies were from LI-COR
Biosciences.
Frisk J.H., Vanoevelen J.M., Bierau J., Pejler G., Eriksson S, & Wang L. (2021). Biochemical Characterizations of Human TMPK Mutations Identified in Patients with Severe Microcephaly: Single Amino Acid Substitutions Impair Dimerization and Abolish Their Catalytic Activity. ACS Omega, 6(49), 33943-33952.
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2

Functionalized Polymer Antimicrobial Coatings

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All reagents were used
as procured from the vendor unless otherwise mentioned. PVC (high
molecular weight), hyperbranched PEI (MW: 25,000), NAC, trioctyl trimellitate
(TOTM), tetrahydrofuran (THF), 1-ethyl-3-(3-dimethyl aminopropyl)
carbodiimide (EDC), N-hydroxysuccinimide (NHS), and
Tryptic soy and Luria–Bertani (LB) broth and agar were purchased
from Sigma-Aldrich (St. Louis MO, USA). SNAP was purchased from Pharma
block (Hatfield, PA, USA). Human fibroblasts [BJ cells, American type
culture collection (ATCC) CRL-2522], Staphylococcus
aureus (ATCC 6538), and Escherichia
coli (ATCC 25922) were procured from ATCC (Manassas
VA, USA). Dulbecco’s modified Eagle’s medium and penicillin–streptomycin
were from Thermo Fisher Scientific (Waltham MA, USA). FBS was obtained
from VWR (Atlanta, GA USA). All aqueous solutions were prepared in
Milli-Q water (18.2 MΩ) and purified using a Mettler Toledo
(Columbus, OH USA) apparatus. The drip flow bioreactor apparatus was
acquired from BioSurface Technologies Corporation (Model: DFR-110-6,
Bozeman, MT, USA).
Pandey R., Pinon V., Garren M., Maffe P., Mondal A., Brisbois E.J, & Handa H. (2024). N-Acetyl Cysteine-Decorated Nitric Oxide-Releasing Interface for Biomedical Applications. ACS Applied Materials & Interfaces, 16(19), 24248-24260.
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3

Synthesis and Characterization of Cationic Polymers

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1,1′-Carbonyldiimidazole
(CDI), bis(2-hydroxyethyl) disulfide (BHED), spermidine (SPM) trihydrochloride,
putrescine (PUT) dihydrochloride, and branched polyethylenimine (PEI,
25 kDa) were purchased from Sigma-Aldrich (St. Louis, MO). Dichloromethane
(DCM) (99.9%, extra dry, AcroSeal), tetrahydrofuran (THF, 99.85%,
extra dry, AcroSeal), 5-sulfosalicylic acid dihydrate (SSA), 1,7-diaminoheptane
(DAH), dansyl chloride (5-dimethylamino-1-naphthalenesulfonyl chloride,
98%), and 1,6-hexanediol were from Acros Organics (Fair Lawn, NJ). l-Proline was from Alfa Aesar (Ward Hill, MA). Spermine (SPM)
was from MP Biomedicals (Santa Ana, CA). BENSpm was synthesized by
following a previously described procedure.22 (link) Plasmid DNA containing a luciferase reporter gene (gWiz-Luc) was
from Aldevron (Fargo, ND), and human tumor necrosis factor (TNFα)
plasmid DNA was obtained from InvivoGen (San Diego, CA). Dulbecco’s
modified Eagle’s medium (DMEM), Eagle’s minimum essential
medium (EMEM), Dulbecco’s phosphate-buffered saline (PBS),
fetal bovine serum (FBS), l-glutamine, and penicillin–streptomycin
were from Thermo Scientific (Waltham, MA). RT-PCR primers were purchased
from Invitrogen (Carlsbad, CA). All other reagents and chemicals were
obtained from Fisher Scientific or VWR International unless otherwise
stated.
Zhu Y., Li J., Kanvinde S., Lin Z., Hazeldine S., Singh R.K, & Oupický D. (2014). Self-Immolative Polycations as Gene Delivery Vectors and Prodrugs Targeting Polyamine Metabolism in Cancer. Molecular Pharmaceutics, 12(2), 332-341.
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4

