Ly6g antibody

Manufactured by Abcam

The Ly6G antibody is a laboratory research tool used to detect the presence and localization of the Ly6G protein. Ly6G is a cell surface antigen that is expressed on granulocytes, particularly neutrophils. The antibody can be used in various immunological techniques, such as flow cytometry, immunohistochemistry, and western blotting, to study the biology and function of these cell types.

Automatically generated - may contain errors

Lab products found in correlation

Qscript complementary dna cdna synthesis kit, by Quanta Biosciences (3 mentions) Neutrophil elastase, by R&D Systems (2 mentions) Mouse lamina propria dissociation kit, by Miltenyi Biotec (2 mentions) Myeloperoxidase, by R&D Systems (2 mentions) Kanamycin, by Merck Group (2 mentions) Nalidixic acid, by Merck Group (2 mentions) Duoset enzyme linked immunosorbent assay, by R&D Systems (2 mentions) Interleukin il 22, by R&D Systems (2 mentions) Sybr green mix, by Quanta Biosciences (2 mentions) Alcian blue ph 2.5 stain kit, by Vector Laboratories (2 mentions) Cl amidine hydrochloride, by Cayman Chemical (2 mentions) Il 17a, by R&D Systems (2 mentions) Rpmi 1640, by Merck Group (2 mentions) Paraformaldehyde (pfa), by Merck Group (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Luria bertani broth, by Merck Group (2 mentions) Histopaque 1077 and 1119, by Merck Group (2 mentions) Serum amyloid a, by R&D Systems (2 mentions) Guaiacol 2 methoxyphenol, by Thermo Fisher Scientific (2 mentions) α hu lcn2 sybr green mix, by Quanta Biosciences (2 mentions)

7 protocols using ly6g antibody

Extraction and Quantification of Murine Immune Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Histopaque-1077 and −1119, RPMI 1640, Luria-Bertani (LB) broth, bovine serum albumin (BSA), paraformaldehyde, nalidixic acid and kanamycin were procured from Sigma (St. Louis, MO). DNase I (dornase alfa, Pulmozyme®) was kindly gifted from Genentech. Duoset enzyme-linked immunosorbent assay (ELISA) kits for mouse Lipocalin2 (Lcn2), keratinocyte-derived chemokine (KC), serum amyloid A (SAA), neutrophil elastase (NE), myeloperoxidase (MPO), interleukin (IL)-22 and IL-17A were obtained from R&D Systems (Minneapolis, MN). SYBR Green mix and the qScript complementary DNA (cDNA) synthesis kit were procured from Quanta BioSciences (Gaithersburg, MD). Ly6G antibody was purchased from Abcam (Cambridge, MA). All other fine chemicals used in the present study were reagent grade and procured from Sigma. Alcian Blue (pH 2.5) Stain Kit was obtained from Vector Laboratories (Burlingame, CA). Mouse Lamina Propria Dissociation Kit was purchased from Miltenyi Biotec (Auburn, CA). Cl-amidine (hydrochloride) was obtained from Cayman chemicals (Ann Arbor, MI).

Saha P., Yeoh B.S., Xiao X., Golonka R.M., Singh V., Wang Y, & Vijay-Kumar M. (2019). PAD4-dependent NETs generation are indispensable for intestinal clearance of Citrobacter rodentium. Mucosal immunology, 12(3), 761-771.

+ Open protocol

+ Expand

Detailed Reagent Specifications for Colitis Study

Check if the same lab product or an alternative is used in the 5 most similar protocols

Reagent grade Dextran Sulfate Sodium salt (DSS, reagent grade, M.W 36–50kDa, Ref = 160110) was purchased from MP Biomedicals (Solon, OH). Duoset ELISA kits for mouse and human Lcn2 and keratinocyte-derived chemokine (KC) were obtained from R&D Systems (Minneapolis, MN). α-Hu-Lcn2 SYBR® Green mix and qScript cDNA synthesis kit was procured from Quanta Biosciences (Gaithersburg, MD). Guaiacol (2-methoxyphenol) was obtained from Alfa Aesar (Ward Hill, MA). Myeloperoxidase (MPO) was procured from Sigma (St. Louis, MO). Ly6G antibody was purchased from Abcam (Cambridge, MA). Rat anti-mouse IL-10 receptor (IgG₁) monoclonal antibody (αIL-10R) and rat anti-mouse PD-1 monoclonal antibody (IgG1 isotype control) were procured from BioXcell (West Lebanon, NH). Anti-mouse CD45-Alexafluor 594 and CD326 monoclonal antibodies were procured from BioLegend (San Diego, CA) RegIIIγ and Ang4 antibodies are kind gift from Dr. Lora Hooper, UT Southwestern Medical Center, TX. All other fine chemicals used in present study were reagent grade and procured from Sigma.

Singh V., Yeoh B.S., Chassaing B., Zhang B., Saha P., Xiao X., Awasthi D., Shashidharamurthy R., Dikshit M., Gewirtz A, & Vijay-Kumar M. (2016). Microbiota-Inducible Innate Immune Siderophore Binding Protein Lipocalin 2 Is Critical for Intestinal Homeostasis. Cellular and Molecular Gastroenterology and Hepatology, 2(4), 482-498.e6.

