Lysophosphatidic acid

Manufactured by Bio-Techne

Lysophosphatidic acid is a naturally occurring phospholipid that serves as a core component in the Bio-Techne product line. It functions as a precursor molecule in the synthesis of various lipid-based compounds.

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Lab products found in correlation

Bicinchoninic acid protein assay kit, by Merck Group (1 mentions) Ah6809, by Merck Group (1 mentions) Prostaglandin e1, by Santa Cruz Biotechnology (1 mentions) Prostaglandin e2, by Santa Cruz Biotechnology (1 mentions) L 798106, by Merck Group (1 mentions) 4 5 3h leucine, by PerkinElmer (1 mentions) Nsc23766, by Bio-Techne (1 mentions) Luciferase assay kit, by Promega (1 mentions) Cell culture reagents, by Thermo Fisher Scientific (1 mentions) Isoproterenol, by Merck Group (1 mentions) Sphingosine 1 phosphate (s1p), by Bio-Techne (1 mentions) Ipa 3, by Bio-Techne (1 mentions) Sq22536, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

1-Oleoyl lysophosphatidic acid (LPA, (3 citations) 1-Oleoyl lysophosphatidic acid sodium salt (2 citations)

2 protocols using lysophosphatidic acid

Quantifying RhoA Activation in mESCs

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mESCs were serum-starved for 24 hours, treated by lysophosphatidic acid (1 µg/ml; Tocris) for 3 min, and lysed on ice. Guanosine triphosphate (GTP)–RhoA was pulled down by immobilized rhotekin RhoA binding domain (Cytoskeleton) for 1 hour at 4°C. RhoA was detected by immunoblotting with anti-RhoA (Cytoskeleton).

Yang J., Wu C., Stefanescu I., Jakobsson L., Chervoneva I, & Horowitz A. (2016). RhoA inhibits neural differentiation in murine stem cells through multiple mechanisms. Science signaling, 9(438), ra76.

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Endothelin-1 Signaling Pathway Analysis

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Phenylephrine (PE), endothelin‐1 (ET1), dibutyryl‐cAMP, AH6809, L‐798106, isoproterenol, and bicinchoninic acid protein assay kit were purchased from Sigma‐Aldrich. Prostaglandin E1, prostaglandin E2, SQ 22536, and treprostinil were purchased from Santa Cruz, autocamtide‐2‐related inhibitory peptide II, L‐161,982, bisindolylmaleimide, and pertussis toxin from Calbiochem. Sphingosine‐1‐phosphate, lysophosphatidic acid, IPA‐3, and NSC23766 were from Tocris. Fluprostenol was from Cayman, and WIN55,212‐2 was from Enzo. Angiotensin II was bought from American Peptide, and the Luciferase Assay Kit was purchased from Promega. BPKDi was custom‐synthesized (Haworth et al, 2012). [4,5‐³H]‐leucine was from Perkin‐Elmer. Cell culture reagents were from Invitrogen.

Tóth A.D., Schell R., Lévay M., Vettel C., Theis P., Haslinger C., Alban F., Werhahn S., Frischbier L., Krebs‐Haupenthal J., Thomas D., Gröne H., Avkiran M., Katus H.A., Wieland T, & Backs J. (2018). Inflammation leads through PGE/EP 3 signaling to HDAC5/MEF2‐dependent transcription in cardiac myocytes. EMBO Molecular Medicine, 10(7), e8536.

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