Mindy1

Manufactured by Thermo Fisher Scientific

Sourced in United States

The MINDY1 is a compact and versatile lab equipment designed for various laboratory applications. It functions as a multipurpose tool for researchers and scientists. The core function of the MINDY1 is to provide a reliable and efficient platform for laboratory tasks.

Automatically generated - may contain errors

Lab products found in correlation

Protease inhibitor, by Merck Group (2 mentions) Ripa extraction reagent, by Beyotime (2 mentions) Gapdh, by Proteintech (2 mentions) Chemidoc xrs imaging system, by Bio-Rad (1 mentions) Ecl kit, by Boster Bio (1 mentions) 0.45 μm pvdf membrane, by Merck Group (1 mentions)

2 protocols using mindy1

Western Blot Analysis of Protein Expression

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Cells were lysed with RIPA extraction reagent (Beyotime, China) supplemented with protease inhibitors (Sigma-Aldrich, USA). Total protein was separated using 10–12.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to 0.45 μm PVDF membrane (Millipore, USA). Primary antibodies were YAP (Proteintech, 13584-1-AP), MINDY1 (Invitrogen, PA5-55825), HA (Proteintech, 51064-2-AP), Myc (Proteintech, 60003-2-Ig), and GAPDH (Proteintech, 60004-1-Ig) antibodies. Bands were visualized using an enhanced chemiluminescence (ECL) kit (Boster, China) and detected by ChemiDoc XRS + Imaging System (Bio-Rad).

Luo Y., Zhou J., Tang J., Zhou F., He Z., Liu T, & Liu T. (2021). MINDY1 promotes bladder cancer progression by stabilizing YAP. Cancer Cell International, 21, 395.

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Immunoblotting of Cellular Proteins

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Proteins were extracted from cultured cells using RIPA extraction reagent (Beyotime, China) supplemented with protease inhibitors (Sigma-Aldrich, USA), followed by immunoblotting with the corresponding antibodies: ERα (CST, 8644), Myc (Proteintech, 60003-2-Ig), HA (Proteintech, 51064-2-AP), MINDY1 (Invitrogen, PA5-55825), and GAPDH (Proteintech, GAPDH-Antibody-60004-1-Ig.htm">60004-1-Ig) antibodies.

Tang J., Luo Y., Long G, & Zhou L. (2021). MINDY1 promotes breast cancer cell proliferation by stabilizing estrogen receptor α. Cell Death & Disease, 12(10), 937.

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