Log In Sign up
The largest database of trusted experimental protocols

6550 ifunnel quadrupole time of flight q tof mass spectrometer

Manufactured by Agilent Technologies
Visit Supplier
Sourced in United States

The 6550 iFunnel Quadrupole Time-of-Flight (Q-TOF) mass spectrometer is a highly sensitive and accurate analytical instrument designed for the detection and identification of a wide range of compounds. It combines a quadrupole mass analyzer with a time-of-flight mass analyzer to provide high-resolution mass spectra.

Automatically generated - may contain errors

Lab products found in correlation

Mass profiler professional software, by Agilent Technologies (2 mentions) Hplc chip cube interface, by Agilent Technologies (1 mentions)

Spelling variants of this product

6550 iFunnel Q-TOF (31 citations) 6550 Q-TOF (30 citations) 6550 Q-TOF mass spectrometer (27 citations) 6550 iFunnel Q-TOF mass spectrometer (23 citations) Spectrometers (10 citations) 6550 quadrupole time-of-flight mass spectrometer (5 citations) 6550 quadrupole/time-of-flight (Q-TOF) mass spectrometer (2 citations) 6550 iFunnel Quadrupole Time of Flight mass spectrometer (2 citations) Agilent 6550 iFunnel quadrupole-time-of-flight (Q-TOF) mass spectrometer (2 citations)

3 protocols using 6550 ifunnel quadrupole time of flight q tof mass spectrometer

1

N-Glycan Profiling and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
An Agilent 1260 Infinity HPLC Chip LC system was coupled to an Agilent 6550 iFunnel Quadrupole Time-of-Flight (Q-TOF) mass spectrometer (MS) for N-glycan profiling or coupled to an Agilent 6490 iFunnel Triple Quadrupole (QQQ) MS for N-glycan quantification. The Agilent 1260 Infinity HPLC Chip system was equipped with a HiP micro ALS sampler with a 40 µl sample loop, a nanoflow pump, a capillary pump, a HPLC Chip Cube Interface, a thermostat and a µ-degasser. A 25 µm ID PEEK capillary was used for sample transfer to prevent the dissolution of fused silica by the high-pH elution buffer.
Wang J.R., Gao W.N., Grimm R., Jiang S., Liang Y., Ye H., Li Z.G., Yau L.F., Huang H., Liu J., Jiang M., Meng Q., Tong T.T., Huang H.H., Lee S., Zeng X., Liu L, & Jiang Z.H. (2017). A method to identify trace sulfated IgG N-glycans as biomarkers for rheumatoid arthritis. Nature Communications, 8, 631.
+ Open protocol
+ Expand
2

Untargeted Metabolomics Analysis of CC-CM Treated C2C12 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three biological replicates of control and each CC-CM treated C2C12
cells were prepared for untargeted metabolomics analyses as follows: cells were
grown to 60% confluency and then treated with CC-CM for 48 hours, at which time
plates were washed with PBS and cells were scraped and pelleted, followed by
storage at −80° C. Samples were submitted to the Metabolomics Core
at Mayo Clinic (Rochester, MN, USA) for metabolomics profiling by liquid
chromatography-mass spectrometry (LC/MS) using 6550 iFunnel Quadrupole Time of
Flight (Q-TOF) mass spectrometer (Agilent). Metabolites were then identified
using METLIN database with Mass Profiler Professional software (Agilent).
Annotated metabolites with a p value less than 0.05 and a fold change greater
than 2 were further analyzed using MetaboAnalyst network explorer44 (link) (metabolite-metabolite
interactions) and enrichment analysis (pathway associated metabolite sets)
tools. KEGG and HMDB identifiers were used in MetaboAnalyst.
Arneson-Wissink P.C, & Doles J.D. (2021). Disrupted NOS2 metabolism drives myoblast response to wasting-associated cytokines. Experimental cell research, 407(1), 112779.
+ Open protocol
+ Expand
3

Untargeted Metabolomics Analysis of C2C12 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three biological replicates of control and DM-succinate (8 mM) treated
C2C12 cells were prepared for untargeted metabolomics analyses as follows: cells
were grown to 60% confluency and then treated with 8 mM succinate for 48 hours,
at which time plates were washed with PBS and cells were scraped and pelleted,
followed by storage at −80° C. Samples were submitted to the
Metabolomics Core at Mayo Clinic (Rochester, MN, USA) for metabolomics profiling
by liquid chromatography-mass spectrometry (LC/MS) using 6550 iFunnel Quadrupole
Time of Flight (Q-TOF) mass spectrometer (Agilent). Metabolites were then
identified using METLIN database with Mass Profiler Professional software
(Agilent). Annotated metabolites with a p value less than 0.05 and a fold change
greater than 2 were further analyzed using MetaboAnalyst network
explorer40 (link)(metabolite-metabolite interactions) and enrichment analysis (pathway associated
metabolite sets) tools. KEGG and HMDB identifiers were used in
MetaboAnalyst.
Arneson-Wissink P.C., Hogan K.A., Ducharme A.M., Samani A., Jatoi A, & Doles J.D. (2020). The wasting-associated metabolite succinate disrupts myogenesis and impairs skeletal muscle regeneration. JCSM rapid communications, 3(2), 56-69.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!