G6PD enzyme activity was measured by a quantitative spectrophotometric assay based on the evaluation of absorbance at 340 nm given by NADPH formation. Whole blood samples were collected into tubes containing EDTA-K2. The haemogram analysis was done using an automated hematology analyzer (Beckman Coulter, USA). After purified erythrocytes were lysed, the G6PD activity was determined by commercially available kits (Guangzhou Kofa Biotechnology, Guangdong, China) on Hitachi Model 7600 Series Automatic Analyzer (Hitachi High-Technologies Corporation, Ibaraki, Japan) and AU5800 automatic biochemistry analyzer (Beckman Coulter, USA). The operations were followed by the manufacturer’s instructions. The cutoff value for G6PD deficiency was set at 10.0 IU/g Hb according to the manufacturer’s recommendation. All samples were assayed within 24 h after sample collection to prevent the reduction of G6PD activity. Samples in the presence of obvious hemolysis, abnormal erythrocyte count were excluded39 (link). G6PD activity was finally expressed as the ratio of G6PD units per gram of Hb.
Model 7600 series automatic analyzer
Manufactured by Hitachi
Sourced in Japan
The Hitachi Model 7600 Series Automatic Analyzer is a laboratory equipment designed for automated chemical analysis. It performs various analytical procedures, including colorimetric, enzymatic, and ion-selective measurements. The device is capable of handling a wide range of sample types and can be configured with multiple testing modules to meet the specific requirements of different laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Aia 360, by Tosoh (3 mentions)
Immulite 2000 xpi, by Siemens (2 mentions)
Surestep blood glucose meter, by LifeScan (2 mentions)
D 10 hemoglobin testing program, by Bio-Rad (2 mentions)
Automated hematology analyzer, by Beckman Coulter (1 mentions)
Au5800 automatic biochemistry analyzer, by Beckman Coulter (1 mentions)
Synchron cx5 auto analyzer, by Beckman Coulter (1 mentions)
Ca 7000 system coagulation analyzer, by Sysmex (1 mentions)
Aeroset automated analyzer, by Abbott (1 mentions)
Xe 2100 analyzer, by Sysmex (1 mentions)
Lightcycler 480 system, by Roche (1 mentions)
Architect i2000sr, by Abbott (1 mentions)
Cobas e601, by Roche (1 mentions)
Hlc 723g8 automatic glycosylated hemoglobin analyzer, by Tosoh (1 mentions)
Cobas e801, by Roche (1 mentions)
D 10 testing program, by Bio-Rad (1 mentions)
13 protocols using model 7600 series automatic analyzer
1
Quantitative Spectrophotometric Assay of G6PD Activity
2
COVID-19 Biomarker Profiling in Blood
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Venous blood samples (4 mL) were obtained from all persons who tested positive to a COVID-19 PCR test and were divided into two aliquots: one aliquot was anticoagulated and divided into two test tubes. The first one was for hematological examinations of total leucocytes count (WBCs) using a Hema Screen 18-Automated Haematology Analyser (Hospitex Diagnostics, Sesto Fiorentino, Italy). The second test tube contained plasma separated for d -dimer with an Sysmex® CA-7000 system coagulation analyzer (Sysmex, Kobe, Japan), and detection of hs-C- reactive protein (CRP) with a Hitachi Model 7600 Series Automatic Analyzer (Hitachi High Technologies Corporation, Hitachi, Japan). The kits used in the experiments were d -dimer PLUS (Siemens Healthcare Diagnostics Products GmbH) and reagent kit for hs-CRP test (latex agglutination assay). Their reference values were 0.1417 (90% CL 0.00–0.55) µg/mL for d -dimer, and < 6 mg/L for hs-CRP, respectively.
From other aliquots of blood, samples could clot, and sera were then separated by centrifugation (3,500 rpm, 20 min, 25 °C) and stored at −20 °C for later biochemical determinations (serum creatinine and lactate dehydrogenase (LDH)). Plasma levels of total cholesterol, LDL-C, HDL-C, triacyclglycerols, and phospholipids were estimated using Synchron cx5 autoanalyzer (Beckman, USA) and calculated with the Friedewald formula.
From other aliquots of blood, samples could clot, and sera were then separated by centrifugation (3,500 rpm, 20 min, 25 °C) and stored at −20 °C for later biochemical determinations (serum creatinine and lactate dehydrogenase (LDH)). Plasma levels of total cholesterol, LDL-C, HDL-C, triacyclglycerols, and phospholipids were estimated using Synchron cx5 autoanalyzer (Beckman, USA) and calculated with the Friedewald formula.
3
Comprehensive Biomarker Profiling in Patients
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Serum ferritin, serum total prostate-specific antigen (total PSA), and free prostate-specific antigen (free PSA) were measured using chemiluminescence immunoassays (ARCHITECT-i4000 immunology analyzer, Abbott Laboratories, Irving, TX). Lipid parameters, including triglycerides, total cholesterol, high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, and very low-density lipoprotein (VLDL) cholesterol, were measured using a Hitachi Model 7600 Series Automatic Analyzer (Hitachi High-Technologies Corporation, Tokyo, Japan) in accordance with the manufacturer's instructions. This analyzer was also used to measure uric acid, fasting plasma glucose, and liver function parameters, including alanine transaminase (ALT), aspartate transaminase (AST), gamma-glutamyl transferase (GGT), direct bilirubin (DBIL), and indirect bilirubin (IBIL), using the respective reagents. Hemoglobin was measured using an XE2100 analyzer (Sysmex, Kobe, Japan). Serum CRP was measured using the latex agglutination method with an Abbott Aeroset automated analyzer. Blood pressure was measured using a mercury sphygmomanometer placed on the patient's right arm while at rest.
