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Gemini xps dual scanning microplate spectrofluorometer

Manufactured by Molecular Devices
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The Gemini XPS Dual‐Scanning Microplate Spectrofluorometer is a laboratory instrument designed for fluorescence-based measurements. It features dual-scanning capabilities, allowing for the simultaneous detection of multiple samples in microplate format.

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Lab products found in correlation

Quant it protein assay kit, by Thermo Fisher Scientific (2 mentions) Softmax pro software, by Molecular Devices (2 mentions)

Spelling variants of this product

Gemini XPS (35 citations) Spectrofluorometer (18 citations) Microplate spectrofluorometer (7 citations) Gemini XPS microplate spectrofluorometer (5 citations) Gemini XPS spectrofluorometer (3 citations)

2 protocols using gemini xps dual scanning microplate spectrofluorometer

1

Protein Quantification Using Fluorescence

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Cells were counted in a hemocytometer with trypan blue. Total protein concentrations were determined using the fluorescent Quant‐iT™ protein assay kit (Invitrogen/Molecular Probes, Eugene, OR) with bovine serum albumin (0–500 ng/mL) as a standard for quantification. Fluorescence (excitation at 470 nm and emission at 570 nm) was measured using a Gemini XPS Dual‐Scanning Microplate Spectrofluorometer and data were analyzed using SoftMax® Pro software (Molecular Devices, Sunnyvale, CA).
Cowan R.P., Gross N.B., Sweeney M.D., Sagare A.P., Montagne A., Arakaki X., Fonteh A.N., Zlokovic B.V., Pogoda J.M, & Harrington M.G. (2021). Evidence that blood–CSF barrier transport, but not inflammatory biomarkers, change in migraine, while CSF sVCAM1 associates with migraine frequency and CSF fibrinogen. Headache, 61(3), 536-545.
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2

Cell Counting and Protein Quantification

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Cells were counted in a hemocytometer with trypan blue. Total protein concentrations were determined using the fluorescent Quant-iT™ protein assay kit (Invitrogen/Molecular Probes, Eugene, OR) with bovine serum albumin (0–500 ng/mL) as a standard for quantification. Fluorescence (excitation at 470 nm and emission at 570 nm) was measured using a Gemini XPS Dual-Scanning Microplate Spectrofluorometer and data were analyzed using SoftMax® Pro software (Molecular Devices, Sunnyvale, CA).
Cowan R.P., Gross N.B., Sweeney M.D., Sagare A.P., Montagne A., Arakaki X., Fonteh A.N., Zlokovic B.V., Pogoda J.M, & Harrington M.G. (2021). Evidence that blood–CSF barrier transport, but not inflammatory biomarkers, change in migraine, while CSF sVCAM1 associates with migraine frequency and CSF fibrinogen. Headache, 61(3), 536-545.
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