Immpress anti rabbit ig

Manufactured by Vector Laboratories

Sourced in Canada, United States

The ImmPRESS™ anti-rabbit Ig is a peroxidase-based detection reagent designed for use in immunohistochemistry (IHC) and other immunoassay applications. It is formulated to specifically detect rabbit immunoglobulins.

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Lab products found in correlation

Anti aβ, by Agilent Technologies (2 mentions) Anti phospho tdp 43 ps409 410, by Cosmo Bio (2 mentions) Immpress anti mouse igg, by Vector Laboratories (2 mentions) Sk 4100, by Vector Laboratories (2 mentions) Immpress anti rat ig, by Vector Laboratories (2 mentions) Dab peroxidase substrate kit, by Vector Laboratories (2 mentions) Plan apochromat 20 0.8 objective, by 3DHISTECH (1 mentions) D4w2z, by Cell Signaling Technology (1 mentions) Hematoxylin, by Vector Laboratories (1 mentions) Mouse adsorbed peroxidase polymer detection kit, by Vector Laboratories (1 mentions) Normal horse serum, by Vector Laboratories (1 mentions) Bloxall, by Vector Laboratories (1 mentions) Immpact dab, by Vector Laboratories (1 mentions) Vectamount, by Vector Laboratories (1 mentions)

5 protocols using immpress anti rabbit ig

Cellular-Level Autoradiography and Immunohistochemistry

Cited 1 time

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To obtain autoradiographic information at the cellular resolution level, frozen cryostat sections, adjacent to those used for phosphor screen autoradiography, were coated with a liquid photographic emulsion following our previously published protocol [5 (link), 9 (link), 22 (link), 34 (link)]. Immunohistochemistry was then performed on the nuclear emulsion-dipped sections. First the sections were washed for 5 min with PBS, then incubated with 2.5% normal horse blocking serum for 20 min, followed by the appropriate primary antibody - anti-tau PHF-1 (1:100, mouse, kind gift of Dr. Peter Davies), anti-Aβ (1:500, mouse, clone 6F/3D, Dako), anti α-synuclein (1:100, mouse, Zymed) or anti-phospho TDP-43 (pS409/410) (1:3000, mouse, Cosmo Bio CO) - for 40 min at 37 °C, washed with PBS twice for 2 min, and then incubated with the secondary antibody (ImmPRESS™ anti-mouse IgG (Vector Laboratories product MP-2400, Burlingame, CA) or ImmPRESS™ anti-rabbit Ig (Vector Laboratories product MP-7401, Burlingame, CA)) for 40 min at 37 °C. The sections were washed again with PBS twice for 2 min, and developed with DAB solution (Vector Laboratories product SK-4100). H&E was used for counterstaining. Photomicrographs were obtained on an upright Olympus BX51 (Olympus, Denmark) microscope using visible light.

Aguero C., Dhaynaut M., Normandin M.D., Amaral A.C., Guehl N.J., Neelamegam R., Marquie M., Johnson K.A., El Fakhri G., Frosch M.P, & Gomez-Isla T. (2019). Autoradiography validation of novel tau PET tracer [F-18]-MK-6240 on human postmortem brain tissue. Acta Neuropathologica Communications, 7, 37.

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Immunohistochemical Analysis of Liver Tumor

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Paraffin-embedded liver/tumor
tissue sections were deparaffinized, rehydrated, underwent heat-induced
antigen retrieval, and then incubated with primary Abs. The primary
Abs types used for detection of Ki-67, F4/80, Gr-1, and CD8alpha were
SP6 (Abcam), BM8 (Biolegend), RB6F8C5 (Biolegend), and D4W2Z (Cell
Signaling Technology), respectively. ImmPRESS anti-rat Ig, ImmPRESS
anti-rabbit Ig, polymer detection kits, DAB peroxidase substrate kit,
(Vector laboratories) liquid permanent red substrate (Dako), and Hematoxylin
Gill II (Leica) were used for detection and visualization. The images
were captured using an automatic digital slide scanner Pannoramic
MIDI with a Plan-Apochromat 20×/0.8 objective (3D HISTECH) by
the Pathology Core Laboratory of NHRI.

Lo C.F., Chiu T.Y., Liu Y.T., Pan P.Y., Liu K.L., Hsu C.Y., Fang M.Y., Huang Y.C., Yeh T.K., Hsu T.A., Chen C.T., Huang L.R, & Tsou L.K. (2022). Targeting the Phosphatidylserine-Immune Checkpoint with a Small-Molecule Maytansinoid Conjugate. Journal of Medicinal Chemistry, 65(19), 12802-12824.