PEG-Based Scaffold for Bone Regeneration

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Poly(ethylene glycol) (PEG, Mn = 20,000 g/mol)
was purchased from Sigma-Aldrich Chemical Inc. (USA). Tannic acid
(TA, reagent) and phosphate-buffered saline (PBS, 0.01 M, pH 7.4)
were purchased from Alfa Aesar (United Kingdom). 1,4-Phenylenediboronic
acid (PDBA, C6H8B2O4, Mn
= 165.75 g/mol) and strontium ranelate (SrRan, C12H6N2O8SSr2·xH2O, Mn = 513.49 g/mol) were purchased from Tokyo Chemical
Industry (Japan). Sodium hydroxide (NaOH) was purchased from Merck
Inc. (Germany). Dulbecco′s modified Eagle medium (DMEM; Gibco),
fetal bovine serum (FBS; Gibco), and penicillin–streptomycin
were purchased from Thermo Fisher Scientific Inc. (USA). Minimal essential
medium alpha (MEM α; HyClone) was purchased from Cytiva (USA).
Sterile flat-bottom 24-well plates and transwell inserts (pore size
0.8 μm) were purchased from Corning Inc. (USA). All aqueous
solutions were prepared using distilled water. Titanium plate (grade
5, size 20 × 20 × 1 mm) was purchased from Prolog Titanium
Corporation (Thailand). Titanium powder (grade 5) was purchased from
TRUMPF Inc. (Germany).
Bubpamala T., Promoppatum P, & Pholpabu P. (2023). Drug-Releasing Tannic Acid-Mediated Adhesive PEG Hydrogel for Porous Titanium Implants. ACS Omega, 9(1), 887-895.
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5

Androstanolone and Flavonoid Treatments

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Androstanolone
(purity ≥98%) was purchased from Solarbio Science & Technology
Ltd. (Beijing, China). Quercetin (purity ≥98%), luteolin (purity
≥98%), kaempferol (purity ≥98%), baicalein (purity ≥98%),
calycosin (purity ≥98%), daidzein (purity ≥99%), genistein
(purity ≥98%), (+)-catechin (purity ≥98%), naringenin
(purity ≥98%), myricetin (purity ≥97%), and prunetin
(purity ≥99%) were all purchased from Mackin Regent (Shanghai,
China). Tectorigenin (purity ≥98%) was purchased from Shanghai
Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Dulbecco’s
modified Eagle’s medium (DMEM), fetal bovine serum (FBS), horse
serum, phosphate-buffered saline (PBS, pH 7.4), and penicillin-streptomycin
were provided by Gibco. A Cell Counting Kit-8 (CCK-8) was obtained
from DOJINDO (Japan).
Ji X., Li Q., Liu Z., Wu W., Zhang C., Sui H, & Chen M. (2024). Identification of Active Components for Sports Supplements: Machine Learning-Driven Classification and Cell-Based Validation. ACS Omega, 9(10), 11347-11355.
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6

Antiparasitic Compound Screening Protocol

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Amphotericin B (AmB), trivalent
antimony (SbIII), paromomycin (PMM), Triton X-100, 4′,6-diamidino-2-phenylindole
dilactate (DAPI), phorbol 12-myristate 13-acetate (PMA), resazurine,
and sodium dodecyl sulfate (SDS) were purchased from Sigma-Aldrich
(St. Louis, MO). Miltefosine (Mil) was purchased from Zentaris GmbH
(Frankfurt, Germany). l-Glutamine and penicillin/streptomycin
were obtained from Gibco. All chemicals were of the highest quality
available.
Perea-Martínez A., García-Hernández R., Manzano J.I, & Gamarro F. (2022). Transcriptomic Analysis in Human Macrophages Infected with Therapeutic Failure Clinical Isolates of Leishmania infantum. ACS Infectious Diseases, 8(4), 800-810.
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7

Chitosan Nanoparticles for Fibroblast Cells

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EA, chitosan (Cs) (low
molecular weight, purity ≥ 99%), acetic acid, Tween 80 (purity
≥ 99%), tripolyphosphate, ethanol, and cholesterol (purity
≥ 99.7%, MW = 386.65) were purchased
from Sigma-Aldrich Co. (Schnelldorf, Germany). Phosphate-buffered
saline (PBS) with a pH of 7.4 was purchased from Bio Shop Canada Inc.
Human fibroblast (HFB4) cells were obtained from Egypt’s Vaccines
and Sera (VACSERA) Tissue Culture Department. We purchased fetal bovine
serum (FBS) from Invitrogen Corp. (Carlsbad, CA). Hank’s buffer,
trypsin, ethylenediaminetetraacetic acid (EDTA), and penicillin–streptomycin
were obtained from Gibco (Grand Island, NY). BioVision, Inc. (Milpitas
CA) provided the Qiagen RNA extraction kit, BioRad SYBR green PCR
MMX kit, and TERT (Human) ELISA kit. Aktin Chemicals Inc. (Chengdu,
China) provided the punicalagin reference standard.
Abd-Elghany A.A, & Mohamad E.A. (2023). Chitosan-Coated Niosomes Loaded with Ellagic Acid Present Antiaging Activity in a Skin Cell Line. ACS Omega, 8(19), 16620-16629.
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8