+ Open protocol

+ Expand

Extraction and Quantification of Murine Immune Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Antibody Use in Neurological Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following primary antibodies were used in this study: goat anti-human SCARB2 antibody (R&D Systems; catalog no. AF1966), mouse anti-EV71 antibody (GeneTex, Inc.; catalog no. GTX41306). Rabbit anti-IBA-1 antibody (catalog no. GB13105-1), NeuN antibody (catalog no. GB11138), and caspase-3 antibody (catalog no. GB11138) were from Servicebio Biotech. Rabbit anti-CD3 antibody (catalog no. ab133357), GFAP antibody (catalog no. ab7260), Ly6G antibody (catalog no. ab25377), rat anti-CD45 antibody (catalog no. ab25386), and mouse anti-CD45 antibody (catalog no. ab33923) were from Abcam, Inc.

Jin Y., Sun T., Zhou G., Li D., Chen S., Zhang W., Li X., Zhang R., Yang H, & Duan G. (2021). Pathogenesis Study of Enterovirus 71 Using a Novel Human SCARB2 Knock-In Mouse Model. mSphere, 6(2), e01048-20.

+ Open protocol

+ Expand

Dextran Sulfate Sodium-Induced Colitis Model

Check if the same lab product or an alternative is used in the 5 most similar protocols

Reagent grade dextran sulfate sodium salt (DSS) (reagent grade; molecular weight, 36–50 kilodaltons; catalog number 160110) was purchased from MP Biomedicals (Solon, OH). Duoset enzyme-linked immunosorbent assay (ELISA) kits for mouse and human Lcn2 and keratinocyte-derived chemokine were obtained from R&D Systems (Minneapolis, MN). α-Hu-Lcn2 SYBR Green mix and the qScript complementary DNA (cDNA) synthesis kit were procured from Quanta BioSciences (Gaithersburg, MD). Guaiacol (2-methoxyphenol) was obtained from Alfa Aesar (Ward Hill, MA). MPO was procured from Sigma (St. Louis, MO). Ly6G antibody was purchased from Abcam (Cambridge, MA). Rat anti-mouse IL10 receptor (IgG₁) monoclonal antibody (αIL10R) and isotype control antibody (rat anti-mouse IgG1) were procured from BioXcell (West Lebanon, NH). Anti-mouse cluster of differentiation 45 (CD45)–Alexa Fluor 594 and CD326 monoclonal antibodies were procured from BioLegend (San Diego, CA). RegIIIγ and Ang4 antibodies were a kind gift from Dr Lora Hooper (UT Southwestern Medical Center, Dallas, TX). All other fine chemicals used in the present study were reagent grade and procured from Sigma.

+ Open protocol

+ Expand

Mucosal Inflammatory Response Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The middle of the colon was fixed in 4% formaldehyde. Histological sections (4 μm) were stained with HE for morphometric examination. The score was according to the degree of inflammatory infiltration (0–5), crypt injury (0–4), ulcer (0–3), and the absence of edema (0 or 1), as described by Stillie et al. (13 (link)).
The slides were deparaffinized and then incubated with F4/80 antibody (Abcam, 1:100), Ly6G antibody (Abcam, 1:100), CD4 antibody (Servicebi, 1:100), Muc2 antibody (Proteintech, 1:100), Claudin-1 antibody (Proteintech, 1:100) and ZO-1 antibody (Proteintech, 1:100) in blocking buffer overnight at 4°C overnight, followed by incubation with Alexa Fluor 594-labeled second antibody (Proteintech) for 1 h. The sections were then stained with DAPI for nuclear counterstaining. The images were observed by fluorescence microscopy (Olympus IX71, Tokyo, Japan).
The slides were deparaffinized, stained with Phospho-NF-κB p65 (Servicebio, 1:100) and Phospho-STAT3 antibody (Abcam, 1:100) overnight at 4°C, incubated with a rabbit HRP polyclonal antibody for 1 h at room temperature, and finally visualized with DAB (VECTOR).

Yang H., Cai R., Kong Z., Chen Y., Cheng C., Qi S, & Gu B. (2020). Teasaponin Ameliorates Murine Colitis by Regulating Gut Microbiota and Suppressing the Immune System Response. Frontiers in Medicine, 7, 584369.

+ Open protocol

+ Expand

Histological Analysis of Limb Ischemia

Check if the same lab product or an alternative is used in the 5 most similar protocols

Histological analysis was performed on both the ischemic and non-ischemic limbs at indicated days post hind limb ischemia surgery. Mice were euthanized and the tissue was perfused with saline followed by 10% buffered formalin for fixation. The bone was then demineralized in a formic acid-based solution (Cal-Ex II; Fisher Scientific) for 48 h before processing and paraffin embedding. 5- μm-thick sections were prepared for staining. Enzyme treatment was performed in 2 μg/ml proteinase K (Biolabs; Cat # P8107S) prior to incubation with primary antibodies. Sections were stained with Ly6G antibody (abcam Cat # ab25377), smooth muscle α-actin antibody (Sigma Cat # A2547) followed by incubation with Streptavadin QDot 655 (Invitrogen; Cat # Q10121MP).

Okwan-Duodu D., Hansen L., Joseph G., Lyle A.N., Weiss D., Archer D.R, & Taylor W.R. (2018). Impaired Collateral Vessel Formation in Sickle Cell Disease. Arteriosclerosis, thrombosis, and vascular biology, 38(5), 1125-1133.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!