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Acethylthiocholine iodide

Manufactured by Merck Group
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Sourced in Italy

Acetylthiocholine iodide is a chemical compound used as a substrate in enzymatic assays to measure the activity of the enzyme acetylcholinesterase. It serves as a colorimetric or fluorometric indicator for the presence and quantification of this enzyme.

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Lab products found in correlation

Butyrylthiocholine iodide, by Merck Group (2 mentions) 4 hydroxybenzoic acid, by Merck Group (1 mentions) Bace 1 activity detection kit, by Merck Group (1 mentions) 5 5 dithiobis 2 nitrobenzoic acid dtnb, by Merck Group (1 mentions) Thioflavin t, by Merck Group (1 mentions) 3 4 dihydroxybenzoic acid, by Merck Group (1 mentions) Acetylcholinesterase, by Merck Group (1 mentions) Butyrylcholinesterase, by Merck Group (1 mentions) Nitric oxide, by Merck Group (1 mentions) Malondialdehyde, by Merck Group (1 mentions) Ferric chloride, by Merck Group (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Bradford reagent, by Merck Group (1 mentions)

2 protocols using acethylthiocholine iodide

1

Extraction and Characterization of Black Sesame Peptides

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BSP was prepared from black sesame seeds (Sesamum indicum L.) as previously described [46 (link)]. Pepsin from porcine gastric mucosa, pancreatin from porcine pancreas, porcine bile extract, 4-hydroxy-3-methoxybenzoic acid (VA), 4-hydroxybenzoic acid, 3,4-dihydroxybenzoic acid, KOH, NaOH, Na2S2O4, AChE from Electrophorus electricus (E.C.3.1.1.7, Type VI-S), acethylthiocholine iodide, 5,5′-dithiobis-2-nitrobenzoic acid (DTNB), BChE from equine serum (E.C.3.1.1.8), butyrylthiocholine iodide, thioflavin T, 1,1,1,3,3,3-hexafluoro-2-propanole (HFIP) and BACE1 activity detection kit were purchased from Sigma-Aldrich (Milan, Italy); synthetic Aβ 1-40 and 1-42 (human sequence) were from Bachem (Bubendorf, Switzerland).
Panzella L., Eidenberger T, & Napolitano A. (2018). Anti-Amyloid Aggregation Activity of Black Sesame Pigment: Toward a Novel Alzheimer’s Disease Preventive Agent. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(3), 676.
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2

Antioxidant and Cholinesterase Assays

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Malondialdehyde (MDA), ascorbic acid, methyl-2-phenylindole, nitric oxide, Ferric chloride, 2,6-di-tert-butyl 4-methylphenol, methanol, 5,5-dithiobis-2-nitrobenzoic acid, Bradford reagent, dimethyl sulphoxide (DMSO), acethylthiocholine iodide (ATCI), butyrylthiocholine iodide (BTCI), acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), and bovine serum albumin (BSA) were procured from Sigma-Aldrich. All other chemicals and reagents were of analytical grade.
Kpemissi M., Kantati Y.T., Veerapur V.P., Eklu-Gadegbeku K, & Hassan Z. (2022). Anti-cholinesterase, anti-inflammatory and antioxidant properties of Combretum micranthum G. Don: Potential implications in neurodegenerative disease. IBRO Neuroscience Reports, 14, 21-27.
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