Bks leprem2cd479 gpt

Manufactured by GemPharmatech

Sourced in China

The BKS-Leprem2Cd479/Gpt is a lab equipment product. It is used for conducting scientific experiments and analysis. The core function of this product is to facilitate the research and development process in a laboratory setting.

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Lab products found in correlation

B6.129s6 sjl fgf21tm1 2djm j, by Jackson ImmunoResearch (1 mentions) B6 cg speer6 ps1tg alb cre 21mgn j, by Jackson ImmunoResearch (1 mentions) Glucometer, by Bayer (1 mentions) Valsartan, by MedChemExpress (1 mentions) Telmisartan, by MedChemExpress (1 mentions) Gw9662, by MedChemExpress (1 mentions) Ins2 cre mice, by Jackson ImmunoResearch (1 mentions) Db m mice, by GemPharmatech (1 mentions) C57bl 6jgpt mice, by GemPharmatech (1 mentions)

7 protocols using bks leprem2cd479 gpt

Targeting FGF21 and GCGR in Diabetes

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All animal experimental procedures were conducted at Peking University Health Science Center and were approved by the Institutional Animal Care and Use Committee. Eight-week-old male db/db mice (BKS-Lepr^em2Cd479/Gpt [Strain no. T002407; GemPharmatech, Nanjing, China; https://www.gempharmatech.com/shop/detail/3913.html]) were used as a model of type 2 diabetes. Diabetes was induced in Fgf21^−/− (C57BL/6N-Fgf21^em1Cya [Cyagen Bioscience Company, Suzhou, China; https://www.cyagen.com/cn/zh-cn/sperm-bank-cn/S-KO-10895]) and Fgf21^Hep−/− mice (Alb-cre mice: B6.Cg-Speer6-ps1^{Tg(Alb-cre)21}Mgn/J [the Jackson Laboratory, Barr Harbor, ME, USA; https://www.jax.org/strain/003574]; Fgf21 Flox mice: B6.129S6(SJL)-Fgf21^tm1.2Djm/J [the Jackson Laboratory; https://www.jax.org/strain/022361]) [18 (link)] and male C57BL/6N mice (Vital River Animal Center, Beijing, China) by high-fat diet (HFD) + streptozotocin (STZ).
Mice were treated for 6 weeks via weekly i.p. injection of 5 mg/kg REMD 2.59 (a human antagonistic GCGR mAb; REMD Biotherapeutics, Camarillo, CA, USA) or human IgG (as control). Mice were treated with 1 mg/ml BrdU in their drinking water for 7 days before being killed. To antagonise FGF21 activity, db/db mice were given i.p. injections of FGF21 nAb (Antibody & Immunoassay Services, Hong Kong, China) or rabbit IgG (as control) daily for 3 weeks at a dose of 6 μg/day.

Cui X., Feng J., Wei T., Zhang L., Lang S., Yang K., Yang J., Liu J., Sterr M., Lickert H., Wei R, & Hong T. (2022). Pancreatic alpha cell glucagon–liver FGF21 axis regulates beta cell regeneration in a mouse model of type 2 diabetes. Diabetologia, 66(3), 535-550.

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Insulin Tolerance Test in BKS-db Mice

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BKS-db mouse (BKS-Leprem2Cd479/Gpt) was ordered from Gempharmatech Co., Ltd (Cat. No. T002407). Mice were fasted for overnight and injected intraperitoneally (i.p) with insulin 2 U kg⁻¹ body weight (Cat. No. 40112ES25, Yeasen Biotechnology (Shanghai) Co., Ltd.). Blood samples were taken before the glucose administration and then at 30, 60, 90 and 120 min after injection. Blood glucose levels were measured by a glucometer (Bayer HealthCare LLC).

Chen N., Lu B, & Fu Y. (2022). Autophagic Clearance of Lipid Droplets Alters Metabolic Phenotypes in a Genetic Obesity–Diabetes Mouse Model. Phenomics, 3(2), 119-129.

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Investigating Diabetic Heart Disease

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Six-eight-week-old male mice, including wild-type (WT) and BKS-Lepr^em2Cd479/Gpt (db/db) mice, were purchased from GemPharmatech Co. Ltd. All mice were maintained in the mouse barrier facilities of Dalian Medical University under 12 h light/dark cycles with free access to a normal diet and water. We measured blood glucose every week with a glucometer (PHI8080005) (Beijing, China) using the glucose oxidase method and glucose strips (DP0LM3F03A) (Beijing, China). At the age of 12 weeks, WT and db/db mice were intraperitoneally injected with 4-phenylbutyric acid (4-PBA) (HY-AO281) (25 mg/kg/day), which was purchased from MedChemExpress (Monmouth Junction, NJ, USA), or an equal amount of saline every other day until the hearts were extracted. WT and db/db mice were injected with rAAV9 expressing green fluorescent protein (rAAV9-GFP) or mitogen-activated protein kinase (MAPK) 10-specific short hairpin RNA (rAAV9-Sh-MAPK10) at the age of 6–8 weeks, and these viruses were produced by Hanbio (Shanghai, China) according to the manufacturer’s protocol. All animal experiments complied with the Guidelines of the Institutional Animal Care and Use Committee of Dalian Medical University, who approved all of the protocols.

Deng Y.W., Liu F., Li Z.T., Gao J.H., Zhao Y., Yang X.L, & Xia Y.L. (2022). Hyperglycemia promotes myocardial dysfunction via the ERS-MAPK10 signaling pathway in db/db mice. Laboratory Investigation; a Journal of Technical Methods and Pathology, 102(11), 1192-1202.

