20 both female and male mice, 2–14 months old were used in this study. We used heterozygote for DAT-Cre (Slc6a3tm1.1(cre)Bkmn; The Jackson Laboratory, 006660)49 , DAT-tTA (Tg(Slc6a3-tTA)2Kftnk; this study), VGluT3-Cre (Slc17a8tm1.1(cre)Hze; The Jackson Laboratory, 028534)50 (link), LSL-tdTomato (Gt(ROSA)26Sortm14(CAG-tdTomato)Hze; The Jackson Laboratory, 007914)51 (link), and Ai148D (B6.Cg-Igs7tm148.1(tetO-GCaMP6f, CAG-tTA2)Hze/J; The Jackson Laboratory, 030328)52 (link) transgenic lines. VGluT3-Cre lines crossed with LSL-tdTomato were used for experiments with DA sensor, without use of Cre recombinase. Seven mice (5 males and 2 females) were used for first-time learning, twelve mice (9 males and 3 females) were used for reversal learning, and five mice (4 males and 1 females) were used for repeated learning. Mice are housed on a 12 hr dark (7:00–19:00)/12 hr light (19:00–7:00) cycle. Experiments were performed in the dark period. Ambient temperature is kept at 75±5 F° and humidity is kept below 50%. All procedures were performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and approved by the Harvard Animal Care and Use Committee
Dat cre
Manufactured by Jackson ImmunoResearch
Sourced in Montenegro
DAT-Cre is a research tool used in genetic studies. It is a Cre recombinase transgene expressed under the control of the dopamine transporter (DAT) promoter. The core function of DAT-Cre is to facilitate site-specific DNA recombination in cells expressing the dopamine transporter.
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Lab products found in correlation
Chat cre, by Jackson ImmunoResearch (5 mentions)
Gad2 cre, by Jackson ImmunoResearch (5 mentions)
C57bl 6j, by Jackson ImmunoResearch (5 mentions)
Vgat cre, by Jackson ImmunoResearch (2 mentions)
Drd1a tdtomato, by Jackson ImmunoResearch (2 mentions)
Slc6a3tm1.1 cre bkmn, by Jackson ImmunoResearch (1 mentions)
Dat tta, by Jackson ImmunoResearch (1 mentions)
C57bl 6 wt, by Jackson ImmunoResearch (1 mentions)
Pv cre, by Jackson ImmunoResearch (1 mentions)
Meox2 cre, by Jackson ImmunoResearch (1 mentions)
Vglut2 ires cre mice, by Jackson ImmunoResearch (1 mentions)
Drd1 cre, by Jackson ImmunoResearch (1 mentions)
C57bi 6j, by Jackson ImmunoResearch (1 mentions)
Chat chr2, by Jackson ImmunoResearch (1 mentions)
Vglut2 cre, by Jackson ImmunoResearch (1 mentions)
Th cre, by Jackson ImmunoResearch (1 mentions)
Td tomato cre reporter ai14, by Jackson ImmunoResearch (1 mentions)
C57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
Dat cre mice, by Jackson ImmunoResearch (1 mentions)
Th cre mice, by Jackson ImmunoResearch (1 mentions)
19 protocols using dat cre
1
Genetic mouse models for optogenetic calcium imaging
2
Multimodal Behavioral Neuroscience Protocol
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Animal procedures were conducted in accordance with standards from the National Institutes of Health and under the approval of the Princeton University Institutional Animal Care and Use Committee.
Experimental animals for imaging were comprised of 20 mice (11 males and 9 females) cross bred between DAT::cre (Jackson Laboratory strain 006660; (Lammel et al. 2015 (link))) and the Ai148 GCaMP6f reporter line (Jackson Laboratory strain 030328; (Daigle et al. 2018 (link))), a cross extensively characterized previously (Engelhard et al. 2019 (link)). The above mice were housed with littermates until 2 weeks prior to imaging, at which time they were singly housed with enrichment materials (running wheel, nestlets). Stimulus mice and mice used for snRNA-sequencing were C57 / BL6 WT (Jackson Laboratory) males and females between the ages of 10 and 16 weeks. Stimulus mice were non-littermates of imaged animals. Stimulus animals were housed with their cage mates throughout the experiment. Food and water were given ad libitum, except during 2 experimental food deprivation days for both imaging mice and hunger state manipulated sequencing mice. Prior to and throughout experimental assays, experimental and stimulus animals were housed under a 12 H light-dark cycle with experiments exclusively taking place during the dark phase.
Experimental animals for imaging were comprised of 20 mice (11 males and 9 females) cross bred between DAT::cre (Jackson Laboratory strain 006660; (Lammel et al. 2015 (link))) and the Ai148 GCaMP6f reporter line (Jackson Laboratory strain 030328; (Daigle et al. 2018 (link))), a cross extensively characterized previously (Engelhard et al. 2019 (link)). The above mice were housed with littermates until 2 weeks prior to imaging, at which time they were singly housed with enrichment materials (running wheel, nestlets). Stimulus mice and mice used for snRNA-sequencing were C57 / BL6 WT (Jackson Laboratory) males and females between the ages of 10 and 16 weeks. Stimulus mice were non-littermates of imaged animals. Stimulus animals were housed with their cage mates throughout the experiment. Food and water were given ad libitum, except during 2 experimental food deprivation days for both imaging mice and hunger state manipulated sequencing mice. Prior to and throughout experimental assays, experimental and stimulus animals were housed under a 12 H light-dark cycle with experiments exclusively taking place during the dark phase.
