Analytical grade acetonitrile (MeCN) was obtained from Carlo Erba. Patent blue V (HPLC purity ≥ 97%, CAS N°: 3536-49-0), Green S (HPLC purity ≥ 98%, CAS N°: 3087-16-9), Basic blue 3 (dye content 25%, CAS N°: 33203-82-6), methylene blue (HPLC purity ≥ 82%, CAS N°: 7220-79-3), and Acridine orange (dye content 75%, CAS N°: 494-38-2) were all purchased from Sigma-Aldrich. Brilliant green (1H NMR purity > 98%, CAS N°: 633-03-4) and Neutral red (1H NMR purity ≥ 92%, CAS N°: 553-24-2) were purified by crystallization from laboratory source. Azocarmine G (1H NMR purity ≥ 90%, CAS N°: 25641-18-3), Thymol (1H NMR purity ≥ 87%, CAS N°: 76-61-9) and Auramine O (1H NMR purity ≥ 80%, CAS N°: 2465-27-2) were used without purification. Proton Nuclear Magnetic Resonance analysis (1H NMR) was performed on a Varian 400 MHz spectrometer. Stock solutions (10 mM) were prepared by dissolving dyes into sterile water.
Patent blue 5
Manufactured by Merck Group
Sourced in United Kingdom, United States
Patent Blue V is a laboratory dye used for various applications in research and analysis. It is a synthetic dye with the chemical formula C₂₇H₃₁N₂NaO₆S. This dye is commonly used as a staining agent, tracer, and indicator in various scientific and medical procedures.
Automatically generated - may contain errors
Lab products found in correlation
400 mhz spectrometer, by Agilent Technologies (1 mentions)
Basic blue 3, by Merck Group (1 mentions)
Acridine orange, by Merck Group (1 mentions)
Methylene blue, by Merck Group (1 mentions)
Solvent green 3, by Merck Group (1 mentions)
Cmc na, by Merck Group (1 mentions)
Axio observer z1 epifluorescence microscope, by Zeiss (1 mentions)
Slide chemotaxis chamber, by Ibidi (1 mentions)
Assay buffer, by PerkinElmer (1 mentions)
Pherastar plus, by BMG Labtech (1 mentions)
Isopropyl myristate, by Merck Group (1 mentions)
Tetraglycol, by Merck Group (1 mentions)
Methanol, by Avantor (1 mentions)
Pvp k90, by Merck Group (1 mentions)
Millipore type 1 water, by Merck Group (1 mentions)
Pvp k60, by Merck Group (1 mentions)
Cremophor rh40, by Merck Group (1 mentions)
Croscarmellose sodium acdisol, by FMC Biopolymer (1 mentions)
Tween 20, by Merck Group (1 mentions)
Peg400, by Merck Group (1 mentions)
6 protocols using patent blue 5
1
Characterization of Dye Compounds by NMR Spectroscopy
2
Porcine Tissue-Derived PLG Microsphere Fabrication
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Porcine abdominal tissue was obtained (≤12 h post mortem) from a local abattoir from adult pigs that were not specifically slaughtered for this study.
PLG microspheres were prepared in-house using a coacervation method. Two suspending vehicles were selected with differing physicochemical attributes; a low viscosity (25 mPa.s at 20°C) non-aqueous vehicle, medium chain triglycerides (MCT), and a more viscous (122 mPa.s at 20°C) aqueous solution comprising 3% w/v carboxymethylcellulose sodium (CMC-Na) in phosphate-buffered saline containing 0.1% w/v polysorbate 20. MCT was obtained from Croda International (Goole, UK). CMC-Na, and colorimetric dyes (Patent Blue V and Solvent Green 3) were obtained from Sigma Aldrich (Gillingham, UK).
PLG microspheres were prepared in-house using a coacervation method. Two suspending vehicles were selected with differing physicochemical attributes; a low viscosity (25 mPa.s at 20°C) non-aqueous vehicle, medium chain triglycerides (MCT), and a more viscous (122 mPa.s at 20°C) aqueous solution comprising 3% w/v carboxymethylcellulose sodium (CMC-Na) in phosphate-buffered saline containing 0.1% w/v polysorbate 20. MCT was obtained from Croda International (Goole, UK). CMC-Na, and colorimetric dyes (Patent Blue V and Solvent Green 3) were obtained from Sigma Aldrich (Gillingham, UK).
3
Chemotaxis of Monocytes Expressing EGFP-Lifeact
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EGFP‐Lifeact ER‐Hoxb8 monocytes were seeded into a narrow channel of a fibronectin coated µ‐slide chemotaxis chamber (Ibidi) which is connected with two reservoirs. After 30 min, the chemotaxis chamber was filled with RPMI 1640 (bicarbonate) medium plus 10% FCS and 1% Pen/Strep. The reservoirs were filled with medium containing 0.003% Patent Blue V (Sigma–Aldrich) (blue dye) either with or without complement C5a (20 nm ). The observation area was imaged with an inverted Axio Observer.Z1 epifluorescence microscope (Zeiss) with 10×/0.25 objective. Images were acquired every minute for up to 6 h and analyzed with ImageJ (National Institutes of Health) using a manual tracking plugin and the chemotaxis and migration tool from Ibidi.
