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Fd2000s 1g

Manufactured by Merck Group
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The FD2000S-1G is a freeze dryer manufactured by Merck Group. It is a laboratory-grade equipment designed for the lyophilization of samples. The device utilizes a vacuum system and controlled temperature to remove water from frozen samples through the process of sublimation.

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Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Tetrabutylammonium hydroxide solution, by Merck Group (1 mentions) Anhydrous dimethyl sulfoxide, by Merck Group (1 mentions) 4 dimethylaminopyridine, by Merck Group (1 mentions) Di tert butyl dicarbonate, by Merck Group (1 mentions) Fluorescein isothiocyanate dextran, by Merck Group (1 mentions) 2 hydroxy 4 2 hydroxyethoxy 2 methylpropiophenone i2959, by Merck Group (1 mentions) Alexa fluor 647 phalloidin, by Thermo Fisher Scientific (1 mentions) 3 trimethoxysilyl propyl methacrylate tmspma, by Merck Group (1 mentions) 5 norbornene 2 carboxylic acid, by Merck Group (1 mentions) Gelma, by Avantor (1 mentions) Seakem agarose, by Avantor (1 mentions) Tetrabutylammonium hydroxide tba oh, by Merck Group (1 mentions) Sodium hyaluronate, by Lifecore Biomedical (1 mentions) Dl dithiothreitol, by Merck Group (1 mentions) Polyethylene oxide, by Merck Group (1 mentions) Lsm 880 microscope, by Zeiss (1 mentions)

5 protocols using fd2000s 1g

1

Biomimetic Hydrogel for Cell Culture

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All chemicals were used as received unless stated otherwise. Sodium hyaluronate (64 kDa) was purchased from Lifecore Biomedical (Chaska, MN). Dowex™ 500WX8–200 ion-exchange resin (AC335341000) was purchased from Fisher Scientific. GelMA (76292–720) and Seakem® Agarose (12002–102) were purchased from VWR. Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) was purchased from Colorado Photopolymer Solutions (Boulder, CO). Alexa Fluor™ 647 Phalloidin (A22287), LIVE/DEAD™ Viability/Cytotoxicity kit (L3224), and Hoechst 33342 (H3570) were purchased from Thermo Fisher Scientific. Tetrabutylammonium hydroxide (TBA-OH, 86880–100ML), anhydrous dimethyl sulfoxide (276855), 5-norbornene-2-carboxylic acid (446440), 4-(dimethylamino)pyridine (107700), di-tert-butyl dicarbonate (361941), 4-(dimethylamino)pyridine (107700), tetrabutylammonium hydroxide solution (86880), DL-dithiothreitol (D0632), poly(ethylene oxide) (189456), 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (I2959) (410896), fluorescein isothiocyanate-dextran (average molecular weight 2 MDa, FD2000S-1G) and 3-(trimethoxysilyl)propyl methacrylate (TMSPMA) (440159–500ML) were purchased from Sigma-Aldrich (St. Louis, MO).
Prendergast M.E., Davidson M.D, & Burdick J.A. (2021). A Biofabrication Method to Align Cells within Bioprinted Photocrosslinkable and Cell-degradable Hydrogel Constructs via Embedded Fibers. Biofabrication, 13(4), 10.1088/1758-5090/ac25cc.
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2

Cerebral Fluorescent Angiography in Mice

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Cerebral fluorescein isothiocyanate (FITC)-dextran fluorescent angiography was performed as described in detail previously [14 (link)] using FITC-dextran (FD2000S-1G; 2000 kDa; 0.1 mL 50 mg/mL in sterile saline; Sigma, St. Louis, MO, USA). Mice were euthanized without perfusion 5 min after injecting FITC-dextran via tail vein and whole brains removed and post-fixed in 4% PFA in PBS at 4 °C overnight. Sequential coronal sections (100 μm thickness) were processed using vibratome for fluorescent imaging.
Chen X., Chen L., Lin G., Wang Z., Kodali M.C., Li M., Chen H., Lebovitz S.G., Ortyl T.C., Li L., Ismael S., Singh P., Malik K.U., Ishrat T., Zhou F.M., Zheng W, & Liao F.F. (2022). White matter damage as a consequence of vascular dysfunction in a spontaneous mouse model of chronic mild chronic hypoperfusion with eNOS deficiency. Molecular Psychiatry, 27(11), 4754-4769.
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3

Visualizing Vascular Permeability in Mouse Eyes

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Fluorescein Isothiocyanate dextran (FITC-dextran: FD2000S-1G, Sigma) was diluted in distilled water to a concentration of 50 mg/ml. Mice were anesthetized, and 0.2 ml FITC-dextran was injected into the left ventricle of the mouse. Eight minutes later, the mice were sacrificed. Eyeballs were enucleated and fixed in 4% PFA for 1 h at room temperature. After washing in PBS, the eyeballs were incubated with 2% hydrogen peroxide (H2O2) for 15 min at room temperature and then 10% H2O2 at 55 ℃ for 2.5 h to bleach the pigment [20 (link)]. The eyeballs were washed in PBS, and the anterior segment and lens were removed. The RPE-choroid-sclera complex and the neural retina were prepared and mounted on glass slides. Fluorescent images were captured by a Zeiss LSM 880 microscope.
Yan M., Li J., Yan L., Li X, & Chen J.G. (2022). Transcription factor Foxp1 is essential for the induction of choroidal neovascularization. Eye and Vision, 9, 10.
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4

Cerebral Fluorescent Angiography in Mice

Cited 1 time
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Cerebral fluorescein isothiocyanate (FITC)-dextran fluorescent angiography was performed as described in detail previously [14 (link)] using FITC-dextran (FD2000S-1G; 2000 kDa; 0.1 mL 50 mg/mL in sterile saline; Sigma, St. Louis, MO, USA). Mice were euthanized without perfusion 5 min after injecting FITC-dextran via tail vein and whole brains removed and post-fixed in 4% PFA in PBS at 4 °C overnight. Sequential coronal sections (100 μm thickness) were processed using vibratome for fluorescent imaging.
Chen X., Chen L., Lin G., Wang Z., Kodali M.C., Li M., Chen H., Lebovitz S.G., Ortyl T.C., Li L., Ismael S., Singh P., Malik K.U., Ishrat T., Zhou F.M., Zheng W, & Liao F.F. (2022). White matter damage as a consequence of vascular dysfunction in a spontaneous mouse model of chronic mild chronic hypoperfusion with eNOS deficiency. Molecular Psychiatry, 27(11), 4754-4769.
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5

Cerebral FITC-Dextran Fluorescent Angiography

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Cerebral uorescein isothiocyanate (FITC)-dextran uorescent angiography was performed as described in detail previously [16] using FITC-dextran (FD2000S-1G; 2000 kDa; 0.1 mL 50 mg/mL in sterile saline; Sigma, St. Louis, MO, USA). Mice were euthanized without perfusion 5 min after injecting FITC-dextran via tail vein and whole brains removed and post-xed in 4 % PFA in PBS at 4°C overnight. Sequential coronal sections (100 mm thickness) were processed using vibratome for uorescent imaging.
Chen X., Chen L., Lin G., Kodali M.C., Li M., Lebovitz S.G., Ortyl T.C., Li L., Ismael S., Singh P., Malik K.U., Ishrat T.G., Zheng W., & Liao F. (2021). White Matter Damage as a Consequence of Vascular Dysfunction in a Spontaneous Mouse Model of Chronic Hypoperfusion with eNOS Deficiency.
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