Hematoxylin eosin he kit

At 12 hours, 3 days, and 14 days after BBB score, the rats were anesthetized and per-fused transcardially with 200 mL of phosphate-buffered saline (PBS) (0.1 mol/L, pH 7.4), followed by 400 mL of PBS (pH 7.4) with 4% paraformaldehyde (PFA). The injury epicenter (about 3-cm-long piece) was excised from the spinal cord and post-fixed in 4% PFA overnight. Then fixed tissues were embedded in paraffin and serially sectioned into 4-μm thick coronal slices and stained with Hematoxylin-Eosin/HE kit (Solarbio Science & Technology, Beijing, China) following standard protocols.

The detached left kidney were rapidly fixed in 10% paraformaldehyde solution for 24 hours, then tissues was dehydrated in gradient alcohol, embedded in paraffin, sectioned (4 μm), stained with Hematoxylin-Eosin (HE) kit (Solarbio, Beijing, China, G1120) and Masson’s Trichrome (Masson) kit (Solarbio, Beijing, China, G1340). Sections were placed under an optical microscope (Olympus Corporation, Japan) at 400x magnification for imaging and photographed. Renal injury including tubular and glomerular deformation and tubular epithelial cell detachment and assessed by semi-quantitative analysis as described by Choi et al.26 (link) With the following criteria: 0: no abnormality; 1: impairment less than 10%; 2: impairment less than 25%; 3: impairment less than 50%; 4: impairment less than 75%; 5: impairment greater than 75%. Semi-quantitative analysis of fibrotic area was assessed by Masson staining, ie calculating the percentage of blue staining over the entire view.27 (link) Both semi-quantitative assessments were performed on 10 randomly selected non-overlapping areas under the optical microscope.