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Ship2

Manufactured by Cell Signaling Technology
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SHIP2 is a lab equipment product that functions as a protein phosphatase. It catalyzes the dephosphorylation of phosphoinositide-3,4,5-trisphosphate (PIP3) to phosphoinositide-3,4-bisphosphate (PIP2).

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Lab products found in correlation

P akt ser473, by Cell Signaling Technology (2 mentions) P akt thr308, by Cell Signaling Technology (2 mentions) Protease inhibitor, by Merck Group (1 mentions) Vinculin, by Merck Group (1 mentions) Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions) Bcl w, by Cell Signaling Technology (1 mentions) Bcl xl, by Cell Signaling Technology (1 mentions) Mcl 1, by Cell Signaling Technology (1 mentions) Pras40, by Cell Signaling Technology (1 mentions) Ps6 ser235 236, by Cell Signaling Technology (1 mentions) P pras40 thr246, by Cell Signaling Technology (1 mentions) Histone h3, by Cell Signaling Technology (1 mentions) Cycloheximide chx solution, by Merck Group (1 mentions) Siah2, by Proteintech (1 mentions) Ubiquitin, by Proteintech (1 mentions) β actin, by Proteintech (1 mentions) Nedd4, by Proteintech (1 mentions) Cul4a, by Proteintech (1 mentions) Mg132, by Beyotime (1 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)

3 protocols using ship2

1

Western Blot Analysis of AKT/mTOR Pathway

Cited 1 time
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Western blots were performed as described previously [52 (link)]. Briefly, cells were lysed in RIPA buffer supplemented with phosphatase (Sigma–Aldrich) and protease inhibitors (Sigma–Aldrich) and equal amounts of protein were loaded and separated by SDS-PAGE and transferred onto a PVDF membrane. Membranes were probed with primary antibody then with appropriate horseradish peroxidase-conjugated secondary and incubated with ECL. Antibodies obtained from Cell Signalling: AKT-T (#9272), pAKT-Ser473 (#4060), pAKT-Thr308 (2965), S6 (#2217), pS6-Ser235/236 (#2111), PRAS40 (#2691), pPRAS40-Thr246 (#13175), TSC2 (#4308), PARP (#9532, #9542), MCL1 (#9429), BAD-T (#9239), BAD-P Ser 136 (#4366), Bcl-XL (#2764), Bcl-w (#2724), BAX (#5023, #2772), BAK (#12105, #6947), Bim (#2933), BID (#2002), Puma (#4976), SHIP2 (#2839). Other antibodies used were Bcl-2 (ab32124, Abcam), and HRK (#ab45419, Abcam) Actin (A2228, Sigma), Vinculin (#V9132, Sigma) PIK3R2 (#A302-593A, Bethyl Laboratories), Caspase-3 (#14220). Secondary antibodies used were HRP-linked anti-rabbit IgG (CST#7074,1:2000) and HRP-linked anti-mouse IgG (CST#7076, 1:2000).
Dunn S., Eberlein C., Yu J., Gris-Oliver A., Ong S.H., Yelland U., Cureton N., Staniszewska A., McEwen R., Fox M., Pilling J., Hopcroft P., Coker E.A., Jaaks P., Garnett M.J., Isherwood B., Serra V., Davies B.R., Barry S.T., Lynch J.T, & Yusa K. (2022). AKT-mTORC1 reactivation is the dominant resistance driver for PI3Kβ/AKT inhibitors in PTEN-null breast cancer and can be overcome by combining with Mcl-1 inhibitors. Oncogene, 41(46), 5046-5060.
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2

Antibody Sources for Cell Signaling

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Antibodies against PHB2 (Cat#14085S), PHB1 (Cat#2426S), SHIP2 (Cat#2839S), Akt (Cat#9272S), pAkt-Ser473 (Cat#4060S), pAkt-Thr308 (Cat#13038S), p21 (Cat#2947S), p27 (Cat#3686S), Histone H3 (Cat#9715S), K63 (Cat#5621S), K48 (Cat#8081S), and Ki67 (Cat#34330SF) were purchased from Cell Signaling Technology (Beverly, MA, USA), antibodies against tGFP (Cat#TA150041) was purchased from OriGene (Rockville, MD, USA), antibodies against Flag (Cat#66008-3-Ig, Cat#20543-1-AP), HA (Cat#51064-2-AP, Cat#66006-2-Ig), His (Cat#66005-1-Ig), GST (Cat#66001-2-Ig), Ubiquitin (Cat#10201-2-AP), β-actin (Cat#66009-1-Ig), NEDD4 (Cat#21698-1-AP), SIAH2 (Cat#12651-1-AP), WWP2 (Cat#67274-1-Ig), Cul4A (Cat#14851-1-AP) and MUL1 (Cat#16133-1-AP) were purchased from Proteintech (Wuhan, China). MG132 (Cat#S1748) was purchased from Beyotime Biotechnology (Shanghai, China). Cycloheximide (CHX) solution was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Xu L., Xiang W., Yang J., Gao J., Wang X., Meng L., Ye K., Zhao X.H., Zhang X.D., Jin L, & Ye Y. (2024). PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer. Journal of Experimental & Clinical Cancer Research : CR, 43, 17.
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3

Inhibitor-based Signaling Pathway Analysis

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Growth factors were purchased from Life Technologies, Carlsbad, CA (EGF), and PeproTech, Rocky HIll, NJ (HGF, NRG-1, and IGF). The compounds used were Met inhibitor SU11274 (Selleck Chemicals, Houston, TX), EGFR inhibitor erlotinib (LC Labs, Woburn, MA; PeproTech), PTP1B inhibitor (539741; Calbiochem, Billerica, MA), and SHIP2 inhibitor AS1949490 (Tocris, Minneapolis, MN). The following antibodies were used: EGFR (Cell Signaling Technology, Danvers, MA), pEGFR Y1173 (Cell Signaling Technology; Epitomics), vimentin (BD Transduction Laboratories, San Jose, CA), E-cadherin (Cell Signaling Technology), PTP1B (Millipore, Burlington, MA), SHIP2 (Cell Signaling Technology), and panMena (Lebrand et al., 2004 (link)).
Hughes S.K., Oudin M.J., Tadros J., Neil J., Del Rosario A., Joughin B.A., Ritsma L., Wyckoff J., Vasile E., Eddy R., Philippar U., Lussiez A., Condeelis J.S., van Rheenen J., White F., Lauffenburger D.A, & Gertler F.B. (2015). PTP1B-dependent regulation of receptor tyrosine kinase signaling by the actin-binding protein Mena. Molecular Biology of the Cell, 26(21), 3867-3878.
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