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Immunohistochemical Analysis of Liver and Tumor Tissues

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Paraffin-embedded liver/tumor tissue sections were deparaffinized, rehydrated, followed by heat-induced antigen retrieval and then incubated with primary antibodies as listed in Additional file 1: Table S1. ImmPRESS anti-Rat Ig, ImmPRESS anti-Rabbit Ig, Mouse adsorbed (peroxidase) Polymer Detection Kit, DAB Peroxidase Substrate Kit and Hematoxylin (all from Vector laboratories, Burlingame, USA) were used for detection and visualization. For detection of CD45.1⁺ adoptively transferred CD8⁺ T cells, 5–7 μm of liver/tumor tissue frozen sections were fixed in ice cold acetone/chloroform (1:1), incubated with FITC-conjugated anti-CD45.1 (A20, Biolegend) and anti-FITC conjugate HRP (Thermo Fisher Scientific, Waltham, USA), followed by incubation with DAB substrate. For detection of lipid droplets, 5–7 μm liver/tumor tissue frozen sections were fixed in 10% formalin, incubated with oil red O dye for 15 min and washed with 60% isopropanol to remove the excess dye before mounting with glycerol jelly mounting medium. The images were captured using an automatic digital slide scanner Pannoramic MIDI with Plan-Apochromat 20x/0.8 objective (3D HISTECH) by Pathology Core Laboratory of NHRI and have been evaluated by an experienced pathologist (SFH).

Liu Y.T., Tseng T.C., Soong R.S., Peng C.Y., Cheng Y.H., Huang S.F., Chuang T.H., Kao J.H, & Huang L.R. (2018). A novel spontaneous hepatocellular carcinoma mouse model for studying T-cell exhaustion in the tumor microenvironment. Journal for Immunotherapy of Cancer, 6, 144.

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Quantitative Immunohistochemistry of Arterial α1D-Adrenergic Receptors

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Femoral arteries were resected, formalin fixed, and mounted longitudinally for sectioning. 30 μm sections were made along the length of the artery and placed in 10% formalin overnight. Sections were rinsed in distilled water to remove residual formalin. Endogenous peroxidase activity was removed with Bloxall (Vector Laboratories, Burlingame, CA, SP-6000). 2.5% normal horse serum (Vector Laboratories, Burlingame, CA, S-2012) was then used as a blocking reagent followed by primary antibody incubation with α_1D antibody (1:200, sc-10721, Santa Cruz Biotechnology, Santa Cruz, CA) for 90 minutes. Sections were incubated for 30 minutes in ImmPRESS anti-rabbit Ig (Vector Laboratories, Burlingame, CA, MP-7401) and ImmPACT DAB (Vector Laboratories, Burlingame, CA, SK-4105) was used for staining. Sections were mounted using Vectamount (Vector Laboratories, Burlingame, CA, H-5000). Bright-field imaging was carried out at 10X objective magnification. Images were digitized to 8-bit format and measurements were taken of smooth muscle regions in each section (6 measurements/image, 10 images/animal). All analysis was performed on ImageJ. Precautions were taken to assure that all IHC steps were carried out under standard conditions to allow quantitative comparison with digital image analysis.

Santamaria M., Aletti F., Li J.B., Tan A., Chang M., Leon J., Schmid-Schönbein G.W, & Kistler E.B. (2017). Enteral tranexamic acid attenuates vasopressor resistance and changes in α1-adrenergic receptor expression in hemorrhagic shock. The journal of trauma and acute care surgery, 83(2), 263-270.

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Immunohistochemistry for Neurodegenerative Markers

Cited 3 times

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To gain resolution at the cellular level, adjacent slides to those used in the phosphor screen autoradiography experiments, were dipped in a liquid photographic emulsion following our previously published protocols[1 (link), 29 (link), 31 (link)], followed by immunohistochemistry using the appropriate primary and secondary antibodies (primary antibodies used were: anti-tau PHF-1 (1:100, mouse, kind gift of Dr. Peter Davies), anti-Aβ (1:500, mouse, clone 6F/3D, Dako), anti α-synuclein (1:100, mouse, Zymed) and anti-phospho TDP-43 (pS409/410) (1:3000, mouse, Cosmo Bio CO); secondary antibodies used were ImmPRESS™ anti-mouse IgG (Vector Laboratories product MP-2400, Burlingame, CA) or ImmPRESS™ anti-rabbit Ig (Vector Laboratories product MP-7401, Burlingame, CA)) and developed with DAB solution (Vector Laboratories product SK-4100). H&E was used for counterstaining. Photomicrographs were obtained on an upright Olympus BX51 (Olympus, Denmark) microscope using visible light.

Aguero C., Dhaynaut M., Amaral A.C., Moon S.H., Neelamegam R., Scapellato M., Carazo-Casas C., Kumar S., El Fakhri G., Johnson K., Frosch M.P., Normandin M.D, & Gómez-Isla T. (2024). Head-to-head comparison of [18F]-Flortaucipir, [18F]-MK-6240 and [18F]-PI-2620 postmortem binding across the spectrum of neurodegenerative diseases. Acta Neuropathologica, 147(1), 25.

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