Podophyllotoxin (100 mg, J&K Chemical Ltd. Cat. 131142) was dissolved in dimethyl sulfoxide (DMSO) to a final concentration of 50 mM for storage. And the GV oocytes were cultured in 10–100 nM Podophyllotoxin in M16 medium (Sigma) at 37 °C in a humidified atmosphere with 5% CO2. The control oocytes were cultured in fresh M16 medium with the same concentration of DMSO. After different culture time, the mouse oocytes were collected for the fluorescence staining.
Podophyllotoxin
Manufactured by J&K Scientific
Sourced in China
Podophyllotoxin is a natural compound derived from the roots of the mayapple plant (Podophyllum species). It is a key starting material for the synthesis of various pharmaceutical compounds. Podophyllotoxin exhibits antimitotic properties, inhibiting cell division and proliferation. Its core function is as a chemical precursor in the production of other drugs and therapeutic agents.
Automatically generated - may contain errors
2 protocols using podophyllotoxin
1
Podophyllotoxin Induced Mouse Oocyte Staining
2
Tubulin-targeting Compound Evaluation
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Podophyllotoxin was bought from J&K Chemical Ltd. (Shanghai, China. Cat. 131142). Mouse monoclonal anti-α-tubulin-FITC antibody, Hoechst 33342 were purchased from Sigma (St Louis, MO, USA). Anti-gamma-tubulin antibody was from the Abcam (Cambridge, UK). Rabbit anti-p44/42 MAPK antibody was purchased from Cell Signaling Technology (Boston, MA).
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