Anti fbw7

Manufactured by Abcam

Sourced in United Kingdom

Anti-FBW7 is a laboratory reagent used for the detection and analysis of the FBW7 protein. FBW7 is a component of the SCF ubiquitin ligase complex and plays a role in the regulation of cell cycle and cell proliferation. The Anti-FBW7 product provides a tool for researchers to investigate the expression and function of FBW7 in various biological systems.

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Lab products found in correlation

Anti snail, by Abcam (1 mentions) Anti ubiquitin, by Merck Group (1 mentions) Anti tgf β, by Merck Group (1 mentions) Enhanced chemiluminescence ecl kit, by GE Healthcare (1 mentions) Anti actin antibody, by Merck Group (1 mentions) Anti cullin 1, by Abcam (1 mentions) Anti myc tag, by Cell Signaling Technology (1 mentions) Anti lc3b 2, by Cell Signaling Technology (1 mentions) Anti skp2 antibodies, by Thermo Fisher Scientific (1 mentions) Anti nedd8, by Abcam (1 mentions) Quantitative reverse transcription polymerase chain reaction rt pcr kit, by Bio-Rad (1 mentions) Reverse transcription system kit, by Promega (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) Anti p27kip1 antibody, by BD (1 mentions) Anti cdh1, by Cell Signaling Technology (1 mentions) Mg132, by Cayman Chemical (1 mentions) Mln4924, by Cayman Chemical (1 mentions)

2 protocols using anti fbw7

Quantitative Protein Analysis in Cardiac Fibrosis

Cited 1 time

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The total protein was extracted from the cultured CFs, and the levels were compared by immunoblotting the proteins based on their expression in the left ventricular peri-infarct area (PIA) of rats [32 (link)]. Antibodies used for probing were anti-FBW7, anti-Snail, anti-SMAD3/4, anti-phosphor-SMAD3/4 (Abcam, Cambridge, UK), anti-Twist, anti-TGF-β, anti-ubiquitin, and anti-actin antibody (Sigma-Aldrich Corp. St. Louis, MO, USA). The corresponding secondary antibodies were purchased from Stressgen Biotechnologies Corporation, Victoria, BC, Canada. Signals were used for detection using the Enhanced Chemiluminescence (ECL) kit (GE Healthcare, UK). Densitometry was employed to quantify the protein levels. β-actin was used as the loading control in the western blotting experiment for normalization.

Fu Q., Lu Z., Fu X., Ma S, & Lu X. (2019). MicroRNA 27b promotes cardiac fibrosis by targeting the FBW7/Snail pathway. Aging (Albany NY), 11(24), 11865-11879.

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Gartanin-Mediated Protein Degradation

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Gartanin was obtained from Chromadex with 99% purity (Irvine, CA). MG-132 and MLN4924 were obtained from Cayman Chemical Inc. (Ann Arbor, MI). Antibodies against Ubc12, ubiquitin, and β-tubulin were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Anti-Skp2 antibodies were purchased from Invitrogen (Grand Island, NY). Anti-Myc-tag, anti-LC3B-II, anti-FBXW2, anti-Cdh1, and anti-β-TrPC antibodies were from Cell Signaling Inc. (Boston, MA). Anti-Cullin-1, anti-FBW7 and anti-NEDD8 antibodies were from Abcam (Cambridge, MA). Anti-p27/Kip1 antibody was from BD Biosciences (Billerica, MA). 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) was purchased from Sigma. Reverse Transcription System kit and was from Promega (Mandison, WI). A quantitative reverse transcription polymerase chain reaction (RT-PCR) kit was from Bio-Rad (Hercules, CA).

Pham V., Rendon R., Le V.X., Tippin M., Fu D.J., Le T.H., Miller M., Agredano E., Cedano J, & Zi X. (2019). Gartanin is a novel NEDDylation inhibitor for induction of Skp2 degradation, FBXW2 expression and autophagy. Molecular carcinogenesis, 59(2), 193-201.

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