Ammonium 7 fluoro 2 1 3 benzoxadiazole 4 sulfonate sbd f
Manufactured by Dojindo Laboratories
Sourced in Japan
Ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F) is a fluorescent labeling reagent used for the detection and quantification of amino compounds. It exhibits strong fluorescence when reacted with primary amines.
Automatically generated - may contain errors
Lab products found in correlation
Milli q system, by Merck Group (3 mentions)
Phosphate buffered saline (pbs), by Takara Bio (2 mentions)
L gsh, by Merck Group (2 mentions)
Cysgly, by Merck Group (2 mentions)
L cys, by Merck Group (2 mentions)
Dl hcy, by Merck Group (2 mentions)
γ glucys, by Merck Group (1 mentions)
Trichloroacetic acid, by Fujifilm (1 mentions)
N acetyl l cysteine, by Fujifilm (1 mentions)
Trichloroacetic acid tca, by Fujifilm (1 mentions)
Hplc grade meoh, by Merck Group (1 mentions)
Citric acid, by Fujifilm (1 mentions)
3 protocols using ammonium 7 fluoro 2 1 3 benzoxadiazole 4 sulfonate sbd f
1
Quantification of Thiol Compounds
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l -Cys, D ,l -Hcy, l -GSH, CysGly, and γGluCys were purchased from Sigma-Aldrich (St. Louis, MO, USA). N-Acetyl-l-Cysteine (NAC), tiopronin (N-(2-mercaptopropionyl)glycine, MPG), and trichloroacetic acid (TCA) were obtained from Wako (Osaka, Japan). Ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F) was purchased from Dojindo (Kumamoto, Japan). TCEP was from Tokyo Chemical Industry (Tokyo, Japan). Phosphate buffered saline (PBS) was purchased from Takara Bio (Shiga, Japan). HPLC-grade acetonitrile was used. Water was purified using a Milli-Q system (Millipore, Bedford, MA, USA). All other chemicals were of analytical grade.
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2
Quantification of Thiol-Containing Compounds
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L -Cys, D ,L -Hcy, L -GSH, and CysGly were purchased from Sigma-Aldrich (St. Louis, MO, USA). Trichloroacetic acid (TCA) was obtained from Wako Pure Chemical (Osaka, Japan). Ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F) was purchased from Dojindo (Kumamoto, Japan). TCEP was purchased from the Tokyo Chemical Industry (Tokyo, Japan). Phosphate buffered saline (PBS) was purchased from Takara Bio (Shiga, Japan). HPLC-grade acetonitrile was used. Water was purified using a Milli-Q system (Millipore, Bedford, MA, USA). All other chemicals were of analytical grade.
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3
Thiol Compound Quantification Protocol
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Cysteine (Cys), homocysteine (Hcy), cysteinylglycine (CysGly), and γ-glutamylcysteine (γGC) were from Sigma-Aldrich (St. Louis, MO, USA). Glutathione (GSH), N-acetylcysteine (NAC), sodium acetate, citric acid, potassium dihydrogen citrate, and tris(2-carboxyethyl) phosphine (TCEP) were obtained from FUJIFILM Wako Pure Chemical (Osaka, Japan). Ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F) was obtained from Dojindo Laboratories (Kumamoto, Japan). MeOH (HPLC grade) was from Merck KGaA (Darmstadt, Germany). A Milli-Q system (Merck) was used for water purification.
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