3
Genetically Engineered Mouse Lines
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Adult mice of both sexes were used. For compatibility with other projects in the laboratory, the PV-Cre (Jackson 017320) and DAT-Cre (Jackson 006660) used were also heterozygous for the FLP-dependent tdTomato reporter line Ai65F (Daigle et al., 2018 (link)); obtained in this case by crossing the Cre− and FLP-dependent tdTomato double-reporter line Ai65D (Madisen et al., 2015 (link); Jackson Laboratory 021875) to the Cre deleter line Meox2-Cre (Tallquist and Soriano, 2000 (link); Jackson Laboratory 003755), then breeding out the Meox2-Cre allele, resulting in a reporter line for which only FLP is required for expression of tdTomato). For those mice in which RVΔG-4FLPo was used, the reporter allele was necessary for reporting RV activity; for those in which RVΔG-4mCherry was used, the presence of this reporter allele was irrelevant. For Cre-negative control injections using RVΔG-4FLPo, the Ai65F line was used. For Cre-negative control injections using RVΔG-4mCherry, either Ai65F or the Cre-dependent reporter line Ai14 (Madisen et al., 2010 (link)) was used; in these cases, the presence of the reporter alleles was again irrelevant.
4
Genetically Engineered Mice for Neurobehavioral Studies
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All experiments followed the guidelines for care and use of laboratory animals provided by the National Research Council, and with approved animal protocols from the Institutional Animal Care and Use Committee of the University of Massachusetts Chan Medical School (UMCMS). C57Bl/6J (Stock #000664, Jackson), GAD2Cre (Stock #10802, Jackson), DATCre (Stock #006660, Jackson), ChATCre (Stock #006410, Jackson) mice were bred in the UMMS animal facility and used in behavioral, optogenetic and fiber photometry experiments as indicated. Cre lines were crossed with C57Bl/6J mice and only heterozygous animals carrying one copy of the Cre recombinase gene were used for experimental purposes. For social experiments, juvenile stimuli always consisted of sex-matched C57Bl/6J mice (4–7 weeks old) bred in the UMCMS animal facility. All mice were housed together (21.3 °C and 49.9% humidity) and kept on a standard 12 h light/dark cycle (lights ON at 7 A.M.) with ad libitum access to food and water. Three to four weeks before experimentation, subject mice were kept under a reverse 12 h light/dark cycle (lights ON at 7 P.M.), and individually housed for at least 5 days before any behavioral testing. All experiments were performed during the dark cycle phase (8 A.M. to 5 P.M.).
5
Genetic Manipulation of Mice for Neuroscience
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Adult male DAT-Cre (The Jackson Laboratory, stock number 006660) and vGlut2-ires-Cre mice (The Jackson Laboratory, stock number 016963), weighing 20–25 g, were used for all experiments. Mice were kept on a 12/12 h light/dark cycle (lights on at 7 A.M., lights off at 7 P.M.) with ad libitum access to food and water. Mice had a minimum of three weeks to recover after surgery, and at least 3 d of rest were provided after each experiment. All animal procedures were reviewed and approved by the authors Animal Care and Use Committee.
6
Transgenic Mouse Models for Parkinson's
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αSyn KO mice on a C57BL/6N background (strain 016123, The Jackson Laboratory) (Baptista et al. 2013 (link)) were used for most experiments. Before these mice were available, αsyn KO mice on a mixed C57BL/6 and 129 × 1/SvJ background (strain 003692, The Jackson Laboratory, backcrossed one generation with C57BL/6N controls) were used for experiments outlined in Fig. 2B . DATcre (Backman et al. 2006 (link)) mice were also obtained from The Jackson Laboratory. C57BL/6N mice served as controls for all studies. Mice were group-housed in a colony maintained with a standard 12-h light/dark cycle and given food on the cage floor and water ad libitum. All experiments were performed on age- and sex-matched mice. Experiments were conducted in accordance with the Guide for the Care and Use of Laboratory Animals, as adopted by the National Institutes of Health and with approval of the Authors’ University Institutional Animal Care and Use Committee.
7
Optimized Mouse Husbandry for Behavioral Studies
8
Mouse Handling and Genotyping Protocol
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Mice were handled following the protocols approved by the Fudan University Animal Care and Use Committee. Six to ten weeks old male and female animals were used in this study. Following mouse lines were used: C57BL/6, D1-Cre (034258-UCD, MMRRC), DAT-Cre (#006660, Jackson Laboratory), Vglut2-Cre (#016963, Jackson Laboratory), and Vgat-Cre (#017535, Jackson Laboratory) mice. Genotyping were conducted following standard procedures on the Jackson Lab or MMRRC websites.
9
Mouse Strain Characterization Protocol
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Male and female C57Bl/6J (Jackson Laboratory, Bar Harbor, ME, Stock #000664) or DAT-Cre/Ai14-tdTomato (on a C57Bl/6J background) mice were used. The latter were generated in-house using DAT-Cre (Slc6a3tm1.1(cre)Bkmn – Jackson Laboratory, Stock #006660) and Ai14 (Gt(ROSA)26Sortm14(CAG-tdTomato)Hze – Jackson Laboratory, Stock #007908) mice. All experiments were performed in accordance with protocols reviewed and approved by the Northwestern Institutional Animal Care and Use Committee and NIH guidelines.
10
Mouse Behavioral and Optogenetic Experiments
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