Silane-Surfactant Nanoparticle Formation

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Triethoxysilane (TES), 1-octadecene, mesitylene,
and pluronic-F127 were purchased from Sigma Aldrich and used as received.
All other chemicals were acquired from Wako Pure Chemical Industries
Ltd. (Japan). Dulbecco’s modified Eagle media (DMEM) containing
10% fetal bovine serum (FBS) and 1% penicillin–streptomycin
were obtained from Gibco (Life Technologies, Carlsbad, California).
Ultrapure Milli-Q water (18 Ω) was produced using a Sartorius
(Arium 611 UV) water purification system and used in our experiments.
Nagarajan U., Chandra S., Yamazaki T., Shirahata N, & Winnik F.M. (2023). Analysis of Silicon Quantum Dots and Serum Proteins Interactions Using Asymmetrical Flow Field-Flow Fractionation. Langmuir, 39(22), 7557-7565.
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9

Compound Acquisition and Cell Culture Protocol

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Standard verbascoside,
hydroxytyrosol, and ferulic acid compounds were acquired from Extrasynthese
(Genay, France). Isoverbascoside and luteolin-7-diglucuronide were
purchased from PhytoLab (Vestenbergsgreuth, Germany). Caffeic acid
and homoprotocatechuic acid were obtained from Sigma-Aldrich (St.
Louis, MO, USA). Ethanol was provided by PanReac AppliChem GmbH (ITW
Reagents, Darmstadt, Germany). Methanol (HPLC-MS grade) and glacial
acetic acid were acquired from Fisher Scientific (Thermo Fisher Scientific,
Waltham, MA, USA) and Labkem (Labbox, Barcelona, Spain), respectively.
The water used in the HPLC-DAD-ESI-IT-MS analysis was purified using
a Milli-Q system from Millipore (Bedford, MA, USA). Dulbecco’s
modified Eagle’s medium (DMEM) and a mixture of penicillin/streptomycin
were acquired from Gibco (Thermo Fisher Scientific). Calf and fetal
bovine sera were obtained from Fisher Scientific. 3-Isobutyl-1-methylxanthine
(IBMX), dexamethasone, insulin, dimethyl sulfoxide (DMSO), and phosphate-buffered
saline (PBS) were provided by Sigma-Aldrich.
Olivares-Vicente M., Sánchez-Marzo N., Herranz-López M, & Micol V. (2024). Analysis of Lemon Verbena Polyphenol Metabolome and Its Correlation with Oxidative Stress under Glucotoxic Conditions in Adipocyte. Journal of Agricultural and Food Chemistry, 72(17), 9768-9781.
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10

Biochemical Assays for Liver Markers

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γ Oryzanol
(1479202), phosphatidylserine (PS; P7769), dimethyl
sulfoxide (DMSO; 67-68-5), tris (252859), Triton-X 100 (9036-19-5),
and phenylmethanesulfonyl fluoride (PMSF; 6-98-329) were obtained
from Sigma Company. RPMI-1640 medium, FBS, trypsin-EDTA, and penicillin–streptomycin
were purchased from Gibco, Rockville, MD. (ALT; BXC0215) and (AST;
BXC0205) assay kits were obtained from Biorexfars Co., Iran. Lipid
peroxidation (MDA) and catalase activity kits were from KiaZist Co.,
Iran. All additional chemicals and solvents were commercially available
and of analytical quality.
Jasim A.J., Albukhaty S., Sulaiman G.M., Al-Karagoly H., Jabir M.S., Abomughayedh A.M., Mohammed H.A, & Abomughaid M.M. (2024). Liposome Nanocarriers Based on γ Oryzanol: Preparation, Characterization, and In Vivo Assessment of Toxicity and Antioxidant Activity. ACS Omega, 9(3), 3554-3564.
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