4
Plasma Homocysteine Measurement Protocol
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At the 48th week, the fasting animals were bled from the retroorbital plexus under aether anesthesia. Blood samples were obtained in chilled EDTA-containing microtubes and immediately centrifuged at 3000 rpm for 10 minutes at 4°C to limit the release of homocysteine (Hcy) from blood cells. Plasma was then stored at −80°C. Plasma Hcy concentration was measured by enzymatic cycling assay by Hitachi Model 7600 Series Automatic Analyzer (Hitachi High-Technologies Corporation, Japan).
5
Comprehensive Serum Biochemistry Assessment
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The routine serum biochemical tests were ordered for all the patients after admission and during treatment. These tests included serum alanine aminotransferase (ALT), total bilirubin (TBIL), ALB, international normalized ratio (INR) of blood coagulation, prothrombin time (PT), prothrombin activity (PTA), creatinine (Cr), and electrolytes (Hitachi Model 7600 Series Automatic Analyzer, Japan). Viral hepatitis markers for hepatitis A, B, C, Delta, and E viruses (ARCHITECT i2000SR, Abbott Laboratories Ltd, USA) as well as the antibodies for autoimmune liver diseases were examined. At the same time, HBV DNA levels were quantitatively tested for patients with positive serum hepatitis B surface antigen (HBsAg) (LightCycler 480 System, Roche, Penzberg, Germany). The serum AFP was respectively examined in all patients during the first seven days and after 14-28 days of treatment by electrochemiluminescence assay (Roche Cobas E 601).
6
Assessing Insulin Resistance and Ghrelin Levels
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Insulin concentration was examined by electrochemiluminescence immunoassay (IMMULITE2000 XPi, Siemens, Germany). Other biochemical indices were detected by an automatic biochemical analyzer (Model 7600 Series Automatic Analyzer, Hitachi, Japan). Insulin resistance was quantified using HOMA-IR [fasting insulin (mU/L) × fasting glucose (mmol/L)]/22.5. Plasma acylated ghrelin (AG) and desacyl ghrelin (DAG) levels were determined using ELISA kits (Human acylated and deacylated Ghrelin EIA Kits, Bertin Corporation, reference range: 2–250 pg/mL; intraassay coefficient of variation <5%; interassay coefficient of variation <15%). These tests were done following the manufacturer’s instruction manual. AG/DAG ratio was calculated as AG divided by DAG.
7
Comprehensive Metabolic Profiling Protocol
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Body mass index (BMI) was calculated (kg/m2). SBP and DBP were taken three times using a sphygmomanometer and then were averaged. Capillary glucose concentrations were measured with Lifescan Surestep blood glucose meter. Plasma glucose levels were measured using the glucose oxidase method. HbA1c was measured by high performance liquid chromatography(HPLC) with D-10 hemoglobin Testing Program (Bio-Rad). The serum insulin assay used magnetic beads-based enzymatic spectrofluorometric immunoassay with automatic enzyme immunoassay apparatus (AIA360, TOSOH). Serum glucose concentrations, total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDLC), low density lipoprotein cholesterol (LDLC), and serum creatinine(Scr) were measured with Hitachi Model 7600 Series Automatic Analyzer. Glomerular filtration rate(GFR) was estimated by using the reexpressed 4-variable Modification of Diet in Renal Disease (MDRD) Study equation (eGFR = 175 × (standardized Scr) –1.154 × age–0.203 × 0.742 [if female]) [22 (link)].
8
Blood Analysis of Mice
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Blood samples were collected before the mice were killed. The blood cell and biochemical analysis were performed using hematology analyzer (SYSMEX, XE-5000, Japan) and a biochemical analyzer (Hitachi Model 7600 Series Automatic Analyzer, Japan), respectively.
9
Comprehensive Metabolic and Cardiovascular Profiling
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Body mass index (BMI) was calculated (kg/m2). Systolic blood pressure (SBP) and diastolic blood pressure (DBP) taken three times using a sphygmomanometer and then averaged. Capillary glucose concentrations were measured with Lifescan Surestep blood glucose meter. Plasma glucose levels were measured using the glucose oxidase method. HbA1c was measured by high performance liquid chromatography (HPLC) with D-10 hemoglobin Testing Program (Bio-Rad). The serum insulin assay used magnetic beads-based enzymatic spectrofluorometric immunoassay with automatic enzyme immunoassay apparatus (AIA360, TOSOH). Serum glucose concentrations, total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDLC), and low density lipoprotein cholesterol (LDLC) were measured with Hitachi Model 7600 Series Automatic Analyzer.
10
Comprehensive Metabolic Profiling in Fasted Individuals
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Venous blood sample were collected in the morning following an overnight fast. Sociodemographic characteristics, physical examination information and laboratory measurements such as gender, age, durations, body mass index (BMI), serum uric acid (SUA), blood lipid, blood pressure, liver and kidney function parameters and blood glucose were collected by trained doctors. HbA1c was determined by high performance liquid chromatography (HLC-723G8 Automatic glycosylated hemoglobin Analyzer, TOSOH, Japan). Biochemical indexes were measured by automatic biochemical analyzer (Model 7600 Series Automatic Analyzer, Hitachi, Japan). Insulin and C-peptide concentrations were measured by electricity chemiluminescent immunoassay (IMMULITE 2000 XPi, Siemens, Germany) and glucagon was measured by radioimmunoassay (RIA, Millipore, Billerica, MA, USA). Serum OCN levels were evaluated by the electrochemiluminescence immunoassay method on a Roche COBAS E 801 (Roche Diagnostics Corporation, Mannheim, Germany).
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