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Transam nrf2 assay kit

Manufactured by Active Motif
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The TransAM NRF2 assay Kit is a DNA-binding ELISA-based assay that measures the activation and DNA-binding activity of the transcription factor Nrf2 (Nuclear factor erythroid 2-related factor 2) in nuclear extracts. The kit provides a simple and high-throughput method for quantifying active Nrf2 levels in a variety of cell and tissue samples.

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Lab products found in correlation

Microplate reader, by Thermo Fisher Scientific (2 mentions) Microplate reader at 450 nm, by Molecular Devices (1 mentions)

Close spelling variants of this product

TransAm Nrf2 assay TransAM Nrf2 kit TransAM Nrf2 TransAM kit

4 protocols using transam nrf2 assay kit

1

NRF2 DNA-Binding Assay Protocol

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NRF2 DNA-binding assay was performed by TransAM NRF2 assay Kit (Active Motif, Carlsbad, CA) according to the manufacturer's manual.
Kang H.J., Yi Y.W., Hong Y.B., Kim H.J., Jang Y.J., Seong Y.S, & Bae I. (2014). HER2 confers drug resistance of human breast cancer cells through activation of NRF2 by direct interaction. Scientific Reports, 4, 7201.
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2

Measuring Nrf2 DNA Binding via TransAM Assay

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The DNA binding of Nrf2 was measured using a specific TransAM® Nrf2 assay kit (Active Motif), according to the manufacturer’s instructions. Briefly, the nuclear extracts were incubated in the oligonucleotide-coated wells. Subsequently, the wells were washed twice with PBS and incubated with antibody against Nrf2 at 37°C. The addition of secondary antibody conjugated to HRP provided a colorimetric readout. The absorbance of each well was measured using a microplate reader at 450 nm (Molecular Devices).
JEONG S.O., SON Y., LEE J.H., CHEONG Y.K., PARK S.H., CHUNG H.T, & PAE H.O. (2015). Resveratrol analog piceatannol restores the palmitic acid-induced impairment of insulin signaling and production of endothelial nitric oxide via activation of anti-inflammatory and antioxidative heme oxygenase-1 in human endothelial cells. Molecular Medicine Reports, 12(1), 937-944.
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3

Nrf2 Activity Assay Protocol

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Nrf2 activity was measured by using a TransAM Nrf2 assay kit (Active Motif, Carlsbad, CA) as previously described 28 (link). The cell nuclear extracts (5 μg) were added to a 96-well plate that had been precoated with an oligonucleotide with a binding site for Nrf2. The absorbance was detected at 450 nm by a microplate reader.
Zhang M., Xu Y., Qiu Z, & Jiang L. (2019). Sulforaphane Attenuates Angiotensin II-Induced Vascular Smooth Muscle Cell Migration via Suppression of NOX4/ROS/Nrf2 Signaling. International Journal of Biological Sciences, 15(1), 148-157.
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4

Nrf2 Activity Quantification Assay

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Nrf2 activity was detected using a TransAM NRF2 assay Kit (Active Motif, Carlsbad, CA) according to the protocol. First, 5 μg aliquots of the nuclear extracts of the HUVECs were incubated in a 96-well plate that had been precoated with an oligonucleotide containing a binding site for Nrf2. The absorbance was detected at 450 nm using a microplate reader (Thermo Scientific, CA, USA).
Zhang M., Pan H., Xu Y., Wang X., Qiu Z, & Jiang L. (2017). Allicin Decreases Lipopolysaccharide-Induced Oxidative Stress and Inflammation in Human Umbilical Vein Endothelial Cells through Suppression of Mitochondrial Dysfunction and Activation of Nrf2. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 41(6).
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