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Mir 1297 mimics inhibitor

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MiR-1297 mimics/inhibitor is a laboratory reagent used to modulate the expression of the miR-1297 microRNA. It can be used to either increase or decrease the cellular levels of miR-1297 for research purposes. No further details on intended use can be provided.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Pcdna3.1 empty vector, by Thermo Fisher Scientific (1 mentions) Nc mimics inhibitor, by GenePharma (1 mentions) Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions)

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MiR-mimics (3 citations)

2 protocols using mir 1297 mimics inhibitor

1

Silencing DUXAP8 and Overexpressing RCN2 in Cancer Cell Lines

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Silencing of DUXAP8 and overexpression of RCN2 in caSki and HeLa cell samples were severally achieved by transfection with the duplex shRNAs (Genepharma, Shanghai, China) against DUXAP8 and pcDNA3.1/RCN2. The nonspecific shRNAs and empty pcDNA3.1 vector (Invitrogen) served as the negative control (NC). Besides, miR-1297 mimics/inhibitor and NC mimics/inhibitor were also procured from Genepharma. Cell transfection was performed for 48 h with Lipofectamine 2000 (Invitrogen).
Gu J., Liu Y., Qi T., Qian W., Hu D, & Feng W. (2021). Long non-coding RNA DUXAP8 elevates RCN2 expression and facilitates cell malignant behaviors and angiogenesis in cervical cancer via sponging miR-1297. Diagnostic Pathology, 16, 105.
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2

Targeted ADM Knockdown and miR-1297 Modulation

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Short hairpin RNA targeting ADM (sh1-ADM or sh2-ADM) was used to achieve the knockdown of ADM (GenePharma, Shanghai, China). miR-1297 expression was intervened through the transfection of miR-1297 mimics/inhibitor (GenePharma). All the transfection was performed using Lipofectamine 3000 reagent (Invitrogen). Cells were seeded in 6/96-well plates. After transfection, cells were subjected to 24/48-h incubation for subsequent analysis.
He Z., Cheng M., Hu J., Liu L., Liu P., Chen L., Cao D, & Tang J. (2022). miR-1297 sensitizes glioma cells to temozolomide (TMZ) treatment through targeting adrenomedullin (ADM). Journal of Translational Medicine, 20, 443.
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