Genomic DNA from phage F341 was isolated as described previously (Sørensen et al., 2021a (link)). The DNA concentration was estimated visually based on 0.7%–1% agarose gel electrophoresis as Campylobacter phages contain modified DNA (Crippen et al., 2019 (link)) that cannot be measured correctly by fluorometric quantification. Chromosomal DNA from C. jejuni NCTC12658 was isolated using Maxwell 16 Tissue DNA extraction kit (Promega) (Illumina sequencing) and the MagAttract HMW DNA kit (Qiagen) (SMRT sequencing, Pacific Biosciences) according to the manufacturer’s instructions. Chromosomal DNA from the LJ variants was isolated using Maxwell 16 Tissue DNA extraction kit (Promega) according to the manufacturer’s instructions.
Ostenfeld L.J., Sørensen A.N., Neve H., Vitt A., Klumpp J, & Sørensen M.C. (2024). A hybrid receptor binding protein enables phage F341 infection of Campylobacter by binding to flagella and lipooligosaccharides. Frontiers in Microbiology, 15, 1358909.
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