Anti total p44 42 mapk

Manufactured by Cell Signaling Technology

Anti-total p44/42 MAPK is a primary antibody that detects the p44/42 mitogen-activated protein kinase (MAPK) protein, also known as ERK1/2. The antibody recognizes both the phosphorylated and unphosphorylated forms of the protein.

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P44/42 MAPK (90 citations) Anti-p44/42 MAPK (36 citations) Total p44/42 MAPK (12 citations) Total MAPK (9 citations) Total p44/42 (5 citations) Anti-MAPKs (4 citations) Anti-p44/42 MAPK (total ERK, (2 citations)

3 protocols using anti total p44 42 mapk

Investigating Intracellular Signaling Pathways

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Recombinant bovine FGF2, human FGF9, human TGFβ1, mouse noggin/Fc chimera, human BMP4, human BMP6, and anti-BMP antibody (MAB3552) were from R&D Systems (Minneapolis, MN). The following antibodies were all purchased from Cell Signaling Technology (Danvers, MA): anti–phospho-p44/42 MAPK E10 mouse monoclonal (#9106), anti–total p44/42 MAPK (#9102), anti–phospho-p38 (#9211), anti–phospho-MEK1/2 (#9154), anti–phospho Raf-1 (#9427), anti–phospho-FRS2-α(Tyr-196) (#3864), and anti–phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465) (#9511). Other antibodies used were as follows: for CP49, rabbit anti-mouse CP49 polyclonal serum (#899 or #900; both generous gifts of Paul FitzGerald, University of California, Davis, CA); for phospho-tyrosine, 4G10 (a kind gift from Brian Druker, Oregon Health and Science University, Portland, OR); for luciferase, #G745A from Promega (Madison, WI); for GFP, JL-8 from Clontech (Mountain View, CA); for phospho-Smad3, ab51451 from Abcam (Cambridge, MA); for total Smad 1/5, ab75273 from Abcam; for total Raf-1, sc-7267 from Santa Cruz Biotechnology (Santa Cruz, CA); for total p38, sc-535 from Santa Cruz; and for total MEK, M17030 from Transduction Labs (Lexington, KT). UO126 (used at 15 μM), PD173074 (100 nM), and dorsomorphin (5 μM) were from Calbiochem (La Jolla, CA). All other reagents, including TPA, were from Sigma-Aldrich (St. Louis, MO).

Boswell B.A, & Musil L.S. (2015). Synergistic interaction between the fibroblast growth factor and bone morphogenetic protein signaling pathways in lens cells. Molecular Biology of the Cell, 26(13), 2561-2572.

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Western Blot Antibody Detection Protocol

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Western immunoblots were run as described previously [15] (link). Primary antibodies and their sources were as follows. Anti-total p38 MAPK, anti-total p53, anti-total p44/42 MAPK, anti-phosphorylated p44/42 (pp44/42) MAPK, and anti-phosphorylated p53 (pp53) were from Cell Signaling Technology. Anti-phosphorylated p38 (pp38) MAPK was from Millipore. Anti-β-actin and the secondary antibodies horseradish peroxidase-conjugated anti-rabbit IgG and anti-rabbit IgG were from Sigma-Aldrich. Anti-goat IgG was from Santa Cruz Biotechnology.

Pei N., Jie F., Luo J., Wan R., Zhang Y., Chen X., Liang Z., Du H., Li A., Chen B., Zhang Y., Sumners C., Li J., Gu W, & Li H. (2014). Gene Expression Profiling Associated with Angiotensin II Type 2 Receptor-Induced Apoptosis in Human Prostate Cancer Cells. PLoS ONE, 9(3), e92253.

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Molecular Probes for Protein Analysis

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Anti-Mos (sc-86) was obtained from Santa Cruz Biotechnology, mouse anti-HA (#901501) from BioLegend, mouse anti-FLAG (F3165) from Sigma-Aldrich, rabbit anti-PABP1 (#4992), rabbit anti-phospho-p44/42 MAPK (#9101) and anti-total p44/42 MAPK (#9102) from Cell Signaling Technologies. For initial experiments determining ePABP and PABP1 expression, a polyclonal antibody directed against the C-terminal tail of ePABP was kindly provided by Joan Steitz (Voeltz et al., 2001 (link)). Rabbit polyclonal antibodies directed to Xenopus ePABP amino acids 449-462, were used for all other experiments (generated by Biosynthesis Inc., Lewisville, TX). The phospho-Cdk1 antibody was from Cell Signaling Technologies (#9111) and the total-Cdk1 antibody from Thermofisher Scientific (#33-1800).

Miedlich S.U., Taya M., Young M.R, & Hammes S.R. (2017). Paxillin and embryonic PolyAdenylation Binding Protein (ePABP) engage to regulate androgen-dependent Xenopus laevis oocyte maturation - a model of kinase-dependent regulation of protein expression. Molecular and cellular endocrinology, 448, 87-97.

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