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Investigating Diabetes Medication Effects

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Adult male Wistar rats, weighing 240–260 g, were purchased from Beijing Weitong Lihua experimental animal center (Beijing, PR China). Five-week-old male diabetic db/db mice (BKS - Lepr^em2Cd479/Gpt, stock number T002407) were obtained from GemPharmatech Co., Ltd (Nanjing, China). Rats and mice were maintained in specific-pathogen-free surroundings, with a 12 h-light/dark cycles under controlled temperature (22 ± 2°C) and humidity (55–60%) conditions, and with free access to water and food. All animal care and experimental procedures conformed to the ethical guidelines for animal research at Shanxi Medical University and were approved by the Animal Care and Use Committee of Shanxi Medical University (Taiyuan, China). Telmisartan, valsartan, GW9662 were purchased from MedChemExpress (Shanghai, China), irbesartan was purchased from Aladdin (Shanghai, China).

Liu T., Cui L., Xue H., Yang X., Liu M., Zhi L., Yang H., Liu Z., Zhang M., Guo Q., He P., Liu Y, & Zhang Y. (2021). Telmisartan Potentiates Insulin Secretion via Ion Channels, Independent of the AT1 Receptor and PPARγ. Frontiers in Pharmacology, 12, 739637.

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Conditional circGlis3 Overexpression Mouse Model

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A conditional circGlis3 knock-in transgenic mouse model on a C57BL6/J background (circGlis3^fl/fl mice), in which circGlis3 and endogenous flanking sequence was targeted into the ROSA26 locus, was constructed by Cyagen Technology Company based on CRISPR/Cas9 technique. β-cell specific circGlis3 overexpression transgenic mice (Tg-circGlis3) were generated by crossing circGlis3^fl/fl mice with Ins2-Cre mice (Jackson Laboratory). Wild-type (WT) littermates were used as control. Genotyping of Tg-circGlis3 mice was performed by PCR using the primers listed in Table S3. Metabolic studies were performed on 12-week-old Tg-circGlis3 and control mice. Ten-week-old male db/db mice (BKS-Lepr^em2Cd479/Gpt) and age-matched male littermate db/m mice were purchased from the Gempharmatech Co., Ltd (Jiangsu, China) and used for islet isolation. All of the mice were housed in standard specific pathogen-free conditions with a 12 h light/dark cycle and had free access to water and food.

Xiong L., Gong Y., Liu H., Huang L., Zeng Z., Zheng X., Li W., Liang Z, & Kang L. (2023). circGlis3 promotes β-cell dysfunction by binding to heterogeneous nuclear ribonucleoprotein F and encoding Glis3-348aa protein. iScience, 27(1), 108680.

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Circadian Disruption Impact on Cognitive Function in db/db Mice

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The ve-week-old db/db mice (BKS-Lepr em2Cd479 /Gpt, strain number: T002407) were purchased from the GemPharmatech Co., Ltd (Nanjing, China). Mice were given 1 week to habituate to the facility and allowed ad libitum access to normal chow and water. For circadian disruption, one group (n=16) was kept on 6:18 light/dark cycle (6:18 LD cycle). The lights were switched at 7 am and off at 1 pm, which were regarded as zeitgeber time (ZT) 0 and ZT6 respectively. The control group (n=16) remained in a 12:12 light/dark cycle (12:12 LD cycle), with lights on at 7 am (ZT0) and off at 7 pm (ZT12). All animal experiments were approved by Animal Care and Use Committee of Tongji Hospital in accordance to the Public Health Service Policy on Human Care and Use of Laboratory animals.
At the age of 14 weeks, behavioral tasks were performed in db/db mice to assess their cognitive ability. Then they were euthanized every six hours in one day (ZT0, ZT6, ZT12 and ZT18) to evaluate the effect of light on diurnal rhythmicity. Their brains were removed and sagittally bisected. The right hemisphere was xed in 4% paraformaldehyde for immunohistochemical analysis; the hippocampus was separated from the left hemisphere and frozen at -80 °C for later western blotting.

Huang J., Peng X., Fan R., Dong K., Shi X., Zhang S., Yu X., & Yang Y. (2021). Disruption of circadian clocks promote progression of Alzheimer's disease in diabetic mice.

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Diabetes Induction in Mouse Models

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Twelve-week-old male db/db mice (BKS-Leprem2Cd479/Gpt, GemPharmatech, Nanjing, China) were used as a T2DM mouse model. Male C57BL/6JGpt mice (GemPharmatech) and nuclear factor erythroid 2-related factor 2 knockout (Nrf2-KO) mice (kindly provided by Prof. Xuebo Pan at Wenzhou Medical University) aged 8–12 weeks were used to establish T1DM model by streptozotocin (STZ, Sigma, St. Louis, MO, USA) injection (50 mg/kg body weight, one dose a day for 5 days). Those mice with a fasting blood glucose higher than 13.8 mM at the 7th day after the last STZ injection were considered as T1DM mice. Mice were maintained at 22 ± 2 °C with a 12 h light/dark cycle. All animal experimental procedures were carried out in accordance with the policies of Institutional Animal Care and the Use Committee of Wenzhou Medical University.

Yan X., Su Y., Fan X., Chen H., Lu Z., Liu Z., Li Y., Yi M., Zhang G., Gu C., Wang K., Wu J., Sun D., Zhang Y., Zhang C., Dai X, & Zheng C. (2022). Liraglutide Improves the Angiogenic Capability of EPC and Promotes Ischemic Angiogenesis in Mice under Diabetic Conditions through an Nrf2-Dependent Mechanism. Cells, 11(23), 3821.

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