Anti cd27 fitc

Manufactured by BD

Sourced in United States

Anti-CD27 FITC is a fluorescently labeled monoclonal antibody that binds to the CD27 cell surface antigen. CD27 is a member of the tumor necrosis factor receptor superfamily and is expressed on various immune cell types. The FITC (Fluorescein Isothiocyanate) label allows for the detection and analysis of CD27-expressing cells using flow cytometry and other fluorescence-based techniques.

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Lab products found in correlation

Anti cd8 percp, by BD (3 mentions) Anti cd127 pe, by BD (2 mentions) Anti cd4 percp, by BD (2 mentions) Anti cd4 fluoresceinisothiocyanate, by BD (1 mentions) Facsdiva software, by BD (1 mentions) Anti cd3 apc cy7, by BD (1 mentions) Anti cd25 pe cy7, by BD (1 mentions) Anti cd8 pe, by BD (1 mentions) 7 aad viability dye, by Thermo Fisher Scientific (1 mentions) Anti cd3 pe, by BD (1 mentions) Anti cd8 apc cy7, by BD (1 mentions) Foxp3 staining buffer, by Thermo Fisher Scientific (1 mentions) Anti cd3 efluor 450, by Thermo Fisher Scientific (1 mentions) Flojo software, by Tree Star (1 mentions) Anti cd28 pe, by BD (1 mentions) Facscalibur, by BD (1 mentions) Anti cd3 apc, by BD (1 mentions) Anti pd 1 pe, by BD (1 mentions) Anti cd56 pe, by BD (1 mentions) Cellquest software, by BD (1 mentions)

Spelling variants of this product

Anti-CD27 (32 citations) CD27-FITC (27 citations)

4 protocols using anti cd27 fitc

Comprehensive Immunophenotyping of T Cells

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Cells were stained with 7-AAD viability dye, anti-CD3 eFluor450, anti-CD4 PE-eFluor 647 (eBioscience), anti-CD4 electron coupled dye (Beckmann Coulter), anti-CD4 fluorescein isothiocyanate (FITC), anti-CD3 PE, anti-CD3 APC-Cy7, anti-CD8 PE, anti-CD8 APC-Cy7, anti-CD25 PECy7, anti-CD127 PE, and anti-CD27 FITC (BD), anti-CD45RA FITC, FOXP3 Alexa Fluor 647 (BioLegend) specific antibodies. FOXP3 intracellular staining was performed using FOXP3 staining buffers (eBioscience). Data were acquired using a fluorescence activated cell sorting (FACS)CantoII and analysed using FACSDiva software (BD) and Flojo software (Treestar).

Arroyo Hornero R., Betts G.J., Sawitzki B., Vogt K., Harden P.N, & Wood K.J. (2016). CD45RA Distinguishes CD4+CD25+CD127−/low TSDR Demethylated Regulatory T Cell Subpopulations With Differential Stability and Susceptibility to Tacrolimus-Mediated Inhibition of Suppression. Transplantation, 101(2), 302-309.

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Phenotypic Analysis of Activated T Cells

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OKT-CIK and R-CIK cells were measured on the 10th and 16th days after RetroNectin and OKT3 stimulating. The cells were harvested and washed in cold PBS and resuspended at 1 × 10⁶ in 100 μL cold PBS consisting of 5% serum. The cells were stained for anti-CD3-APC, anti-CD4-PerCP, anti-CD8-PerCP, anti-CD27-FITC, anti-CD28-PE, anti-CD56-PE, and anti-PD-1-PE purchased from BD Biosciences (San Jose, CA, USA) for 20 minutes on ice. FACS analysis was performed by using a FACSCalibur and CellQuest software (BD Biosciences, San Jose, CA, USA).

Han L., Shang Y.M., Song Y.P, & Gao Q.L. (2016). Biological Character of RetroNectin Activated Cytokine-Induced Killer Cells. Journal of Immunology Research, 2016, 5706814.

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Comprehensive Immune Cell Profiling

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Anti-CD3-FITC, anti-CD3-PerCP, anti-CD16/56-PE, anti-CD4-PerCP, anti-CD8-FITC, anti-CD8-PerCP, anti-CD38-PE, anti-DR-APC, anti-CD122-PE, anti-CD45RA-FITC, anti-CD45R0-PE, anti-CD62L-APC, anti-CD161-APC, anti-CD25-FITC, anti-CD127-PE, anti-CD27-FITC, anti-CD5-PerCP, anti-CD19-APC, CD107a-RE-Su5 (BD, Franklin Lakes, NJ, USA).

Toptygina A., Grebennikov D, & Bocharov G. (2023). Prediction of Specific Antibody- and Cell-Mediated Responses Using Baseline Immune Status Parameters of Individuals Received Measles–Mumps–Rubella Vaccine. Viruses, 15(2), 524.

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Multiparametric Phenotyping of T-Cell Subsets

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Cryopreserved PBMC and cells extracted from the gut biopsy specimens were rested overnight at 37°C before stimulation for 6 h with 40 ng/ml of phorbol myristate acetate (PMA) and 1 µM ionomycin in the presence of 1 µL/mL of brefeldin A (Sigma-Aldrich) to prevent cytokine release. After two washes with RPMI medium containing 10% heat-inactivated human serum (Gemini BioProduct), cells were fixed and permeabilized according to the eBioscience Fix/Perm protocol. The cells were stained with the following antibodies: anti-CD3 PE-Cy7 (Clone:SK7), anti-CD4 APC-Cy7 (Clone:SK3), anti-CD8 PacBlue (Clone:RPA-T8), anti-CD8 PerCP (Clone:SK1), anti-CD27 FITC (Clone:M-T271), anti-CD45RO APC (Clone:), anti-tumor necrosis factor (TNF) APC (Clone: 6401.1111), anti-Ki67 FITC (Clone:MOPC-21) from BD; anti-interferon γ (IFN-γ) PacBlue (Clone:4S.B3) and anti-IL-17 PE (Clone:ebio64Dec17) from eBioscience. Samples were acquired (approximately 200,000 events per sample) and analyzed as described above. In the gut mucosa, T-cells that were CD45RO⁺/CD27⁺ were identified as central memory (CM) and CD45RO⁺/CD27⁻ as effector memory (EM).

Sereti I., Estes J.D., Thompson W.L., Morcock D.R., Fischl M.A., Croughs T., Beq S., Lafaye de Micheaux S., Yao M.D., Ober A., Wilson E.M., Natarajan V., Imamichi H., Boulassel M.R., Lederman M.M, & Routy J.P. (2014). Decreases in Colonic and Systemic Inflammation in Chronic HIV Infection after IL-7 Administration. PLoS Pathogens, 10(1), e1003890.

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