4
Quantification of Human PrP using CDI
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The CDI for human PrP was performed as described previously39 (link),79 (link), with following modifications: white Lumitrac 600 High Binding Plates (E&K Scientific, Santa Clara, CA) were coated with mAb 8H4 (epitope 175–185)80 (link) and aliquots of each sample containing 0.007% (v/v) of Patent Blue V (Sigma) were dilutedinto plate wells filled with 200 µl of Assay Buffer (Perkin Elmer, Waltham, MA); he captured PrP was detected by a europium-conjugated81 (link) anti-PrP mAb 3F4 (epitope 108–112)74 (link) and the time-resolved fluorescence (TRF) signals were measured by the multi-mode microplate reader PHERAstar Plus (BMG LabTech, Durham, NC).
5
Evaluation of UV Absorbing Compounds
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As a water-soluble absorber Patent Blue V (alternative name Acid Blue 3), the chemical name of which is 2-[(4-diethylaminophenyl)(4-diethylimino-2,5-cyclohexadien-1-ylidene)methyl]-4-hydroxy-1,5-benzene-disulfonate, from Sigma-Aldrich was employed.
Two oil-soluble UV absorbers were used, As continuous phase served bi-distilled water and hexanedioic acid dibutyl ester with the INCI name dibutyl adipate (DBA) p.a. from Aldrich.
Two particulate organic particulate UV absorbers were used as well. Those were 2,2′-methylene-bis-(6-(2H-benzotriazole-2-yl)-4-(1,1,3,3-tetramethylbutyl)-phenol with the INCI name methylene-bis-benzotriazolyl tetramethylbutylphenol (MBBT) and trade name Tinosorb® M (BASF), and 2,4,6-tris(biphenyl-3-yl)-1,3,5-triazine with INCI name trisbiphenyl-triazine (TBPT) and trade name Tinosorb® A2B (BASF). MBBT and TBPT, both, are commercially available as 50% (w) nano-particulate aqueous dispersions [6] .
Two oil-soluble UV absorbers were used, As continuous phase served bi-distilled water and hexanedioic acid dibutyl ester with the INCI name dibutyl adipate (DBA) p.a. from Aldrich.
Two particulate organic particulate UV absorbers were used as well. Those were 2,2′-methylene-bis-(6-(2H-benzotriazole-2-yl)-4-(1,1,3,3-tetramethylbutyl)-phenol with the INCI name methylene-bis-benzotriazolyl tetramethylbutylphenol (MBBT) and trade name Tinosorb® M (BASF), and 2,4,6-tris(biphenyl-3-yl)-1,3,5-triazine with INCI name trisbiphenyl-triazine (TBPT) and trade name Tinosorb® A2B (BASF). MBBT and TBPT, both, are commercially available as 50% (w) nano-particulate aqueous dispersions [6] .
6
Celecoxib Solid Dispersion Formulation
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Celecoxib (CXB), Eudragit S100, and hydroxypropyl methylcellulose acetate succinate LG (HPMCAS-LG) were supplied by Jansen Pharmaceutica (Beerse, Belgium). Capryol 90, Maisine CC, Peceol, Plurol oleique CC 497, and Labrasol were a gift from Gattefossé (Saint-Priest Cedex, France). Captex 200 P, Captex 355 EP/NF, and Capmul PG-8 NF were a gift from ABITEC Corporation (Columbus, OH, USA). Croscarmellose sodium (AcDiSol) was obtained from FMC BioPolymer (Philadelphia, PA, USA). Tween 20, isopropyl myristate, Cremophor RH40, PEG 400, tetraglycol, Patent Blue V, Povidone (PVP) K15, PVP K60, and PVP K90 were obtained from Sigma-Aldrich (St. Louis, MO, USA). Gelatine Licaps® size 0 capsules were gifted by Capsugel (Bornem, Belgium). Macrogolum 6000 (PEG 6000), PVP K30, hydroxypropyl methylcellulose (HPMC) 5 and 4000 cps, talc, and gelatine were obtained from BUFA (IJsselstein, The Netherlands). Tween 80, 37% fuming hydrochloric acid, sodium chloride (NaCl), sodium dodecyl sulfate (SDS), tert-butyl alcohol (TBA), sodium dihydrogen phosphate dihydrate, and sodium hydroxide (NaOH) were obtained from MERCK (Darmstadt, Germany). Acetone and ethanol 70% were purchased from BOOM B.V. (Meppel, The Netherlands) and methanol from VWR Chemicals (Fontenay-sous-Bois, France). Millipore type 1 water was used in all experiments except for the dissolution media where demineralized water was used.
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