Cpd 300 auto critical point dryer

SEM analyses were performed on plastics after removing the biofilm and without removing the biofilm. Samples for SEM were incubated in 2% glutaraldehyde for 10 min and then stored at −80°C until further processing. SEM samples were dehydrated with an ethanol dilution series of 30%, 50%, 70%, 80%, 90%, and 95% each for 10 min and three times in 100% absolute ethanol for 10 min. The dehydrated samples were CO₂ critical-point dried with a CPD 300 auto-critical-point dryer (Leica Microsystems). The dried pieces were gold coated using a JFC-2300HR sputter coater (JEOL Ltd.) for 80 s. Pictures were taken using a secondary electron detector with a JEOL JSM-IT300 scanning electron microscope with 15-kV acceleration voltage in ultrahigh vacuum.

SEM samples were dehydrated in an ethanol dilution series of 30%, 50%, 70%, 80%, 90%, 95% each for 10 min and three times in 100% absolute ethanol for 10 min. The dehydrated samples were CO₂ critical point dried with a CPD 300 auto critical point dryer (Leica Microsystems). The dried pieces were gold-coated using a JFC-2300HR sputter coater (JEOL Ltd.) for 80s. Pictures were taken using a secondary electron detector with a JEOL JSM-IT300 scanning electron microscope at 150x, 450x, 2000x magnification, 15kV acceleration voltage in ultra-high vacuum.