Anti cd14 clone t k4

Manufactured by Miltenyi Biotec

Sourced in Belgium

The Anti-CD14 (clone TÜK4) is a monoclonal antibody that binds to the CD14 antigen. CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein that serves as a co-receptor for the detection of bacterial lipopolysaccharide (LPS). The Anti-CD14 (clone TÜK4) can be used to identify and enumerate CD14-positive cells in various applications.

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Lab products found in correlation

Anti armenian hamster igg, by Jackson ImmunoResearch (1 mentions) Hu fcr binding inhibitor, by Thermo Fisher Scientific (1 mentions) Anti cd11b m1 70, by BioLegend (1 mentions) Anti f4 80 bm8, by Thermo Fisher Scientific (1 mentions) Facscan flow cytometer, by BD (1 mentions) Facscan, by BD (1 mentions) Cellquest pro software, by BD (1 mentions)

2 protocols using anti cd14 clone t k4

Comprehensive Immune Cell Profiling Protocol

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RPMI 1640 was obtained from Corning Technologies. Antibiotics were
purchased from Sigma. FBS purchased from Hyclone. Dead cells were excluded using
Invitrogen Fixable LIVE/DEAD in Near-IR, Yellow, or Violet. The following
antibodies were used for flow cytometry or immunofluorescence staining: from
BioLegend: anti-VISTA (clone MH5A), anti-CD45 (30-F11), anti-CD11b (M1/70),
anti-CD4 (RM4–5), anti-CD8 (53–6.7), anti-Ly6C (HK1.4), anti-Ly6G
(IA8), anti-Gr1 (RB6–8C5), anti-F4/80 (BM8), and Armenian Hamster IgG
Isotype control (HTK888); from eBioscience: anti-CD11c (N418), anti-CD16/CD32
(clone 93), and anti-FoxP3 (FJK-16s); anti- Armenian Hamster IgG (Jackson
ImmunoResearch), and anti-VISTA (clone 13F3, made in-house). Antibodies for flow
cytometry staining of human PBMCs: Hu FcR Binding Inhibitor (eBioscience),
anti-VISTA (GG8, made in-house), anti-CD14 (clone TÜK4, Miltenyi), and
from BioLegend: anti-CD11b (M1/70), anti-CD33 (WM-53), anti-HLA-DR (L243),
anti-CD3 (SK7), anti-CD19 (HIB19), and mouse IgG₁ κ isotype
control (MOPC-21). For blocking experiments, antibodies used were: anti-VISTA
(clone 13F3, made in-house) and Armenian Hamster IgG1 Isotype Control (clone
PIP, BioXCell).

Deng J., Li J., Sarde A., Lines J.L., Lee Y.C., Qian D.C., Pechenick D.A., Manivanh R., LeMercier I., Lowrey C.H., Varn F.S., Cheng C., Leib D.A., Noelle R.J, & Mabaera R. (2019). Hypoxia-induced VISTA promotes the suppressive function of myeloid- derived suppressor cells in the tumor microenvironment. Cancer immunology research, 7(7), 1079-1090.

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Monocyte Subpopulation Analysis by Flow Cytometry

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Monocytes were stained using monoclonal antibodies against CD14 which is a monocytic differentiation antigen and part of the LPS receptor complex [phycoerythrine (PE) labelled anti-CD14 (clone TÜK4), Miltenyi Biotec] and against the Fcγ receptor, CD16 [fluorescent isothiocyanate (FITC) labelled anti-CD16 (clone VEP13), Miltenyi Biotec] and analysed by flow cytometry (FACScan, Becton Dickinson, Erembodegem, Belgium). Cell populations were analysed with the FACScan flow cytometer and the CellQuestPro software (Becton Dickinson).
Monocytes in this part of the study were subdivided into 3 distinct subpopulations, based on the expression levels of CD14 and CD16 (CD14⁺⁺CD16^-; CD14⁺⁺CD16⁺; CD14⁺CD16⁺⁺) indicated by fluorescence intensity values.

Schepers E., Houthuys E., Dhondt A., De Meyer G., Neirynck N., Bernaert P., Van den Bergh R., Brouckaert P., Vanholder R, & Glorieux G. (2015). Transcriptome Analysis in Patients with Chronic Kidney Disease on Hemodialysis Disclosing a Key Role for CD16+CX3CR1+ Monocytes. PLoS ONE, 10(4), e0121750.

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